New release of WormBase WS196, Wormpep196 and Wormrna196 Fri Oct 31 16:00:42 GMT 2008
WS196 was built by Mary Ann and Anthony
This directory includes: i) database.WS196.*.tar.gz - compressed data for new release ii) models.wrm.WS196 - the latest database schema (also in above database files) iii) CHROMOSOMES/subdir - contains 3 files (DNA, GFF & AGP per chromosome) iv) WS196-WS195.dbcomp - log file reporting difference from last release v) wormpep196.tar.gz - full Wormpep distribution corresponding to WS196 vi) wormrna196.tar.gz - latest WormRNA release containing non-coding RNA's in the genome vii) confirmed_genes.WS196.gz - DNA sequences of all genes confirmed by EST &/or cDNA viii) cDNA2orf.WS196.gz - Latest set of ORF connections to each cDNA (EST, OST, mRNA) ix) gene_interpolated_map_positions.WS196.gz - Interpolated map positions for each coding/RNA gene x) clone_interpolated_map_positions.WS196.gz - Interpolated map positions for each clone xi) best_blastp_hits.WS196.gz - for each C. elegans WormPep protein, lists Best blastp match to
human, fly, yeast, C. briggsae, and SwissProt & TrEMBL proteins.
xii) best_blastp_hits_brigprot.WS196.gz - for each C. briggsae protein, lists Best blastp match to
human, fly, yeast, C. elegans, and SwissProt & TrEMBL proteins.
xiii) geneIDs.WS196.gz - list of all current gene identifiers with CGC & molecular names (when known) xiv) PCR_product2gene.WS196.gz - Mappings between PCR products and overlapping Genes
Release notes on the web:
Genome sequence composition:
WS196 WS195 change
a 32365950 32365950 +0 c 17779889 17779889 +0 g 17756041 17756041 +0 t 32365753 32365753 +0 n 0 0 +0 - 0 0 +0
Total 100267633 100267633 +0
There are no changes to the chromosome sequences in this release.
Gene data set (Live C.elegans genes 29872)
Molecular_info 28155 (94.3%) Concise_description 5447 (18.2%) Reference 13254 (44.4%) WormBase_approved Gene name 15636 (52.3%) RNAi_result 20799 (69.6%) Microarray_results 20438 (68.4%) SAGE_transcript 18838 (63.1%)
Wormpep data set:
There are 20191 CDS in autoace, 23902 when counting 3711 alternate splice forms.
The 23902 sequences contain 10,518,004 base pairs in total.
Modified entries 66 Deleted entries 25 New entries 21 Reappeared entries 0
Net change -4
Status of entries: Confidence level of prediction (based on the amount of transcript evidence)
Confirmed 8517 (35.6%) Every base of every exon has transcription evidence (mRNA, EST etc.) Partially_confirmed 10985 (46.0%) Some, but not all exon bases are covered by transcript evidence Predicted 4400 (18.4%) No transcriptional evidence at all
Status of entries: Protein Accessions
UniProtKB accessions 23768 (99.4%)
Status of entries: Protein_ID's in EMBL
Protein_id 23772 (99.5%)
Gene <-> CDS,Transcript,Pseudogene connections
Caenorhabditis elegans entries with WormBase-approved Gene name 13967
GeneModel correction progress WS195 -> WS196
Confirmed introns not in a CDS gene model;
+---------+--------+ | Introns | Change | +---------+--------+ Cambridge | 40 | 13 | St Louis | 118 | 15 | +---------+--------+
Members of known repeat families that overlap predicted exons;
+---------+--------+ | Repeats | Change | +---------+--------+ Cambridge | 6 | 0 | St Louis | 6 | 0 | +---------+--------+
Synchronisation with GenBank / EMBL:
No synchronisation issues
There are no gaps remaining in the genome sequence
For more info mail email@example.com -===================================================================================-
This release includes the C. brenneri genome for the first time. Orthologies to the other species in WormBase have been determined and Gene names transferred from C. elegans where appropriate.
There has been some manual correction of the C. briggsae nGASP gene predictions.
Precalculated protein alignments are now made for each main WormBase species. This is available on the Sanger FTP site ftp.sanger.ac.uk/pub2/wormbase/live_release/wormpep_clw.sql.bz2 . For info contact WormBase.
Genome sequence updates:
Due to the amounts of data and time pressures to get C. brenneri in this release we didn't have time to load all of the blast hits for every species. They are still available in the GFF files and so visible in the genome browser but not via querying the acedb database.
Proposed Changes / Forthcoming Data:
added Person to #Affiliation
Quick installation guide for UNIX/Linux systems
1. Create a new directory to contain your copy of WormBase, e.g. /users/yourname/wormbase
2. Unpack and untar all of the database.*.tar.gz files into this directory. You will need approximately 2-3 Gb of disk space.
3. Obtain and install a suitable acedb binary for your system (available from www.acedb.org).
4. Use the acedb 'xace' program to open your database, e.g. type 'xace /users/yourname/wormbase' at the command prompt.
5. See the acedb website for more information about acedb and using xace.
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