WS172
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Jump to navigationJump to searchNew release of WormBase WS172, Wormpep172 and Wormrna172 Fri Mar 2 16:35:57 GMT 2007 WS172 was built by Mary Ann, with a little help from her friends ====================================================================== This directory includes: i) database.WS172.*.tar.gz - compressed data for new release ii) models.wrm.WS172 - the latest database schema (also in above database files) iii) CHROMOSOMES/subdir - contains 3 files (DNA, GFF & AGP per chromosome) iv) WS172-WS171.dbcomp - log file reporting difference from last release v) wormpep172.tar.gz - full Wormpep distribution corresponding to WS172 vi) wormrna172.tar.gz - latest WormRNA release containing non-coding RNA's in the genome vii) confirmed_genes.WS172.gz - DNA sequences of all genes confirmed by EST &/or cDNA viii) cDNA2orf.WS172.gz - Latest set of ORF connections to each cDNA (EST, OST, mRNA) ix) gene_interpolated_map_positions.WS172.gz - Interpolated map positions for each coding/RNA gene x) clone_interpolated_map_positions.WS172.gz - Interpolated map positions for each clone xi) best_blastp_hits.WS172.gz - for each C. elegans WormPep protein, lists Best blastp match to human, fly, yeast, C. briggsae, and SwissProt & TrEMBL proteins. xii) best_blastp_hits_brigprot.WS172.gz - for each C. briggsae protein, lists Best blastp match to human, fly, yeast, C. elegans, and SwissProt & TrEMBL proteins. xiii) geneIDs.WS172.gz - list of all current gene identifiers with CGC & molecular names (when known) xiv) PCR_product2gene.WS172.gz - Mappings between PCR products and overlapping Genes Release notes on the web: ------------------------- http://www.wormbase.org/wiki/index.php/Release_notes Genome sequence composition: ---------------------------- WS172 WS171 change ---------------------------------------------- a 32365889 32365889 +0 c 17779856 17779856 +0 g 17756016 17756016 +0 t 32365689 32365689 +0 n 0 0 +0 Total 100267450 100267450 +0 Chromosomal Changes: -------------------- There are no changes to the chromosome sequences in this release. Gene data set (Live C.elegans genes 23996) ------------------------------------------ Molecular_info 22302 (92.9%) Concise_description 4445 (18.5%) Reference 6923 (28.9%) CGC_approved Gene name 8972 (37.4%) RNAi_result 19843 (82.7%) Microarray_results 19137 (79.8%) SAGE_transcript 20053 (83.6%) Wormpep data set: ---------------------------- There are 20105 CDS in autoace, 23249 when counting 3144 alternate splice forms. The sequences contain 10,196,157 base pairs in total. Modified entries 27 Deleted entries 10 New entries 33 Reappeared entries 0 Net change +23 Status of entries: Confidence level of prediction (based on the amount of transcript evidence) ------------------------------------------------- Confirmed 7803 (33.5%) Every base of every exon has transcription evidence (mRNA, EST etc.) Partially_confirmed 10805 (46.5%) Some, but not all exon bases are covered by transcript evidence Predicted 4641 (20.0%) No transcriptional evidence at all Status of entries: Protein Accessions ------------------------------------- UniProtKB/Swiss-Prot accessions 3504 (15.1%) UniProtKB/TrEMBL accessions 19459 (83.7%) Status of entries: Protein_ID's in EMBL --------------------------------------- Protein_id 22963 (98.8%) Gene <-> CDS,Transcript,Pseudogene connections (cgc-approved) --------------------------------------------- Entries with CGC-approved Gene name 7329 GeneModel correction progress WS171 -> WS172 ----------------------------------------- Confirmed introns not in a CDS gene model; +---------+--------+ | Introns | Change | +---------+--------+ Cambridge | 185 | 170 | St Louis | 214 | 201 | +---------+--------+ Members of known repeat families that overlap predicted exons; +---------+--------+ | Repeats | Change | +---------+--------+ Cambridge | 6 | 0 | St Louis | 6 | 0 | +---------+--------+ Synchronisation with GenBank / EMBL: ------------------------------------ No synchronisation issues There are no gaps remaining in the genome sequence --------------- For more info mail help@wormbase.org -===================================================================================- New Data: --------- Genome sequence updates: ----------------------- New Fixes: ---------- There were several thousand EST's in the database that did not have orientation information. This has been added for this release and so the ESTs have been incorporated in to the Coding_transcript building procedures resulting in many more variants. We are continually working on cleaning the transcript data to make sure that short TSL and poly sequences are correctly identified to reduce the number of abherent transcripts produced. Known Problems: --------------- Other Changes: -------------- Proposed Changes / Forthcoming Data: ------------------------------------- The Sanger Institue and Genome Sequencing Center, Washington U. have exchanged ownership of a handful of clones. The genome sequencing project originally divided each chromosome roughly in half with one centre doing each. A small number of clones ended up being sequenced in the other location. This change is just to make the annotation and storage of the data easier and should be transparent to all users. The original Genbank / EMBL accessions will become seconary, with new ones assigned by the database now holding the record. Model Changes: ------------------------------------ -===================================================================================- Quick installation guide for UNIX/Linux systems ----------------------------------------------- 1. Create a new directory to contain your copy of WormBase, e.g. /users/yourname/wormbase 2. Unpack and untar all of the database.*.tar.gz files into this directory. You will need approximately 2-3 Gb of disk space. 3. Obtain and install a suitable acedb binary for your system (available from www.acedb.org). 4. Use the acedb 'xace' program to open your database, e.g. type 'xace /users/yourname/wormbase' at the command prompt. 5. See the acedb website for more information about acedb and using xace. ____________ END _____________