UserGuide:How to Obtain Reagents
- How do I request a strain?
In the Strain page, there is tag called "Location", which means the location of the strain, usually, the location is at CGC, or a two-character name standing for a laboratory. CGC (C. elegans Genetics Center) has most of the strains in stock and they send out/receive strains to/from C. elegans Labs. Please view the following page for details on how to obtain strains: http://www.cbs.umn.edu/CGC/Strains/Changes.htm
If the strain is not available in CGC, you have to contact directly with the Lab, whose information can be directly accessed from "Location" data field. Please remind them also to send it to CGC for the convenience of other researchers in the future.
- How to obtain strains that contain transposon insertions? Who do I contact?
The best way to find strains that carry transposon insertions is to first identify those strains in WormBase, then contact authors directly.
Assuming you are looking for insertions within a specific region, you can browse for insertions in your region of interest using the Genome Browser (you'll need to first check "transposons" under the display settings).
After finding a transposon insertion, clicking on it will display it's laboratory of origin, strains carrying the insertion, etc. Some of these strains may be available directly from the CGC. Alternatively, you may wish to contact the lab of origin for its availablity.
- How do I obtain C.elegans cosmids?
As from January 1st 2003, please send all requests for C.elegans genomic clones to Audrey Fraser (firstname.lastname@example.org) and cc to Ratna Shownkeen (email@example.com). Please remember to include a FedEx/DHL Account number to which we can charge the shipping cost.
On a related matter, please note that the suffixes to YAC names (e.g. Y73B3A, Y73B3B) indicate sequenced segments of the YAC (Y73B3 in this case). They do not exist as subclones. You will be sent the whole YAC.
- How do I obtain C.elegans fosmid library?
Please contact with Geneservice Ltd; a UK based reagent and service provider. They distribute many genomic libraries worldwide. More specifically, they have exclusive distribution rights to Julie Ahringers' C.elegans RNAi feeding library. Here is the link for their distribution of Don Moerman's C.elegans fosmid library. http://www.geneservice.co.uk/products/clones/Celegans_Fos.jsp
- How do I get a YK cDNA clone?
Please send emails to Dr. Yuji Kohara (firstname.lastname@example.org).
- How do I obtain knockout strains?
-- Mary Ann Tuli