Difference between revisions of "Cosmids/YACs"
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This should help you [http://www.sanger.ac.uk/Projects/C_elegans/DOCS/vectors.shtml identify the vector] for the clone you are interested in.<br> | This should help you [http://www.sanger.ac.uk/Projects/C_elegans/DOCS/vectors.shtml identify the vector] for the clone you are interested in.<br> | ||
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+ | [http://www.ncbi.nlm.nih.gov/nuccore/401849 pYAC4] [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=67379&Template=vectors Ordering] | ||
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'''The V* plasmids are no longer available.''' | '''The V* plasmids are no longer available.''' | ||
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+ | == References == | ||
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+ | YAC information can be found in this publication: | ||
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+ | [http://www.nature.com/doifinder/10.1038/335184a0 Nature. 1988 Sep 8;335(6186):184-6. Genome linking with yeast artificial chromosomes. Coulson A, Waterston R, Kiff J, Sulston J, Kohara Y.] | ||
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+ | [http://www.sciencemag.org/cgi/content/abstract/236/4803/806 science 1987 May 15;236(806-812) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. DT Burke, GF Carle, and MV Olson] |
Revision as of 13:04, 20 July 2010
Contents
C. elegans Cosmid and YAC Info.
Ordering
Please submit all requests for C.elegans genomic clones via this request form.
Details are then automatically forwarded to Anne Angell. Please do not send request directly to this address.
Please remember to include a FedEx / DHL Account number to which shipping cost can be charged.
Note that the suffixes to YAC names (e.g. Y73B3A, Y73B3B) indicate sequenced segments of the YAC (Y73B3 in this case). They do not exist as subclones. You will be sent the whole YAC.
Vector Sequences
If you want the actual sequence of the vectors used they are on the Sanger FTP site.
This should help you identify the vector for the clone you are interested in.
Clone ends within wormbase
The set of clone ends is dumped as part of the gff files:
http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/GFF_files.shtml
This is the source for the extents displayed in WormBase.
The caveat with this is that the 'true' end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished).
Nomenclature
There are seven different types of "Clone" objects in WormBase:
Type | Nomenclature |
Cosmid | A*, B*, C*, D*, F*,J*,K*,M*,R*,T*,W*,Z* |
Fosmid | H* WRM* |
YAC | Y* |
cDNA | yk*, EC*, EB*, OST*, CK*, EF*, CEE*, CEM*, CES*, CB*, CN*, cm* |
Plasmid: PCR clones | V*,EGAP* |
Other | telo clones, 1 BAC, plasmid |
Most cosmids, fosmids, YACs can be requested from Sanger, cDNA (yk*) from Dr. Yuji Kohara. The EGAP* plasmids can be obtained from MRC Geneservice.
The V* plasmids are no longer available.
References
YAC information can be found in this publication: