Difference between revisions of "Reporting sequence for RNAi"
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Latest revision as of 18:56, 16 August 2010
In order to properly display the information gained from an RNAi experiment on the WormBase website, the curators need to be able to figure out the exact sequence used for interference by the researcher. The best ways to report the sequences in the materials and methods are the following:
Use of Standard primer pairs to report sequence
Reporting of specific primer pairs (e.g. sjj_ZK617.1 or cenix:155-g4 or mv_CAA33463) or library used (e.g. Ahringer) is sufficient.
Use of Genomic Coordinates
Reporting the genomic start and stop coordinates of the RNAi used is also sufficient. e.g. IV:11970667..12012891 or ZK1067:13703..22295 or MtDNA:4504..5613
Use of cDNA/EST/OST Clone names
Reporting the cDNA/EST/OST clone names (e.g. AF071375, yk275g9, OSTR215B4_1)
Use of non-standard primer pairs to report sequence
Please report the primer sequences used for amplification and template for amplification (e.g. Genomic DNA or specific clone)