Difference between revisions of "WormMine"

From WormBaseWiki
Jump to navigationJump to search
 
(109 intermediate revisions by 11 users not shown)
Line 1: Line 1:
 +
 
= Current status =
 
= Current status =
−
 
−
[http://206.108.125.166:8080/wormmine Link to mine]
 
  
 
{| class="wikitable" border="1"
 
{| class="wikitable" border="1"
 
|-
 
|-
−
|'''ONLINE'''
+
|'''
 +
ONLINE'''
 
|}
 
|}
  
 +
= Links =
 +
[http://intermine.wormbase.org/tools/wormmine/begin.do WormMine]
  
−
= Follow progress here: =
+
[https://github.com/WormBase/intermine WormMine Github]
−
[https://github.com/WormBase/intermine/commits/jw GitHub commit history]
 
  
−
= Account management =
+
[[UserGuide:WormMine|User Guide]]
 +
 
 +
= Logging in as admin =
 +
These specific URLs must be used since the admin account is a WormMine, not Google, account.
 +
 
 +
Login URL: http://www.wormbase.org/tools/wormmine/login.do
 +
 
 +
Logout URL: http://www.wormbase.org/tools/wormmine/logout.do
 +
 
 +
Find the admin account username and password by executing this search in your wormbase.org account:
 +
 
 +
<code>subject:[ WormMine ] Important update on status and curator action</code>
 +
 
 +
This account is needed to publish templates to the front page.
 +
 
 +
=New data wishlist=
 +
{| border="1"
 +
! Name !! Where is the data?
 +
|-
 +
| Orthology || ACeDB gene class (Ortholog and Ortholog_other tags) and there is also a OICR created flatfile)
 +
|-
 +
| Interaction || AceDB interaction class
 +
|-
 +
| Transgene ||Transgene AceDB class
 +
|-
 +
| variation coordinates || gff3, type SNP or SNP
 +
|-
 +
|Disease data || In AceDB class ?Gene, Import all data under the Disease_info tag
 +
|-
 +
|Motif Titles || In the "Title" tag of ?Motif
 +
|-
 +
|}
  
 
=Data contained in WormMine=
 
=Data contained in WormMine=
 
This lists all data sources contained in WormMine
 
This lists all data sources contained in WormMine
  
 +
Refer to species list:
 
{| border="1"
 
{| border="1"
−
! Source !! Description !! Source !! Species !! Data mapping
+
! taxonId !! species
 +
|-
 +
| 6253 || Ascaris suum
 +
|-
 +
| 6279 || Brugia malayi
 +
|-
 +
| 6326 || Bursaphelenchus xylophilus
 +
|-
 +
| 96668 (860376 according to NCBI) || Caenorhabditis angaria
 +
|-
 +
| 135651 || Caenorhabditis brenneri
 +
|-
 +
| 6238 || Caenorhabditis briggsae
 +
|-
 +
| 6239 || Caenorhabditis elegans
 +
|-
 +
| 281687 || Caenorhabditis japonica
 +
|-
 +
| 31234 || Caenorhabditis remanei
 +
|-
 +
| 6289 || Haemonchus contortus
 +
|-
 +
| 37862 || Heterorhabditis bacteriophora
 +
|-
 +
| 6305 || Meloidogyne hapla
 +
|-
 +
| 6306 || Meloidogyne incognita
 +
|-
 +
| 54126 || Pristionchus pacificus
 +
|-
 +
| 34506 || Strongyloides ratti
 +
|-
 +
| 6334 || Trichinella spiralis
 +
|}
 +
 
 +
 
 +
 
 +
{| border="1"
 +
! Source !! Description !! Source !! Species !! Filters !! Data mapping
  
 
|-
 
|-
 
|  GO  
 
|  GO  
 
|| Ontology terms and relationships comprising GO
 
|| Ontology terms and relationships comprising GO
−
|| GO project website.
+
|| GO project website (We should record the CVS revision number of the ontology file we use so we can perform data checks).
 
|| species neutral
 
|| species neutral
  
Line 31: Line 102:
 
|| GO project website.
 
|| GO project website.
 
|| Caenorhabditis elegans
 
|| Caenorhabditis elegans
 +
|| Removes all with first column = "UniProtKB"
  
 
|-
 
|-
Line 36: Line 108:
 
|| Fasta DNA sequences  
 
|| Fasta DNA sequences  
 
|| WormBase FTP
 
|| WormBase FTP
−
|| Caenorhabditis elegans
+
|| All in species list
  
 
|-
 
|-
Line 42: Line 114:
 
|| Fasta peptide sequences
 
|| Fasta peptide sequences
 
|| WormBase FTP
 
|| WormBase FTP
−
|| Caenorhabditis elegans
+
|| All in species list
  
 
|-
 
|-
Line 48: Line 120:
 
|| Gene chromosomal coordinates
 
|| Gene chromosomal coordinates
 
|| WormBase FTP GFF3
 
|| WormBase FTP GFF3
−
|| Caenorhabditis elegans
+
|| All in species list
  
 
|-
 
|-
Line 54: Line 126:
 
|| Transcript chromosomal coordinates
 
|| Transcript chromosomal coordinates
 
|| WormBase FTP GFF3
 
|| WormBase FTP GFF3
−
|| Caenorhabditis elegans
+
|| All in species list
  
 
|-
 
|-
Line 60: Line 132:
 
|| CDS chromosomal coordinates
 
|| CDS chromosomal coordinates
 
|| WormBase FTP GFF3
 
|| WormBase FTP GFF3
−
|| Caenorhabditis elegans
+
|| NONE (locations load improperly)
  
 
|-
 
|-
Line 67: Line 139:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/gene/mapping/wormbase-acedb-gene.properties LINK]
+
|| <code>query find Gene Live</code>
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Gene_mapping.properties LINK]
  
 
|-
 
|-
Line 74: Line 147:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/transcript/mapping/transcript_mapping.properties LINK]
+
|| <code>query find Transcript (Gene)</code>
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Transcript_mapping.properties LINK]
  
 
|-
 
|-
Line 81: Line 155:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/cds/mapping/cds_mapping.properties LINK]
+
|| <code>query find CDS Method="curated"</code>
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/CDS_mapping.properties LINK]
  
 
|-
 
|-
Line 88: Line 163:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/variation/mapping/variation_mapping.properties LINK]
+
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Variation_mapping.properties LINK]
  
 
|-
 
|-
Line 95: Line 171:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/protein/mapping/protein_mapping.properties LINK]
+
|| Limited to [https://github.com/WormBase/intermine/blob/jw/testlab/perl/preprocess/wb-acedb/protein/whitelist/species_whitelist.txt these] species,
 +
<code>query find Protein Corresponding_CDS</code>
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Protein_mapping.properties LINK]
  
 
|-
 
|-
Line 102: Line 180:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/phenotype/mapping/phenotype_mapping.properties LINK]
+
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Phenotype_mapping.properties LINK]
  
 
|-
 
|-
Line 109: Line 188:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/expr_pattern/mapping/expr_pattern_mapping.properties LINK]
+
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Expr_pattern_mapping.properties LINK]
  
 
|-
 
|-
Line 116: Line 196:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/anatomy_term/mapping/anatomy_term_mapping.properties LINK]
+
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Anatomy_term_mapping.properties LINK]
  
 
|-
 
|-
Line 123: Line 204:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/expr_cluster/mapping/expr_cluster_mapping.properties LINK]
+
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Expression_cluster_mapping.properties LINK]
  
 
|-
 
|-
Line 130: Line 212:
 
|| AceDB XML dump
 
|| AceDB XML dump
 
|| All in AceDB
 
|| All in AceDB
−
|| [https://github.com/WormBase/intermine/blob/jw/datadir/wormbase-acedb/life_stage/mapping/life_stage_mapping.properties LINK]
+
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Life_stage_mapping.properties LINK]
 +
 
 +
|-
 +
|  Species data
 +
|| Name and Taxon ID
 +
|| AceDB XML dump
 +
|| All in AceDB
 +
|| [https://github.com/WormBase/website-intermine/blob/master/acedb-dev/acedb/species_WS238.ace Species list]
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/Species_mapping.properties LINK]
 +
 
 +
|-
 +
|  RNAi metadata
 +
|| Select fields extracted from Ace
 +
|| AceDB XML dump
 +
|| All in AceDB
 +
||
 +
|| [https://github.com/WormBase/intermine/blob/unmerged/build_config/wormbase-acedb/RNAi_mapping.properties LINK]
  
 
|}
 
|}
Line 141: Line 240:
 
The data contained in WormMine follows a central model schema.  This model should be understood sufficiently to be able to query the data and create templates.
 
The data contained in WormMine follows a central model schema.  This model should be understood sufficiently to be able to query the data and create templates.
  
−
[https://github.com/WormBase/intermine/blob/jw/wormmine/dbmodel/build/model/genomic_model.xml WormMine model file]
+
[http://www.wormbase.org/tools/wormmine/service/model WormMine model file]
  
 
This schema file contains all of the data types contained in WormMine, relationships between them, and each one's data fields.   
 
This schema file contains all of the data types contained in WormMine, relationships between them, and each one's data fields.   
Line 193: Line 292:
  
 
=Using QueryBuilder=
 
=Using QueryBuilder=
 +
 
[[File:Querybuilder link.png]]
 
[[File:Querybuilder link.png]]
  
Line 204: Line 304:
 
==== Desired results? ====
 
==== Desired results? ====
 
We want to display the symbols of each involved object, plus the gene ID.  This calls for:
 
We want to display the symbols of each involved object, plus the gene ID.  This calls for:
−
# Gene.primaryIdentifier
+
# Gene.WormBase Gene ID
−
# Gene.symbol
+
# Gene.Gene Name
−
# Gene (some relationship(s))-> Transcript.symbol
+
# Gene (some relationship(s))-> Transcript.Sequence Name
−
# Gene (some relationship(s))-> CDS.symbol
+
# Gene (some relationship(s))-> CDS.Sequence Name
−
# Gene (some relationship(s))-> Protein.symbol
+
# Gene (some relationship(s))-> Protein.Name
  
 
==== Building the query ====
 
==== Building the query ====
−
[[File:Qb circled.png|frame|click these areas]]
+
[[File:QB_circled_new.png|frame|click these areas]]
−
The DB Identifier represents the primaryIdentifier, this is the only field name replaced in this way. Click show next to any of these field names to add that attribute to the result table as a column.
+
Click *SHOW* next to any of these field names to add that attribute to the resulting table as a column.
−
* Show the primaryIdentifier (DB Identifier), and symbol
+
* Show the "WormBase Gene ID" and "Gene Name"
 
* Gene contains a collection of transcripts, this relationship represents transcription.  Expand the relationship by clicking on the <code>[+]</code>
 
* Gene contains a collection of transcripts, this relationship represents transcription.  Expand the relationship by clicking on the <code>[+]</code>
 
<br clear=all>
 
<br clear=all>
−
* Follow these steps for each of the data types in the chain as illustrated.
 
−
[[File:Tscript expanded2.png|thumb]]
 
−
Running the query at this point will give you results for ''all'' genes.  If you have a smaller set in mind, like restricting the genes to AAP-1 only, a constraint must be set.
 
−
* Click "constrain" next to the Gene.symbol field (circle #2), type "aap-1" in the text box, make sure the operator is "=", then "add to query".
 
  
−
Your query overview should resemble this:
+
 
−
[[File:Gene prot aap1.png|frame|left]]
+
* Follow these steps for each of the data types in the chain as illustrated:
−
[[File:Cds expanded.png|thumb]]
+
 
−
[[File:Protein expanded.png|thumb]]
+
[[File:Tscript_expanded2_new2.png|frame|left]]
 +
[[File:CDS_expanded_new2.png|frame]]
 +
[[File:Protein_expanded_new2.png|frame|left]]
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
* Running the query at this point will give you results for ''all'' genes.  If you have a smaller set in mind, like restricting the genes to egl-19 only, a constraint must be set.
 +
* Click "constrain" next to the "Gene.Gene Name" field (circle #2), type "egl-19" in the text box, make sure the operator is "=", then "add to query".
 +
 
 +
 
 +
[[File:Constraint_Dialog_Box_for_egl-19.png|frame]]
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
* Your query overview should resemble this:
 +
 
 +
 
 +
[[File:QB_egl-19_new.png|frame|left]]
 +
 
 
<br clear=all>
 
<br clear=all>
  
 
* Show results will execute the completed query.
 
* Show results will execute the completed query.
 
<br clear=all>
 
<br clear=all>
 +
 +
=== constraining to a list ===
 +
If you have access to any lists, the constraint dialog box will provide options with respect to them.  Fields can be constrained such that their values must or mustn't be a member of that list.
  
 
=Creating templates=
 
=Creating templates=
 +
Template queries are predefined (canned) queries. A template query can address a specific question and it can also be a good jump off point for refinements and for constructing queries that answer related questions. To create a template:
 +
 +
* Log in
 +
* Construct a query using Query Builder
 +
* Must include at least one constrain condition (e.g. restrict the format of the identifier)
 +
* "Start building a template query"
 +
* Fill in Name, Title and Description, and optionally comment
 +
* Make necessary adjustments, then "Save template"
 +
* Admin users can make a personal template '''public''' by
 +
  My Mine -> Templates -> Add Tags -> New tag, type in the text box '''"im:public"'''.
 +
  Add one tag at a time.
 +
  Tags help to group lists.
 +
  For the list to be viewable by the '''Public''', add a tag '''"im:public"'''.    For the list to be grouped under the "aspect" categories on the Home page, add the two tags: '''"im:frontpage"''' and '''"im:aspect:ACPECTNAME"'''
 +
  The capitalisation of the ACPECTNAME that you use in the above tag is important. The word ASPECTNAME in the above tag should be replaced by one of '''Genomics''', '''Proteins''', '''Expression''', '''Genetic Variations''', '''Phenotypes''', '''Gene Ontology''', '''Strains'''
 +
  Hit "Create". '''(This tag should be available for the next template you create)'''.
 +
  The Name field text should start with your initials to identify you as the author e.g. "XYZ Gene Homology"
 +
  The Title field text is what the users see and should include '-->' which is converted into am arrow icon on the final template list on the Home page.
 +
 
=Creating lists=
 
=Creating lists=
 +
 +
A list is a collection of identifiers of the same type of entities (e.g. genes, proteins, body parts). Lists can be compared and combined and be used as the starting points of queries. Sources of lists may be external (from a third party resource) or from WormMine. To generate a list and make it public:
 +
 +
1. Log in.
 +
2. Make a query (Query Builder).
 +
3. On the Result page, top right corner, select "Create / Add to List" -> "Create New List" -> "All of Columns ...", or
 +
    "Choose individual items from the table". "Choose individual items from the table" allows further refinement.
 +
4. Provide for the list: a Name, an informative Description, and Tags.
 +
    The Name should start with your initials to identify you as the author e.g. "XYZ Gene Homology"
 +
5. Descriptions and tags can also be edited after a list is saved.
 +
 +
=Internal Report Page=
 +
find intermine internal id via a query (must be logged in as a super user)
 +
 +
http://206.108.125.166:8080/wormmine/report.do?id=
 +
 +
=Other Sites=
 +
 +
Tutorial on making templates http://www.yeastgenome.org/help/video-tutorials/yeastmine

Latest revision as of 16:16, 15 October 2020

Current status

ONLINE

Links

WormMine

WormMine Github

User Guide

Logging in as admin

These specific URLs must be used since the admin account is a WormMine, not Google, account.

Login URL: http://www.wormbase.org/tools/wormmine/login.do

Logout URL: http://www.wormbase.org/tools/wormmine/logout.do

Find the admin account username and password by executing this search in your wormbase.org account:

subject:[ WormMine ] Important update on status and curator action

This account is needed to publish templates to the front page.

New data wishlist

Name Where is the data?
Orthology ACeDB gene class (Ortholog and Ortholog_other tags) and there is also a OICR created flatfile)
Interaction AceDB interaction class
Transgene Transgene AceDB class
variation coordinates gff3, type SNP or SNP
Disease data In AceDB class ?Gene, Import all data under the Disease_info tag
Motif Titles In the "Title" tag of ?Motif

Data contained in WormMine

This lists all data sources contained in WormMine

Refer to species list:

taxonId species
6253 Ascaris suum
6279 Brugia malayi
6326 Bursaphelenchus xylophilus
96668 (860376 according to NCBI) Caenorhabditis angaria
135651 Caenorhabditis brenneri
6238 Caenorhabditis briggsae
6239 Caenorhabditis elegans
281687 Caenorhabditis japonica
31234 Caenorhabditis remanei
6289 Haemonchus contortus
37862 Heterorhabditis bacteriophora
6305 Meloidogyne hapla
6306 Meloidogyne incognita
54126 Pristionchus pacificus
34506 Strongyloides ratti
6334 Trichinella spiralis


Source Description Source Species Filters Data mapping
GO Ontology terms and relationships comprising GO GO project website (We should record the CVS revision number of the ontology file we use so we can perform data checks). species neutral
GO Annotations Relationships between Genes and GO GO project website. Caenorhabditis elegans Removes all with first column = "UniProtKB"
Genomic sequences Fasta DNA sequences WormBase FTP All in species list
Protein sequences Fasta peptide sequences WormBase FTP All in species list
Gene locations Gene chromosomal coordinates WormBase FTP GFF3 All in species list
Transcript locations Transcript chromosomal coordinates WormBase FTP GFF3 All in species list
CDS locations CDS chromosomal coordinates WormBase FTP GFF3 NONE (locations load improperly)
Gene metadata Select fields extracted from Ace AceDB XML dump All in AceDB query find Gene Live LINK
Transcript metadata Select fields extracted from Ace AceDB XML dump All in AceDB query find Transcript (Gene) LINK
CDS metadata Select fields extracted from Ace AceDB XML dump All in AceDB query find CDS Method="curated" LINK
Variation metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK
Protein metadata Select fields extracted from Ace AceDB XML dump All in AceDB Limited to these species,

query find Protein Corresponding_CDS

LINK
Phenotype metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK
Expression Pattern metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK
Anatomy Term metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK
Expression Cluster metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK
Life Stage metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK
Species data Name and Taxon ID AceDB XML dump All in AceDB Species list LINK
RNAi metadata Select fields extracted from Ace AceDB XML dump All in AceDB LINK

What is this data mapping?

A loading program plugin has been created for InterMine which extracts data embedded in XML files directly into an InterMine instance. Mapping files are used to configure this program and detail the AceDB XML dumps to InterMine translation. XPath is used to query the XML, and can be reviewed here.

Understanding our model

The data contained in WormMine follows a central model schema. This model should be understood sufficiently to be able to query the data and create templates.

WormMine model file

This schema file contains all of the data types contained in WormMine, relationships between them, and each one's data fields.

How to read it

Looking at the protein class: 

   <class name="Protein" extends="BioEntity" is-interface="true">
       <attribute  name="molecularWeight" type="java.lang.Float"/>
       <attribute  name="md5checksum" type="java.lang.String"/>
       <attribute  name="length" type="java.lang.Integer"/>
       <attribute  name="geneName" type="java.lang.String"/>
       <attribute  name="primaryAccession" type="java.lang.String"/>
       <reference  name="sequence" referenced-type="Sequence"/>
       <collection name="CDSs" referenced-type="CDS" reverse-reference="protein"/>
       <collection name="genes" referenced-type="Gene" reverse-reference="proteins"/>
       <collection name="transcripts" referenced-type="Transcript" reverse-reference="protein"/>
   </class>

Line by line:

<class name="Protein" extends="BioEntity" is-interface="true">

extends="BioEntity": Protein is a child of BioEntity therefore it inherits BioEntity's data fields.

Protein's parent, BioEntity: 

   <class name="BioEntity" is-interface="true">
       <attribute name="secondaryIdentifier" type="java.lang.String"/>
       <attribute name="symbol" type="java.lang.String"/>
       <attribute name="primaryIdentifier" type="java.lang.String"/>
       <attribute name="lastUpdated" type="java.util.Date"/>
       <attribute name="name" type="java.lang.String"/>
       <reference name="organism" referenced-type="Organism"/>
       <collection name="synonyms" referenced-type="Synonym" reverse-reference="subject"/>
       <collection name="publications" referenced-type="Publication" reverse-reference="bioEntities"/>
       <collection name="ontologyAnnotations" referenced-type="OntologyAnnotation" reverse-reference="subject"/>
       <collection name="phenotypesObserved" referenced-type="Phenotype" reverse-reference="observedIn"/>
       <collection name="phenotypesNotObserved" referenced-type="Phenotype" reverse-reference="notObservedIn"/>
       <collection name="crossReferences" referenced-type="CrossReference" reverse-reference="subject"/>
       <collection name="dataSets" referenced-type="DataSet" reverse-reference="bioEntities"/>
       <collection name="locatedFeatures" referenced-type="Location" reverse-reference="locatedOn"/>
       <collection name="locations" referenced-type="Location" reverse-reference="feature"/>
   </class>

Protein contains copies of all these attributes, references, and collections for itself. If BioEntity inherits any fields itself, those are included as well.

<attribute name="primaryAccession" type="java.lang.String"/>: This creates an attribute of protein called primaryAccession (read primary accession) which is a string (word(s)). This line enables every protein object to hold a primaryAccession value in addition to any children which may inherit from it.

<reference name="sequence" referenced-type="Sequence"/>: This creates a reference named "sequence" to another data type, in this case Sequence. Only one sequence object can be referenced this way at a time. reverse-reference attributes may appear here, which matches the reciprocal relationship in the referenced type if one exists.

<collection name="CDSs" referenced-type="CDS" reverse-reference="protein"/>: CDSs collection. It can hold many references to CDSs, which in return are stored in the CDS "protein" (CDS.protein) field.

Using QueryBuilder

Querybuilder link.png

Use case: How do I find all proteins for a gene?

How are they intuitively connected? Genes are transcribed into transcripts which contain coding sequences which are translated into proteins. Keep this in mind while constructing a query.

  • Select "Gene" in the box under "Select a Data Type to Begin a Query"
  • Click "Select", or double click "Gene"

This list represents the Gene data available to display. Various fields can be chosen to be shown in the results table, links to other data types followed, and filters can be set to constrain the results.

Desired results?

We want to display the symbols of each involved object, plus the gene ID. This calls for:

  1. Gene.WormBase Gene ID
  2. Gene.Gene Name
  3. Gene (some relationship(s))-> Transcript.Sequence Name
  4. Gene (some relationship(s))-> CDS.Sequence Name
  5. Gene (some relationship(s))-> Protein.Name

Building the query

click these areas

Click *SHOW* next to any of these field names to add that attribute to the resulting table as a column.

  • Show the "WormBase Gene ID" and "Gene Name"
  • Gene contains a collection of transcripts, this relationship represents transcription. Expand the relationship by clicking on the [+]



  • Follow these steps for each of the data types in the chain as illustrated:
Tscript expanded2 new2.png
CDS expanded new2.png
Protein expanded new2.png



















  • Running the query at this point will give you results for all genes. If you have a smaller set in mind, like restricting the genes to egl-19 only, a constraint must be set.
  • Click "constrain" next to the "Gene.Gene Name" field (circle #2), type "egl-19" in the text box, make sure the operator is "=", then "add to query".


Constraint Dialog Box for egl-19.png












  • Your query overview should resemble this:


QB egl-19 new.png


  • Show results will execute the completed query.


constraining to a list

If you have access to any lists, the constraint dialog box will provide options with respect to them. Fields can be constrained such that their values must or mustn't be a member of that list.

Creating templates

Template queries are predefined (canned) queries. A template query can address a specific question and it can also be a good jump off point for refinements and for constructing queries that answer related questions. To create a template: 

* Log in
* Construct a query using Query Builder
* Must include at least one constrain condition (e.g. restrict the format of the identifier)
* "Start building a template query"
* Fill in Name, Title and Description, and optionally comment
* Make necessary adjustments, then "Save template"
* Admin users can make a personal template public by
  My Mine -> Templates -> Add Tags -> New tag, type in the text box "im:public".
  Add one tag at a time. 
  Tags help to group lists.
  For the list to be viewable by the Public, add a tag "im:public".    For the list to be grouped under the "aspect" categories on the Home page, add the two tags: "im:frontpage" and "im:aspect:ACPECTNAME"
  The capitalisation of the ACPECTNAME that you use in the above tag is important. The word ASPECTNAME in the above tag should be replaced by one of Genomics, Proteins, Expression, Genetic Variations, Phenotypes, Gene Ontology, Strains
  Hit "Create". (This tag should be available for the next template you create).
  The Name field text should start with your initials to identify you as the author e.g. "XYZ Gene Homology"
  The Title field text is what the users see and should include '-->' which is converted into am arrow icon on the final template list on the Home page.

Creating lists

A list is a collection of identifiers of the same type of entities (e.g. genes, proteins, body parts). Lists can be compared and combined and be used as the starting points of queries. Sources of lists may be external (from a third party resource) or from WormMine. To generate a list and make it public: 

1. Log in.
2. Make a query (Query Builder).
3. On the Result page, top right corner, select "Create / Add to List" -> "Create New List" -> "All of Columns ...", or
   "Choose individual items from the table". "Choose individual items from the table" allows further refinement.
4. Provide for the list: a Name, an informative Description, and Tags. 
   The Name should start with your initials to identify you as the author e.g. "XYZ Gene Homology"
5. Descriptions and tags can also be edited after a list is saved.

Internal Report Page

find intermine internal id via a query (must be logged in as a super user)

http://206.108.125.166:8080/wormmine/report.do?id=

Other Sites

Tutorial on making templates http://www.yeastgenome.org/help/video-tutorials/yeastmine

Retrieved from "https://wiki.wormbase.org/index.php?title=WormMine&oldid=35888"