Life Stage
Contents
Life_Stage Data Model
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?Life_stage Public_name UNIQUE Text //added by Daniela 120509 Contains Cell ?Cell XREF Life_stage Cell_group ?Cell_group XREF Life_stage Anatomy_term ?Anatomy_term XREF Life_stage Sub_stage ?Life_stage XREF Contained_in Contained_in ?Life_stage XREF Sub_stage // changed for Wen 010516 Preceded_by ?Life_stage XREF Followed_by Followed_by ?Life_stage XREF Preceded_by Expr_pattern ?Expr_pattern XREF Life_stage Remark ?Text #Evidence Definition ?Text // added for Wen [020607 krb] Other_name ?Text // added for Wen [020607 krb] Reference ?Paper XREF Life_stage Curated_by UNIQUE Text // added for Sylvia [010928 krb]
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obo file
https://raw.github.com/draciti/Life-stage-obo/master/worm_development.obo
Life_Stage
Originally Life_stage objects were stored as names. In May 2012 Caltech decided to switch Life_stage objects from names to IDs. In order to do that we need a model change to store the original name (see model).
All data types that were using Life_stage had to be modified and dumped into Ids instead of names
- Phenotype -Karen
- Process -Karen
- RNAi -Chris
- gene regulation -Xiaodong
- gene expression -Daniela
- Anatomy_term -Raymond
- Expression Cluster -Wen
- Condition -Wen & Gary W
- Strain -Mary Ann
Life_Stage tags
tags used in Life_stage extracted from the Life_Stage. ace dump that I got from Wen on May 9th 2012
- Definition
- Anatomy_term
- Reference
- Other_name
- Expr_pattern
- Contained_in -> Change data into IDs
- Cell_group
- Sub_stage -> Change data into IDs
- Cell
- Followed_by -> Change data into IDs
- Remark -> goid: WBls could be deleted because will be redundant with IDs
- Preceded_by -> Change data into IDs
Action items
.ace file to modify located here: /home/acedb/draciti/Life_stage and is named Life_stageWS230.ace
Modify the .ace dump as follows
1) Object should be replaced with ID instead of name using the mapping table
2) A new tag called Public_name should be added and should contain the name that was previously the object name
3) the following tags should be replaced with ID instead of name
- Contained_in
- Followed_by
- Precede_by
- Sub_stage
4) The lines containing Remark "goid: WBls:#######" should be deleted
Mapping table is here
http://tazendra.caltech.edu/~azurebrd/var/work/lifestage
script at : /home/acedb/draciti/Life_stage/parse.pl output goes to script. Errors from lack of mappings are in the file. current output in file "out"
the script that updates the OA runs at 8pm every day and is at
/home/postgres/work/pgpopulation/obo_oa_ontologies/update_obo_oa_ontologies.pl
gets the data from here https://raw.github.com/draciti/Life-stage-obo/master/worm_development.obo
The script runs when the date in the obo file changes.
Deleting terms
In WS230 the following terms were used in Citace Minus but were not present in the obo file:
L1 Larva Male L2 Larva male L3 Larva male
the last term generated was L4 Larva male:
[Term] id: WBls:0000073 name: L4 larva male def: "The fourth stage larva male. At 25 Centigrade\, it ranges 40-49.5 hours after fertilization\, 26-35.5 hours after hatch." [wb:wjc] is_a: WBls:0000038 ! L4 larva
I could have added the following but Raymond suggested to leave the sex distinction out of the ontology. We are therefore NOT adding these new terms. Checked in the Life stage WS230 dump -> no data associated with these terms, we could delete them from the obo file and modify citace minus accordingly. Waiting for Wen's feedback -> OK. I deleted the 3 L1 Larva Male, L2 Larva male, L3 Larva male, L4 larva hermaphrodite, mixed, mixed stage terms from Citace Minus
Deposited on spica (Data for CitaceMinus) the file Life_stageD with the corrections and the Name_to_ID change. (05152012DR) Create terms for one cell embryo and oocyte?
Adding terms
Added 11-15 days post-L4 adult hermaphrodite using obo edit as per Wen Chen request (WBPaper38462).
id: WBls:0000074 name: 11-15 days post-L4 adult hermaphrodite def: "At 20 Centigrade\\: 11-15 days after L4-adult molt. 14-18 days after first cleavage." [wb:dr] is_a: WBls:0000057 ! adult hermaphrodite created_by: danielaraciti creation_date: 2012-05-22T14:35:04Z
set up crosstalk between obo file and citace
Added preceded_by relationships in the obo file using the dump "LS_Preceded_by.ace" provided by Wen -DR. Added additional preceded_by relationships that were not in the file-DR. The file is located on Lario here /Users/danielaraciti/Desktop/Life_stage/Relationships.
The conversion from obo to .ace should be as follows
OBO id: WBls:0000007 name: 2-cell embryo def: "0-20min after first cleavage at 20 Centigrade. Contains 2 cells." [wb:wjc] is_a: WBls:0000005 ! blastula embryo relationship: preceded_by WBls:0000006 ! 1-cell embryo .ace Life_stage : "WBls:0000007" Public_name "2-cell embryo" Definition "0-20min after first cleavage at 20 Centigrade. Contains 2 cells." Contained_in "WBls:0000005" Preceded_by "WBls:0000006"
- script at :
/home/acedb/draciti/Life_stage/lifestageAceFromObo.pl
- generates :
/home/acedb/draciti/Life_stage/lifestage.ace
Here's the relevant matching : if ($obj =~ m/id: (WBls:\d+)/) { $id = $1; $ace .= qq(Life_stage : "$id"\n); if ($obj =~ m/name: (.*)/) { $ace .= qq(Public_name\t"$1"\n); } if ($obj =~ m/def: "(.*)"/) { $ace .= qq(Definition\t"$1"\n); } if ($obj =~ m/is_a: (WBls:\d+)/) { $ace .= qq(Contained_in\t"$1"\n); } if ($obj =~ m/relationship: preceded_by (WBls:\d+)/) { $ace .= qq(Preceded_by\t"$1"\n); } $ace .= qq(\n); meaning : - the id is the WBls - the name is everything - the def is what's in quotes - the is_a is the WBls - the relationship is the WBls and it must say "relationship: precededby WBls:\d+"
It always downloads the .obo file from the URL https://raw.github.com/draciti/Life-stage-obo/master/worm_development.obo, and always regenerates the .ace file.
Daniela runs the script at each upload and deposits the .ace file to citace
other species
please list below the life stages that have been used in the literature for other species. This list will be periodically revised in order to expand the life stage ontology
- C. briggsae
- ...
- C. japonica
- ...
- C. remanei
- ...
- C. sp11
- ...
- P. pacificus
- ...
- A. suum
- ...