Difference between revisions of "Texpresso/Author/Curator interim form"

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| align="center" style="background:#f0f0f0;"|'''Data type Section'''
 
| align="center" style="background:#f0f0f0;"|'''Data type Section'''
 
| align="center" style="background:#f0f0f0;"|'''Data type'''
 
| align="center" style="background:#f0f0f0;"|'''Data type'''
| align="center" style="background:#f0f0f0;"|'''''Old curation form name''; PGdb name'''
+
| align="center" style="background:#f0f0f0;"|'''Old curation form name'''
 +
| align="center" style="background:#f0f0f0;"|'''PGdb name'''
 
| align="center" style="background:#f0f0f0;"|'''Description for author'''
 
| align="center" style="background:#f0f0f0;"|'''Description for author'''
 
| align="center" style="background:#f0f0f0;"|'''Curator Flagged'''
 
| align="center" style="background:#f0f0f0;"|'''Curator Flagged'''
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|||GENE IDENTIFICATION AND MAPPING:
 
|||GENE IDENTIFICATION AND MAPPING:
 
|-
 
|-
|||||'''''C. elegans'''''|| '''new field''' = celegans||Please indicate if ''C. elegans'' isolates other than Bristol are discussed in this paper|||
+
|||||'''''C. elegans'''''|| '''new field'''|| celegans||Please indicate if ''C. elegans'' isolates other than Bristol are discussed in this paper|||
 
|-
 
|-
|||||'''Nematodes other than ''C. elegans'''''||'''new field''' = nematodes||Please indicate if data is presented for any species other than ''C. elegans'', e.g., ''C. briggsae'', ''Pristionchus pacificus'', ''Brugia malayi'', etc.||
+
|||||'''Nematodes other than ''C. elegans'''''||'''new field'''||nematodes||Please indicate if data is presented for any species other than ''C. elegans'', e.g., ''C. briggsae'', ''Pristionchus pacificus'', ''Brugia malayi'', etc.||
 
|-
 
|-
|||||'''Newly cloned gene''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=genesymbol (flagged-done)]||''Gene Symbol'' ; genesymbol ||Please indicate if your paper presents a new symbol for a known locus or the name of a newly defined locus.||genenames@wormbase.org, vanauken@its.caltech.edu  
+
|||||'''Newly cloned gene''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=genesymbol (flagged-done)]||''Gene Symbol''|| genesymbol ||Please indicate if your paper presents a new symbol for a known locus or the name of a newly defined locus.||genenames@wormbase.org, vanauken@its.caltech.edu  
 
|-
 
|-
 
|in progress||||'''Newly created allele'''||''Extract allele'' ; extractedvariation||Please indicate if your paper reports the identification of any new alleles.||
 
|in progress||||'''Newly created allele'''||''Extract allele'' ; extractedvariation||Please indicate if your paper reports the identification of any new alleles.||
 
|-
 
|-
|||||'''Genetic mapping data''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=mappingdata (flagged)]||''mapping data'' ; mappingdata||Please indicated if your paper contains 3-factor interval mapping data, i.e., genetic data only, including with Df or Dp data but no SNP interval mapping.||genenames@wormbase.org  
+
|||||'''Genetic mapping data''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=mappingdata (flagged)]||''mapping data''||mappingdata||Please indicated if your paper contains 3-factor interval mapping data, i.e., genetic data only, including with Df or Dp data but no SNP interval mapping.||genenames@wormbase.org  
 
|-
 
|-
 
|||GENE FUNCTION:
 
|||GENE FUNCTION:
 
|-
 
|-
|||||'''Gene function''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=genefunction (flagged-done)]||''Gene function'' ; genefunction||Please indicate if your paper discusses a new function for a known gene or a newly defined gene.||emsch@its.caltech.edu
+
|||||'''Gene function''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=genefunction (flagged-done)]||''Gene function''||genefunction||Please indicate if your paper discusses a new function for a known gene or a newly defined gene.||emsch@its.caltech.edu
 
|-
 
|-
|in progress||||'''Homolog of a human disease-associated gene''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=humandiseases (flagged)]||''Human Disease'' ; humandiseases ||Please indicate if genes discussed in your paper are a homolog/ortholog of a human disease gene.
+
|in progress||||'''Homolog of a human disease-associated gene''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=humandiseases (flagged)]||''Human Disease''|| humandiseases ||Please indicate if genes discussed in your paper are a homolog/ortholog of a human disease gene.
 
||ranjana@its.caltech.edu  
 
||ranjana@its.caltech.edu  
 
|-
 
|-
 
|||Phenotype Analysis based on:
 
|||Phenotype Analysis based on:
 
|-
 
|-
|||||'''Allele analysis''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=newmutant (flagged-done)]|| ''Mutant Phenotype''; newmutant ||Please indicate if any phenotype is reported for a mutant variation. ||emsch@its.caltech.edu, garys@its.caltech.edu, jolenef@its.caltech.edu  
+
|||||'''Allele analysis''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=newmutant (flagged-done)]|| ''Mutant Phenotype''|| newmutant ||Please indicate if any phenotype is reported for a mutant variation. ||emsch@its.caltech.edu, garys@its.caltech.edu, jolenef@its.caltech.edu  
 
|-
 
|-
||in progress||||'''Small-scale RNAi''' (less than 100 experiments reported) [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=rnai (flagged-done)]||''RNAi'' ; rnai||Please indicate if your paper reports gene knockdown phenotypes for less than 100 individual RNAi experiments.||garys@its.caltech.edu
+
||in progress||||'''Small-scale RNAi''' (less than 100 experiments reported) [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=rnai (flagged-done)]||''RNAi''|| rnai||Please indicate if your paper reports gene knockdown phenotypes for less than 100 individual RNAi experiments.||garys@its.caltech.edu
 
|-
 
|-
||||||'''Large-scale RNAi''' (less than 100 experiments reported) [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=lsrnai (flagged-done)]||''Large-Scale RNAi'' ; lsrnai||Please indicate if your paper reports gene knockdown phenotypes for more than 100 individual RNAi experiments.||raymond@its.caltech.edu
+
||||||'''Large-scale RNAi''' (less than 100 experiments reported) [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=lsrnai (flagged-done)]||''Large-Scale RNAi''|| lsrnai||Please indicate if your paper reports gene knockdown phenotypes for more than 100 individual RNAi experiments.||raymond@its.caltech.edu
 
|-
 
|-
|||||'''Overexpression''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=overexpression (flagged)]||''Overexpression'' ; overexpression||Please indicated if your paper reports an abnormal phenotype based on the overexpression of a gene or gene construct. E.g., ""...constitutively activated SCD-2(neu*) receptor caused 100% of animals to arrest in the first larval stage (L1)..." ||emsch@its.caltech.edu, garys@its.caltech.edu  
+
|||||'''Overexpression''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=overexpression (flagged)]||''Overexpression''|| overexpression||Please indicated if your paper reports an abnormal phenotype based on the overexpression of a gene or gene construct. E.g., ""...constitutively activated SCD-2(neu*) receptor caused 100% of animals to arrest in the first larval stage (L1)..." ||emsch@its.caltech.edu, garys@its.caltech.edu  
 
|-
 
|-
|in progress||||'''Chemicals''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=chemicals (flagged)]||''Chemicals'' ; chemicals ||Please indicate if the effects of small molecules, chemicals, or drugs were studied on worms. E.g., paraquat, butanone, benzaldehyde, aldicarb, etc. Mutagens used for the generation of mutant in genetic screens do not need to be indicated.|||
+
|in progress||||'''Chemicals''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=chemicals (flagged)]||''Chemicals''|| chemicals ||Please indicate if the effects of small molecules, chemicals, or drugs were studied on worms. E.g., paraquat, butanone, benzaldehyde, aldicarb, etc. Mutagens used for the generation of mutant in genetic screens do not need to be indicated.|||
 
|-
 
|-
 
|||INTERACTIONS:
 
|||INTERACTIONS:
 
|-
 
|-
 
+
|||||'''Genetic interactions''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=geneinteractions (flagged-done)]||''Gene interactions'' ||geneinteractions||Please indicate if your paper reports the analysis of more than one gene at a time, e.g. double, triple, etc. mutants, including experiments that included RNAi concurrent with other RNAi-treated worms or mutants||emsch@its.caltech.edu
 
+
|-
 +
|||||'''Functional complementation''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=functionalcomplementation (flagged)]||''Functional Complementation'' ||functionalcomplementation ||Please indicate if your paper reports functional redundancy between separate genes such as the rescue of ''gen-A'', by overexpression of ''gen-B'' or any other extragenic sequence.||
 +
|-
 +
|||||'''Gene product interactions''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=geneproduct (flagged-done)]||''Gene product interaction'' ||geneproduct ||Please indicate if your paper reports protein-protein, RNA-protein, DNA-protein interactions, including Y2H, etc.|| emsch@its.caltech.edu
 
|-
 
|-
 
|||GENE EXPRESSION AND FUNCTION:
 
|||GENE EXPRESSION AND FUNCTION:
 
|-
 
|-
|||||'''New expression pattern for a gene''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=expression (flagged-done)]||''expression pattern data'' ; otherexpression ||Please indicate if your paper reports new temporal or spatial (e.g. tissue, subcellular, etc) data on the pattern of expression of any gene in a wild-type background. You can include:  reporter gene analysis, antibody staining, ''In situ'' hybridization, RT-PCR, Western or Northern blot data.||wchen@its.caltech.edu, vanauken@its.caltech.edu
+
|||||'''New expression pattern for a gene''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=expression (flagged-done)]||''expression pattern data''|| otherexpression ||Please indicate if your paper reports new temporal or spatial (e.g. tissue, subcellular, etc) data on the pattern of expression of any gene in a wild-type background. You can include:  reporter gene analysis, antibody staining, ''In situ'' hybridization, RT-PCR, Western or Northern blot data.||wchen@its.caltech.edu, vanauken@its.caltech.edu
 
|-
 
|-
 
|||Tools used for obtaining an expression pattern for a new gene:
 
|||Tools used for obtaining an expression pattern for a new gene:
 
|-
 
|-
|in progress||||'''''C. elegans'' antibodies''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=antibody (flagged-done)]||''Extract Antibody'' ; antibodies ||Please indicate if your paper reports the use of new or used antibodies created by your lab or someone else's lab; do not check this box if antibodies used were commercially bought.||wchen@its.caltech.edu  
+
|in progress||||'''''C. elegans'' antibodies''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=antibody (flagged-done)]||''Extract Antibody''|| antibodies ||Please indicate if your paper reports the use of new or used antibodies created by your lab or someone else's lab; do not check this box if antibodies used were commercially bought.||wchen@its.caltech.edu  
 
|-
 
|-
|'''DONE'''||||'''Integrated transgenes''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=transgene (flagged-done)] ||''Transgene'' ; transgene||Please indicate if integrated transgenes are used in this paper. If the transgene does not have a canonical name, please list it in the "Add Information text area" ||wchen@its.caltech.edu  
+
|'''DONE'''||||'''Integrated transgenes''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=transgene (flagged-done)] ||''Transgene''||transgene||Please indicate if integrated transgenes are used in this paper. If the transgene does not have a canonical name, please list it in the "Add Information text area" ||wchen@its.caltech.edu  
 
|-
 
|-
|||||'''Cell/Tissue reporters''' ||''Marker'' ; marker||Please indicate if reporters (integrated transgenes or antibodies) were used to mark certain tissues,  subcellular structures or life stages, etc. as a reference point to assay gene function or location.||wchen@its.caltech.edu, vanauken@its.caltech.edu  
+
|||||'''Cell or tissue reporters''' ||''Marker''|| marker||Please indicate if reporters (integrated transgenes or antibodies) were used to mark certain tissues,  subcellular structures or life stages, etc. as a reference point to assay gene function or location.||wchen@its.caltech.edu, vanauken@its.caltech.edu  
 
|-
 
|-
 
|||Regulation of gene expression patterns:
 
|||Regulation of gene expression patterns:
 
|-
 
|-
|in progress||||'''Gene expression altered by genetic background or other treatment'''  [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=generegulation (flagged-done)]||''Gene regulation on expression level'' ; generegulation ||Please indicate if your paper reports changes or lack of changes in gene expression levels or patterns due to genetic background, exposure to chemicals or temperature or any other experimental treatment.||xdwang@its.caltech.edu  
+
|in progress||||'''Alterations in gene expression by genetic background or other treatment'''  [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=generegulation (flagged-done)]||''Gene regulation on expression level'' || generegulation ||Please indicate if your paper reports changes or lack of changes in gene expression levels or patterns due to genetic background, exposure to chemicals or temperature or any other experimental treatment.||xdwang@its.caltech.edu  
 
|-
 
|-
|||||'''Regulatory sequence features'''  [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=sequencefeatures (flagged)]||''Sequence features'' ; sequencefeatures ||Please indicate if your paper reports any gene expression regulatory elements such as DNA/RNA elements required for gene expression,  promoters, introns, UTR's, DNA binding sites, etc.||xdwang@its.caltech.edu, worm-bug@sanger.ac.uk, stlouis@wormbase.org  
+
|||||'''Regulatory sequence features'''  [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=sequencefeatures (flagged)]||''Sequence features'' ||sequencefeatures ||Please indicate if your paper reports any gene expression regulatory elements such as DNA/RNA elements required for gene expression,  promoters, introns, UTR's, DNA binding sites, etc.||xdwang@its.caltech.edu, worm-bug@sanger.ac.uk, stlouis@wormbase.org  
 
|-
 
|-
|||||'''Position frequency matrix (PFM)''' or '''Postition weight matrix (PWM)'''||'''new field''' = matrices||Please indicate if your paper reports PFMs or PWMs that are typically used to define regulatory sites in genomic DNA (e.g., bound by transcription factors) or mRNA (e.g., bound by translational factors or miRNA). PFMs define simple nucleotide frequencies, while PWMs are scaled logarithmically against a background frequency.||xdwang@its.caltech.edu, emsch@its.caltech.edu
+
|||||'''Position frequency matrix (PFM)''' or '''Position weight matrix (PWM)'''||'''new field''' || matrices||Please indicate if your paper reports PFMs or PWMs that are typically used to define regulatory sites in genomic DNA (e.g., bound by transcription factors) or mRNA (e.g., bound by translational factors or miRNA). PFMs define simple nucleotide frequencies, while PWMs are scaled logarithmically against a background frequency.||xdwang@its.caltech.edu, emsch@its.caltech.edu
 
|-
 
|-
|||||'''Mosaic analysis''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=mosaic (flagged-done)]||''Mosaic analysis'' ; mosaic ||Please indicate if your paper reports cell specific gene function based on mosaic analysis, e.g. extra-chromosomal transgene loss in a particular cell lineage leads to loss of mutant rescue, etc. ||raymond@its.caltech.edu  
+
|||||'''Mosaic analysis''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=mosaic (flagged-done)]||''Mosaic analysis''||mosaic ||Please indicate if your paper reports cell specific gene function based on mosaic analysis, e.g. extra-chromosomal transgene loss in a particular cell lineage leads to loss of mutant rescue, etc. ||raymond@its.caltech.edu  
 
|-
 
|-
||||| '''Tissue or cell site of action''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=site (flagged-done)]||''Site of action'' ; site ||Please indicate if your paper reports anatomy(tissue/cell)-specific expression function for a gene.||raymond@its.caltech.edu  
+
||||| '''Tissue or cell site of action''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=site (flagged-done)]||''Site of action''|| site ||Please indicate if your paper reports anatomy(tissue/cell)-specific expression function for a gene.||raymond@its.caltech.edu  
 
|-
 
|-
|||||'''Developmental time of action'''||'''new field''' = timeofaction||Please indicate if your paper reports a temporal requirement for gene function.||raymond@its.caltech.edu
+
|||||'''Developmental time of action'''||'''new field''' || timeofaction||Please indicate if your paper reports a temporal requirement for gene function.||raymond@its.caltech.edu
 
|-
 
|-
|||||'''Microarray'''[http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=microarray (flagged-done)]||''Microarray'' ; microarray ||Please indicate if your paper reports any microarray data.||wchen@its.caltech.edu  
+
|||||'''Microarray'''[http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=microarray (flagged-done)]||''Microarray''|| microarray ||Please indicate if your paper reports any microarray data.||wchen@its.caltech.edu  
 
|-
 
|-
 
|||PROTEIN FUNCTION AND STRUCTURE:
 
|||PROTEIN FUNCTION AND STRUCTURE:
 
|-
 
|-
|||||'''Protein Analysis ''in vitro'''''[http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=invitro (flagged)]||in vitro ''Protein analysis''; invitro ||Please indicate if your paper reports any ''in vitro'' protein analysis including such things as kinase assays, agonist pharmacological studies, ''in vitro reconstitution studies, etc.||  
+
|||||'''Protein analysis ''in vitro'''''[http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=invitro (flagged)]||in vitro ''Protein analysis''|| invitro ||Please indicate if your paper reports any ''in vitro'' protein analysis including such things as kinase assays, agonist pharmacological studies, ''in vitro reconstitution studies, etc.||
 +
|-
 +
|||||'''Domain analysis'''||'''new field''' || domainanalysis ''note, should be populated with information previously in "structureinformation"''||Please indicate if your paper reports on a function of a particular domain within a protein.||
 +
|-
 +
|||||'''Covalent modification''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=covalent (flagged)]||''Covalent modification'' || covalent|| Please indicate if your paper reports on post-translational modifications as assayed by mutagenesis or ''in vitro'' analysis||
 +
|-
 +
|||||'''Structural information'''[http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=structureinformation (flagged)]||''Structure information''|| structureinformation||Please indicate if your paper reports NMR or X-ray crystallographic information.||  
 
|-
 
|-
|||||'''Domain Analysis'''||'''new field''' = domainanalysis||Please indicate if your paper reports on a function of a particular domain within a protein.||
+
|||||'''Mass spectrometry''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=massspec (flagged-done)]||''Mass Spec''|| massspec||Please indicate if your paper reports data from any mass spec analysis such as, LCMS, COSY, HRMS, etc.||gw3@sanger.ac.uk, worm-bug@sanger.ac.uk 
 
|-
 
|-
|||||'''Covalent Modification''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=covalent (flagged)]||''Covalent modification'' ; covalent|| Please indicate if your paper reports on post-translational modifications as assayed by mutagenesis or ''in vitro'' analysis||
+
|||GENOME SEQUENCE DATA:
 
|-
 
|-
|||||'''Structural Information '''Structural information''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=structureinformation (flagged)]: ||NMR structure, functional domain info for a protein ||''"removal of the first 50aa causes mislocalization of the protein"''; ''"fig.1, fig.8"''; ''"yes, in paper and supplemental materials"''; ''"FIGURE 5. The let-7 sequence is required for formation of the M1 and M2 complexes."''; ''"functional domains of gld-2, fig 3"''; ''"Fig. 3. Comparison of RDE-4 binding properties to variants lacking, or with mutations in, dsRBM1."'' ||  
+
|||||'''Gene structure correction''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=structurecorrectionsanger (flagged)]||''Sanger Gene Structure Correction'' and ''St. Louis Gene Structure Correction''|| structurecorrectionsanger (this use to be two different fields)||Please indicate if your paper reports a gene structure that is different from the one in WormBase, e.g.,  different splice-site, SL1 instead of SL2, etc.||worm-bug@sanger.ac.uk
 
|-
 
|-
||| Functional Complementation '''Functional Complementation''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=functionalcomplementation (flagged)]: ||Functional redundancy, rescue by overexpression of extragenic sequence ||''"exc-2 is rescued by exc-9 overexpression"''; ''"...meg-2 functions redundantly with meg-1. Each transgene can rescue the sterility of meg-1(vr10) or meg-1(vr11)... The partial rescue of meg-1 mutants by GFPTMEG-2 suggests that extra copies of MEG-2 can compensate for the absence of MEG-1, implying that the overall level of MEG-1 and MEG-2 is important for proper germline development."''; ''"In transformation experiments, we identified a cosmid, C26G6, which could rescue the egl-32 phenotype. Subclones of this cosmid containing the predicted open reading frame (ORF) T08G11.2 can also rescue egl-32 (Figures 3a & b). Several lines of evidence, however, suggest that egl-32 does not encode T08G11.2, but rather that they are interacting loci"''||  
+
|||||'''Sequencing mutant alleles''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=sequencechange (flagged)]||''Sequence change''||sequencechange ||Please indicate if your paper reports new sequence data for any mutation.||genenames@wormbase.org
 
|-
 
|-
 
+
|||||'''New SNPs''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=newsnp (flagged)]||''Extract New SNP''||newsnp||Please indicate if your paper reports a new SNP, i.e. it does not already exist in WormBase.||dblasiar@watson.wustl.edu, tbieri@watson.wustl.edu
 
 
||| Covalent Modification '''Covalent modification''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=covalent (flagged)]: ||phosphorylation site is studies via mutagenesis and in vitro assay ||''"Analysis of cDNA sequences derived from the eri-7 pre-mRNA stabilized in rnp-5 RNAi-treated animals revealed adenosine to guanosine transitions at four positions located within the direct repeat (Fig. 2d). These transitions are indicative of adenosine to inosine editing of the eri-7 59-UTR by an adenosine deaminase (ADAR)24"''
 
 
|-
 
|-
||| Non-N2_phenotype : ||Phenotypes of strains/non-C. elegans||||  
+
|retired? ||||?|| Extract SNP verified by St. Louis [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=stlouissnp (flagged)]||''Extract SNP verified by St.Louis''||''"fig.2: two SNPs mentioned"''; ''any SNP mentioned''; ''"For nekl-1, cDNA clones indicated that the predicted gene annotation was incorrect."'' ||dblasiar@watson.wustl.edu, tbieri@watson.wustl.edu
 
|-
 
|-
||| Sequence Change '''Sequencing mutant alleles''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=sequencechange (flagged)]: ||Mutation was sequenced ||''"the hcf- 1(pk924) mutant has a large deletion that should result in a frame shift leading to an early stop codon, and likely represents a null mutant (Figure S1) [35]."''; ''"table 2: unnamed mutations"''; ''"FIGURE 5. Predicted structure of the glo-3 gene and encoded protein. (A) The structure of the glo-3 gene and the location and phenotypic class of mutations are shown"''; ''"lin-15 mutation p.834"'' ||genenames@wormbase.org
+
|||CELL DATA:
 
|-
 
|-
||| Gene Interaction and Epistasis '''Genetic interactions''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=geneinteractions (flagged-done)]: ||analysis of more than one gene at a time, e.g. double, triple, etc. mutants, RNAi concurrent with other RNAi-treated worms or mutants||''"e.g. daf-16(mu86) suppresses daf-2(e1370), daf-16(RNAi) suppresses daf-2(RNAi)"''; ''"hcf-1, daf-16'';''daf-18, smg-1"''; ''"eri-6, eri-7"''; ''"gene interactions large-scale, table"''; ''"Epistasis analysis in figure 2 and table 2"'' ||emsch@its.caltech.edu  
+
|||||'''Ablation data''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=ablationdata (flagged-done)]||''Ablation data''||ablationdata ||Please indicate if your paper reports any assay involving any cell or anatomical unit being ablated by laser or by other means (e.g. by expressing a cell-toxic protein).||raymond@its.caltech.edu  
 
|-
 
|-
||| Gene Product Interaction '''Gene product interactions''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=geneproduct (flagged-done)]: ||protein-protein, RNA-protein, DNA-protein interactions, Y2H, etc., ||''"Table S4. PDZ domain specificity prediction"''; ''"Figure 5. Loss of hcf-1 Promotes the DAF-16 transcriptional Regulation of Several Target Genes...Figure 6. HCF-1 Forms a Protein Complex with DAF-16 in C. elegans..."''; ''"Interestingly, the eri-6/7 transsplicing reporter is more highly and broadly expressed when RNAi is attenuated by inactivation of the Argonaute gene rde-1 (Supplementary Fig. 12), suggesting that the eri-6/7 is a target of RNAi..."''; ''"Fig. 1. Identification of genes that regulate clec-85::gfp expression"'' || emsch@its.caltech.edu  
+
|||||'''Cell function''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=cellfunction (flagged-done)]||''Cell function''||cellfunction||Please indicate if your paper reports a function for any anatomical part (e.g., cell, tissue, etc.), which has not been indicated elsewhere on this form.||raymond@its.caltech.edu  
 
|-
 
|-
||| Sanger Gene Structure Correction '''Gene structure correction''' - need to make sure Sanger is responsible for the clone [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=structurecorrectionsanger (flagged)]: ||Gene Structure Correction (Gene Structure is different from the one in Wormbase: e.g. different splice-site, SL1 instead of SL2, etc.)||''"...JA:F59F5.2 targets two predicted genes, F59F5.2 and F59F5.8. Two previously isolated cDNAs, yk1328a05 and yk571h2, suggested that F59F5.2 and F59F5.8 are a single gene that is alternatively spliced to produce two transcripts. We independently isolated F59F5.2/.8 cDNAs from both adult and embryonic stages, supporting the conclusion that F59F5.2 and F59F5.8 are a single gene (see MATERIALS AND METHODS). We refer to the long F59F5.2/.8 transcript as glo-3 and the F59F5.2 transcript as glo-3 short (Figure 5A)."'' ||worm-bug@sanger.ac.uk
+
|||IN SILICO DATA:
 
|-
 
|-
||| St. Louis Gene Structure Correction '''Gene structure correction''' - need to make sure StLouis is responsible for the clone [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=structurecorrectionstlouis (flagged)]: ||Gene Structure Correction (Gene Structure is different from the one in Wormbase: e.g. different splice-site, SL1 instead of SL2, etc.)||''"C41D11.1 and C41D11.7 fig.1"''; ''"We confirmed two of three forms by isolation of cDNAs (gcy-28.a and gcy-28.c, corresponding to T01A4.1a and T01A4.1c, respectively) and identified a fourth splice form, T01A4.1d (gcy-28.d), which fuses the upstream predicted gene T01A4.2 to T01A4.1 (Figure 4A)."''; ''"page 3: By contrast, 235 of the indels occurred in regions with no perfectly aligning Solexa read, suggesting a possible error in the C. elegans reference genome,"''||wormticket@watson.wustl.edu
+
||||||'''Phylogenetic data'''||'''new field'''||phylogenetic||Please indicate if your paper reports any phylogenetic analysis.||
 
|-
 
|-
 
+
||||||'''Other bioinformatics analysis'''||'''new field'''||othersilico||Please indicate if your paper reports any bioinformatic data not indicated anywhere else on this form.||
||| Mass Spec '''Mass spectrometry''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=massspec (flagged-done)]: ||key words: LCMS, COSY, mass spec, HRMS||||gw3@sanger.ac.uk, worm-bug@sanger.ac.uk 
 
 
|-
 
|-
||| Cell/Anatomy Function '''Cell function''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=cellfunction (flagged-done)]: ||Function of any anatomical part (e.g. cell) is mentioned that has not been flagged already for mosaic analysis, site of action, or ablation data||''"Figure 3 and Supp table 2 have information on predicted navigation circuit."'' ||raymond@its.caltech.edu
+
|||OTHER:
 
|-
 
|-
||| Ablation Data '''Ablation data''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=ablationdata (flagged-done)]: ||cell or anatomical unit was ablated using a laser or by other means (e.g. by expressing a cell-toxic protein)||''"Killing AWCON in wild-type animals abolished the net migration toward butanone (Figure 3A; Wes and Bargmann, 2001"''||raymond@its.caltech.edu  
+
|||||'''Supplemental materials''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=supplemental (flagged-done)]||''Supplemental material''||supplemental||Please indicate if your paper has supplemental material.||qwang@its.caltech.edu  
 
|-
 
|-
||| Extract New SNP '''New SNPs''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=newsnp (flagged)]: ||Reagent: new SNP not already in WB||''"page 3: By contrast, 235 of the indels occurred in regions with no perfectly aligning Solexa read, suggesting a possible error in the C. elegans reference genome,"''||dblasiar@watson.wustl.edu, tbieri@watson.wustl.edu
+
|||||'''NONE of the aforementioned data types are in this research article'''||'''Comment'''||nocuratable||Please indicate if none of the above pertains to your paper. Feel free to list the data type most pertinent to your research paper in the "Add information" text area.||
 
|-
 
|-
|retired? || Extract SNP Verified by St. Louis [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=stlouissnp (flagged)]: ||Authors report reference genome is incorrect||''"fig.2: two SNPs mentioned"''; ''any SNP mentioned''; ''"For nekl-1, cDNA clones indicated that the predicted gene annotation was incorrect."'' ||dblasiar@watson.wustl.edu, tbieri@watson.wustl.edu
+
|||||'''Comment''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=comment (flagged)]||'''new field'''||comment||Please feel free to give us feedback on this form or on any other topic pertinent to how we can better extract data from your paper.||''this should go to someone''
 
|-
 
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||| Supplemental Material '''Supplemental materials''' [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fc&field=supplemental (flagged-done)]: ||Flag if supplemental material was not already downloaded. |||''yes''||qwang@its.caltech.edu
+
Left overs:
 +
|||||?||''Non-N2_phenotype''||?nematode?||used to be for gathering information on phenotypes of non-C. elegans nematodes||
 
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|-
 
 
||| Comment [http://tazendra.caltech.edu/~postgres/cgi-bin/curation_status.cgi?action=fnc&field=comment (flagged)]: ||''e.g. no curatable'';''review'';''the paper is used for functional annotations''||||''stays in Postgres''
 
 
|}
 
|}

Revision as of 01:58, 11 March 2009

[back]

New First Pass Curator form

"flagged" = WBPapers that have been flagged for that particular data type but not curated yet or not assessed for curation status yet; "flagged-done" = WBPapers that have been flagged and curated. These papers can be used as a source for verified curation flag examples.

Left overs:
Automation status Data type Section Data type Old curation form name PGdb name Description for author Curator Flagged
GENE IDENTIFICATION AND MAPPING:
C. elegans new field celegans Please indicate if C. elegans isolates other than Bristol are discussed in this paper
Nematodes other than C. elegans new field nematodes Please indicate if data is presented for any species other than C. elegans, e.g., C. briggsae, Pristionchus pacificus, Brugia malayi, etc.
Newly cloned gene (flagged-done) Gene Symbol genesymbol Please indicate if your paper presents a new symbol for a known locus or the name of a newly defined locus. genenames@wormbase.org, vanauken@its.caltech.edu
in progress Newly created allele Extract allele ; extractedvariation Please indicate if your paper reports the identification of any new alleles.
Genetic mapping data (flagged) mapping data mappingdata Please indicated if your paper contains 3-factor interval mapping data, i.e., genetic data only, including with Df or Dp data but no SNP interval mapping. genenames@wormbase.org
GENE FUNCTION:
Gene function (flagged-done) Gene function genefunction Please indicate if your paper discusses a new function for a known gene or a newly defined gene. emsch@its.caltech.edu
in progress Homolog of a human disease-associated gene (flagged) Human Disease humandiseases Please indicate if genes discussed in your paper are a homolog/ortholog of a human disease gene. ranjana@its.caltech.edu
Phenotype Analysis based on:
Allele analysis (flagged-done) Mutant Phenotype newmutant Please indicate if any phenotype is reported for a mutant variation. emsch@its.caltech.edu, garys@its.caltech.edu, jolenef@its.caltech.edu
in progress Small-scale RNAi (less than 100 experiments reported) (flagged-done) RNAi rnai Please indicate if your paper reports gene knockdown phenotypes for less than 100 individual RNAi experiments. garys@its.caltech.edu
Large-scale RNAi (less than 100 experiments reported) (flagged-done) Large-Scale RNAi lsrnai Please indicate if your paper reports gene knockdown phenotypes for more than 100 individual RNAi experiments. raymond@its.caltech.edu
Overexpression (flagged) Overexpression overexpression Please indicated if your paper reports an abnormal phenotype based on the overexpression of a gene or gene construct. E.g., ""...constitutively activated SCD-2(neu*) receptor caused 100% of animals to arrest in the first larval stage (L1)..." emsch@its.caltech.edu, garys@its.caltech.edu
in progress Chemicals (flagged) Chemicals chemicals Please indicate if the effects of small molecules, chemicals, or drugs were studied on worms. E.g., paraquat, butanone, benzaldehyde, aldicarb, etc. Mutagens used for the generation of mutant in genetic screens do not need to be indicated.
INTERACTIONS:
Genetic interactions (flagged-done) Gene interactions geneinteractions Please indicate if your paper reports the analysis of more than one gene at a time, e.g. double, triple, etc. mutants, including experiments that included RNAi concurrent with other RNAi-treated worms or mutants emsch@its.caltech.edu
Functional complementation (flagged) Functional Complementation functionalcomplementation Please indicate if your paper reports functional redundancy between separate genes such as the rescue of gen-A, by overexpression of gen-B or any other extragenic sequence.
Gene product interactions (flagged-done) Gene product interaction geneproduct Please indicate if your paper reports protein-protein, RNA-protein, DNA-protein interactions, including Y2H, etc. emsch@its.caltech.edu
GENE EXPRESSION AND FUNCTION:
New expression pattern for a gene (flagged-done) expression pattern data otherexpression Please indicate if your paper reports new temporal or spatial (e.g. tissue, subcellular, etc) data on the pattern of expression of any gene in a wild-type background. You can include: reporter gene analysis, antibody staining, In situ hybridization, RT-PCR, Western or Northern blot data. wchen@its.caltech.edu, vanauken@its.caltech.edu
Tools used for obtaining an expression pattern for a new gene:
in progress C. elegans antibodies (flagged-done) Extract Antibody antibodies Please indicate if your paper reports the use of new or used antibodies created by your lab or someone else's lab; do not check this box if antibodies used were commercially bought. wchen@its.caltech.edu
DONE Integrated transgenes (flagged-done) Transgene transgene Please indicate if integrated transgenes are used in this paper. If the transgene does not have a canonical name, please list it in the "Add Information text area" wchen@its.caltech.edu
Cell or tissue reporters Marker marker Please indicate if reporters (integrated transgenes or antibodies) were used to mark certain tissues, subcellular structures or life stages, etc. as a reference point to assay gene function or location. wchen@its.caltech.edu, vanauken@its.caltech.edu
Regulation of gene expression patterns:
in progress Alterations in gene expression by genetic background or other treatment (flagged-done) Gene regulation on expression level generegulation Please indicate if your paper reports changes or lack of changes in gene expression levels or patterns due to genetic background, exposure to chemicals or temperature or any other experimental treatment. xdwang@its.caltech.edu
Regulatory sequence features (flagged) Sequence features sequencefeatures Please indicate if your paper reports any gene expression regulatory elements such as DNA/RNA elements required for gene expression, promoters, introns, UTR's, DNA binding sites, etc. xdwang@its.caltech.edu, worm-bug@sanger.ac.uk, stlouis@wormbase.org
Position frequency matrix (PFM) or Position weight matrix (PWM) new field matrices Please indicate if your paper reports PFMs or PWMs that are typically used to define regulatory sites in genomic DNA (e.g., bound by transcription factors) or mRNA (e.g., bound by translational factors or miRNA). PFMs define simple nucleotide frequencies, while PWMs are scaled logarithmically against a background frequency. xdwang@its.caltech.edu, emsch@its.caltech.edu
Mosaic analysis (flagged-done) Mosaic analysis mosaic Please indicate if your paper reports cell specific gene function based on mosaic analysis, e.g. extra-chromosomal transgene loss in a particular cell lineage leads to loss of mutant rescue, etc. raymond@its.caltech.edu
Tissue or cell site of action (flagged-done) Site of action site Please indicate if your paper reports anatomy(tissue/cell)-specific expression function for a gene. raymond@its.caltech.edu
Developmental time of action new field timeofaction Please indicate if your paper reports a temporal requirement for gene function. raymond@its.caltech.edu
Microarray(flagged-done) Microarray microarray Please indicate if your paper reports any microarray data. wchen@its.caltech.edu
PROTEIN FUNCTION AND STRUCTURE:
Protein analysis in vitro(flagged) in vitro Protein analysis invitro Please indicate if your paper reports any in vitro protein analysis including such things as kinase assays, agonist pharmacological studies, in vitro reconstitution studies, etc.
Domain analysis new field domainanalysis note, should be populated with information previously in "structureinformation" Please indicate if your paper reports on a function of a particular domain within a protein.
Covalent modification (flagged) Covalent modification covalent Please indicate if your paper reports on post-translational modifications as assayed by mutagenesis or in vitro analysis
Structural information(flagged) Structure information structureinformation Please indicate if your paper reports NMR or X-ray crystallographic information.
Mass spectrometry (flagged-done) Mass Spec massspec Please indicate if your paper reports data from any mass spec analysis such as, LCMS, COSY, HRMS, etc. gw3@sanger.ac.uk, worm-bug@sanger.ac.uk
GENOME SEQUENCE DATA:
Gene structure correction (flagged) Sanger Gene Structure Correction and St. Louis Gene Structure Correction structurecorrectionsanger (this use to be two different fields) Please indicate if your paper reports a gene structure that is different from the one in WormBase, e.g., different splice-site, SL1 instead of SL2, etc. worm-bug@sanger.ac.uk
Sequencing mutant alleles (flagged) Sequence change sequencechange Please indicate if your paper reports new sequence data for any mutation. genenames@wormbase.org
New SNPs (flagged) Extract New SNP newsnp Please indicate if your paper reports a new SNP, i.e. it does not already exist in WormBase. dblasiar@watson.wustl.edu, tbieri@watson.wustl.edu
retired? ? Extract SNP verified by St. Louis (flagged) Extract SNP verified by St.Louis "fig.2: two SNPs mentioned"; any SNP mentioned; "For nekl-1, cDNA clones indicated that the predicted gene annotation was incorrect." dblasiar@watson.wustl.edu, tbieri@watson.wustl.edu
CELL DATA:
Ablation data (flagged-done) Ablation data ablationdata Please indicate if your paper reports any assay involving any cell or anatomical unit being ablated by laser or by other means (e.g. by expressing a cell-toxic protein). raymond@its.caltech.edu
Cell function (flagged-done) Cell function cellfunction Please indicate if your paper reports a function for any anatomical part (e.g., cell, tissue, etc.), which has not been indicated elsewhere on this form. raymond@its.caltech.edu
IN SILICO DATA:
Phylogenetic data new field phylogenetic Please indicate if your paper reports any phylogenetic analysis.
Other bioinformatics analysis new field othersilico Please indicate if your paper reports any bioinformatic data not indicated anywhere else on this form.
OTHER:
Supplemental materials (flagged-done) Supplemental material supplemental Please indicate if your paper has supplemental material. qwang@its.caltech.edu
NONE of the aforementioned data types are in this research article Comment nocuratable Please indicate if none of the above pertains to your paper. Feel free to list the data type most pertinent to your research paper in the "Add information" text area.
Comment (flagged) new field comment Please feel free to give us feedback on this form or on any other topic pertinent to how we can better extract data from your paper. this should go to someone
? Non-N2_phenotype ?nematode? used to be for gathering information on phenotypes of non-C. elegans nematodes