Difference between revisions of "WS154"
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There are no gaps remaining in the genome sequence | There are no gaps remaining in the genome sequence | ||
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− | For more info mail | + | For more info mail help@wormbase.org |
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Latest revision as of 11:08, 21 December 2011
Contents
Release Letter
New release of WormBase WS154, Wormpep154 and Wormrna154 Fri Feb 10 12:46:08 GMT 2006 WS154 was built by Paul Davis ====================================================================== This directory includes: i) database.WS154.*.tar.gz - compressed data for new release ii) models.wrm.WS154 - the latest database schema (also in above database files) iii) CHROMOSOMES/subdir - contains 3 files (DNA, GFF & AGP per chromosome) iv) WS154-WS153.dbcomp - log file reporting difference from last release v) wormpep154.tar.gz - full Wormpep distribution corresponding to WS154 vi) wormrna154.tar.gz - latest WormRNA release containing non-coding RNA's in the genome vii) confirmed_genes.WS154.gz - DNA sequences of all genes confirmed by EST &/or cDNA viii) cDNA2orf.WS154.gz - Latest set of ORF connections to each cDNA (EST, OST, mRNA) ix) gene_interpolated_map_positions.WS154.gz - Interpolated map positions for each coding/RNA gene x) clone_interpolated_map_positions.WS154.gz - Interpolated map positions for each clone xi) best_blastp_hits.WS154.gz - for each C. elegans WormPep protein, lists Best blastp match to human, fly, yeast, C. briggsae, and SwissProt & TrEMBL proteins. xii) best_blastp_hits_brigprot.WS154.gz - for each C. briggsae protein, lists Best blastp match to human, fly, yeast, C. elegans, and SwissProt & TrEMBL proteins. xiii) geneIDs.WS154.gz - list of all current gene identifiers with CGC & molecular names (when known) xiv) PCR_product2gene.WS154.gz - Mappings between PCR products and overlapping Genes Release notes on the web: ------------------------- http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE Primary databases used in build WS154 ------------------------------------ brigdb : 2004-03-12 camace : 2006-01-23 - updated citace : 2006-01-20 - updated cshace : genace : 2006-01-23 - updated stlace : 2006-01-20 - updated Genome sequence composition: ---------------------------- WS154 WS153 change ---------------------------------------------- a 32365775 32365775 +0 c 17779813 17779813 +0 g 17755968 17755968 +0 t 32365578 32365578 +0 n 0 0 +0 - 0 0 +0 Total 100267134 100267134 +0 Gene data set (Live C.elegans genes 23707) ------------------------------------------ Molecular_info 21956 (92.6%) Concise_description 4131 (17.4%) Reference 4885 (20.6%) CGC_approved Gene name 8710 (36.7%) RNAi_result 19780 (83.4%) Microarray_results 19108 (80.6%) SAGE_transcript 18197 (76.8%) Wormpep data set: ---------------------------- There are 20060 CDS in autoace, 22943 when counting 2883 alternate splice forms. The 22943 sequences contain 10,080,578 base pairs in total. Modified entries 79 Deleted entries 40 New entries 82 Reappeared entries 0 Net change +42 Status of entries: Confidence level of prediction (based on the amount of transcript evidence) ------------------------------------------------- Confirmed 6584 (28.7%) Every base of every exon has transcription evidence (mRNA, EST etc.) Partially_confirmed 11410 (49.7%) Some, but not all exon bases are covered by transcript evidence Predicted 4949 (21.6%) No transcriptional evidence at all Status of entries: Protein Accessions ------------------------------------- UniProtKB/Swiss-Prot accessions 3139 (13.7%) UniProtKB/TrEMBL accessions 19577 (85.3%) Status of entries: Protein_ID's in EMBL --------------------------------------- Protein_id 22716 (99.0%) Gene <-> CDS,Transcript,Pseudogene connections (cgc-approved) --------------------------------------------- Entries with CGC-approved Gene name 7021 GeneModel correction progress WS153 -> WS154 ----------------------------------------- Confirmed introns not in a CDS gene model; +---------+--------+ | Introns | Change | +---------+--------+ Cambridge | 126 | -10 | St Louis | 7 | 1 | +---------+--------+ Members of known repeat families that overlap predicted exons; +---------+--------+ | Repeats | Change | +---------+--------+ Cambridge | 585 | -5 | St Louis | 761 | -3 | +---------+--------+ Synchronisation with GenBank / EMBL: ------------------------------------ No synchronisation issues There are no gaps remaining in the genome sequence --------------- For more info mail help@wormbase.org -===================================================================================- New Data: --------- 1) Vancouver (VRM) Fosmid library is now mapped to the current elegans assembly and will be available to view in the genome browser. 2) 1215 new RNAi objects. 3) >500 new variations. New Fixes: ---------- 1) Mitochondrial gene predictions will now display correctly within wormbase and the genome browser. gff is available for all CDSs/Coding_Transcripts/WBgene_spans. 2) The clone C24G6 had incorrect wublastx data in the previous release of WormBase (WS153) this has now been resolved. Known Problems: -------------- Other Changes: -------------- Proposed Changes / Forthcoming Data: ------------------------------------ 1) Gene -> WRM Fosmid library connections will be generated to speed up fosmid identification. Model Changes: ------------------------------------ 1) Removed Interpolated_map_position from ?CDS, ?Transcript and ?Pseudogene. 2) Changed ?Variation Interpolated_map_position to be same structure and ?Gene ie Interpolated_map_position UNIQUE ?Map UNIQUE Float -===================================================================================- Quick installation guide for UNIX/Linux systems ----------------------------------------------- 1. Create a new directory to contain your copy of WormBase, e.g. /users/yourname/wormbase 2. Unpack and untar all of the database.*.tar.gz files into this directory. You will need approximately 2-3 Gb of disk space. 3. Obtain and install a suitable acedb binary for your system (available from www.acedb.org). 4. Use the acedb 'xace' program to open your database, e.g. type 'xace /users/yourname/wormbase' at the command prompt. 5. See the acedb website for more information about acedb and using xace. ____________ END _____________