WormBase-Caltech Weekly Calls

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August 3, 2017

WB grant

• Progress (maintenance stuff) can be written as of last grant cycle
• Current projects and report the steady state

CRISPR alleles

• Some authors not reporting CRISPR alleles with standard nomenclature
• Some in Paul's lab creating same knockin with same name (should be distinct). Mary Ann to update nomenclature guidelines.
• Some (e.g. Bruce Bowerman) knock-in GFP in frame and use RNAi against GFP to knockdown endogenous gene
  • RNAi mapping pipeline not setup to handle this
  • Could maybe use endogenous sequence near insertion site (not ideal, for off-target effects)
  • Maybe best, once new ?Phenotype_experiment model is in place, to just refer to perturbed gene

Ontology Browser

• Has been less stable lately; Raymond has needed to restart machine
• Being served from Dell server (in Braun building)