https://wiki.wormbase.org/index.php?title=Reporting_sequence_for_RNAi&feed=atom&action=historyReporting sequence for RNAi - Revision history2024-03-29T09:17:40ZRevision history for this page on the wikiMediaWiki 1.33.0https://wiki.wormbase.org/index.php?title=Reporting_sequence_for_RNAi&diff=6108&oldid=prevCgrove at 18:56, 16 August 20102010-08-16T18:56:14Z<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 18:56, 16 August 2010</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l16" >Line 16:</td>
<td colspan="2" class="diff-lineno">Line 16:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Please report the primer sequences used for amplification and template for amplification (e.g. Genomic DNA or specific clone)</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Please report the primer sequences used for amplification and template for amplification (e.g. Genomic DNA or specific clone)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:User Guide]]</ins></div></td></tr>
</table>Cgrovehttps://wiki.wormbase.org/index.php?title=Reporting_sequence_for_RNAi&diff=217&oldid=prevTharris: New page: In order to properly display the information gained from an RNAi experiment on the WormBase website, the curators need to be able to figure out the exact sequence used for interference by ...2007-09-24T15:34:59Z<p>New page: In order to properly display the information gained from an RNAi experiment on the WormBase website, the curators need to be able to figure out the exact sequence used for interference by ...</p>
<p><b>New page</b></p><div>In order to properly display the information gained from an RNAi experiment on the WormBase website, the curators need to be able to figure out the exact sequence used for interference by the researcher. The best ways to report the sequences in the materials and methods are the following:<br />
<br />
'''Use of Standard primer pairs to report sequence'''<br />
<br />
Reporting of specific primer pairs (e.g. sjj_ZK617.1 or cenix:155-g4 or mv_CAA33463) or library used (e.g. Ahringer) is sufficient.<br />
<br />
'''Use of Genomic Coordinates'''<br />
<br />
Reporting the genomic start and stop coordinates of the RNAi used is also sufficient. e.g. IV:11970667..12012891 or ZK1067:13703..22295 or MtDNA:4504..5613<br />
<br />
'''Use of cDNA/EST/OST Clone names'''<br />
<br />
Reporting the cDNA/EST/OST clone names (e.g. AF071375, yk275g9, OSTR215B4_1)<br />
<br />
'''Use of non-standard primer pairs to report sequence'''<br />
<br />
Please report the primer sequences used for amplification and template for amplification (e.g. Genomic DNA or specific clone)</div>Tharris