From WormBaseWiki
Jump to navigationJump to search

INDI (Interesting, Not-yet-modelled Data Index) is a collection of data types that are of significant scientific value pending a proper data model to allow annotation.

Chemical Site of Action

  • WBPaper00041675 Ca++ affects AWC-ON/OFF cell fate determination. By clamping intracellular Ca++ levels in specific cells using a human calcium-buffer protein, it was demonstrated that Ca++ has autonomous as well as non-autonomous roles.

C. elegans used as heterologous expression system

  • WBPaper00041951 Characterization of heat shock protein 70 gene from Haemonchus contortus and its expression and promoter analysis in Caenorhabditis elegans.

Laser ablation in P. pacificus

  • WBPaper00027138 Laser ablated Z1 and Z4 and assayed SM migration.

Mitochondrial / Nuclear mosaics

  • WBPaper00044860 A transmitochondrial cybrid worm strain, chpIR (M, CB4856>N2), was bred as homoplasmic for the CB4856 mtDNA genome in the N2 nuclear background. The cybrid strain also displayed significantly increased CIV activity, demonstrating this difference results from the mtDNA-encoded p.A12S variant.

Cytoplasm / Nuclear mosaics

  • WBPaper00046106 CMK-1 Functions Antagonistically in the Cytoplasm and in the Nucleus to Control Thermal Avoidance

Activity measurements of neuron

  • WBPaper00045009 Here, we studied AVA activity with and without DA stimulation. As shown in Figure 6A, DA reduced AVA activity in naive animals, whereas we observed a marked genotype-independent DA-induced increase in Ca2+ transients after conditioning.

Phenotypes of introgressed regions- Loci involved in hybrid incompatibility

  • WBPaper00046462 The recent discovery of a C. briggsae sister species, C. nigoni, has

opened up the possibility of dissecting the genetic basis of HI in nematode species. However, the paucity of dominant and visible marker prevents the efficient mapping of HI loci between the two species. To elucidate the genetic basis of speciation in nematode species, we first generated 96 chromosomally integrated GFP markers in the C. briggsae genome and mapped them into the defined locations by PCR and Next-Generation Sequencing (NGS). Aided by the marker, we backcrossed the GFP-associated C. briggsae genomic fragments into C. nigoni for at least 15 generations and produced 111 independent introgressions. The introgression fragments cover most of the C. briggsae genome. We finally dissected the patterns of HI by scoring the embryonic lethality, larval arrest, sex ratio and male sterility for each introgression line, through which we identified pervasive HI loci and produced a genome-wide landscape of HI between the two nematode species, the first of its type for any non-Drosophila species.

I've curated the integrated transgenes in C. briggsae. I can assign phenotypes to the created introgressed lines through attaching them to Strain, but, 1) the strains do not exist in WB 2) is there a better way of annotating these loci?

(Extra)Cellular component function

  • WBPaper00044848 Ablation of the midbody remnant or perturbation of rotational cortical flow reveals that microtubule-midbody remnant contacts are crucial for P1 spindle rotation and dorsoventral axis formation.