WormBase gene association file

Generating the WormBase gene association file

In the acedb user account on Tazendra at:/home/acedb/ranjana/GO:

1. Use ftp://ftp.sanger.ac.uk/pub/wormbase/releases/WS211/ONTOLOGY/ eg., to get the latest gene association file generated by WormBase EBI eg., gene_association.WS211.wb.ce.
2. Use 'grep IEA gene_association.WSXXX.wb.ce > gene_association.wb.electronic to separate the IEAs.
3. Grep WBPhenotype gene_association.WSXXX.wb.ce > gene_association.wb.rnai2go(to get i.e both Erich's earlier RNAi2GO ones and the new associations based on allele phenotypes that went into WormBase WS186).
4. copy the right go.go.<date> file from /home/acedb/ranjana/citace_upload/go_curation/go_dumper_files/ to this directory,change name to gene_association.wb.manual.
5. Cd to the /external_annots directory and download the latest GOA elegans file,for external annots (use 'wget ftp://ftp.ebi.ac.uk/pub/databases/GO/goa/proteomes/9.C_elegans.goa')
6. Run the ./wrapper.pl script, note this script generates both go.ace and go.go (gene association format file) in the go_dumper_files directory:
7. ./wrapper.pl gives the following output: filtering secondary GO IDs into .no_secondary filenames now and outputs NUMBER of ERRORS by COLUMN:
8. Run strip_errors_and_concatenate.pl, this script also generates the file genecounts that has gene, GO term numbers.

On your local machine: In the tmp directory on Maya: --scp file to Maya --removed 'NOT' annotations from mtm-9, vha-2, vha-3, hsp-60, hsp-12.3, hsp-12.6. (We do not take out NOT annotations anymore) --removed header from the middle of concatenated file in two places (on top of UniProt file too, search for 'gaf-version') and placed on top of file (correct minor mistake in header--space after the $ on one of the lines) --And move the following header from the middle of file to the top of file: !Version: $Revision: $ !Organism: Caenorhabditis elegans !date: $Date: $ !From: WormBase --Add these two lines at the bottom of header: !DataBase_Project_Name: WormBase WS215/WS216 !gaf-version: 2.0 --Remove the header 'gaf 2.0', from the top of the UniProt file --gzip file --Copy file to the tmp directory --CVS_RSH=ssh; export CVS_RSH at terminal to use the RSH program NOTE-Upload:Stopped using the -z option to during: remove 'z3' from 'qz3' cvs -q -d :ext:ranjana@ext.geneontology.org:/share/go/cvs checkout go/gene-associations/submission/gene_association.wb.gz --Replace old file with the new gene_association.wb.gz under go/gene-associations/submission/ --To check in the new file: cvs -q -d :ext:ranjana@ext.geneontology.org:/share/go/cvs commit go/gene-associations/submission/gene_association.wb.gz new revision: 1.103; previous revision: 1.102

--Checkout README and modify to reflect the version change and any other changes: --cvs -q -d :ext:ranjana@ext.geneontology.org:/share/go/cvs checkout go/gene-associations/readme/WormBase.README Changes:Updated only for release numbers --For manual annotations column 2(DB_Object_ID) all converted into WBGene IDs, i.e WBGeneXXXXXXXX, from Dec, 2009 upload. --Commit new README (this time modified for only WS numbers): --cvs -q -d :ext:ranjana@ext.geneontology.org:/share/go/cvs commit go/gene-associations/readme/WormBase.README new revision: 1.54; previous revision: 1.53 (noted only version changes)