Difference between revisions of "RNAi curation"
From WormBaseWiki
Jump to navigationJump to search (New page: When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating “we did...) |
|||
(One intermediate revision by one other user not shown) | |||
Line 1: | Line 1: | ||
− | When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating | + | When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating ‚"we did RNAi against unc-22" is not enough information for proper curation. We need to unabigiously assign the sequence used in an experiment. In the above case we are left to guess if you used a genomic sequence for interference (partial or full gene) or a cDNA ‚ (partial, full length which isoform?. |
+ | |||
+ | |||
+ | [[Category:Curation]] | ||
+ | [[Category:User Guide]] |
Latest revision as of 18:32, 16 August 2010
When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating ‚"we did RNAi against unc-22" is not enough information for proper curation. We need to unabigiously assign the sequence used in an experiment. In the above case we are left to guess if you used a genomic sequence for interference (partial or full gene) or a cDNA ‚ (partial, full length which isoform?.