Difference between revisions of "RNAi curation"
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When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating ‚"we did RNAi against unc-22" is not enough information for proper curation. We need to unabigiously assign the sequence used in an experiment. In the above case we are left to guess if you used a genomic sequence for interference (partial or full gene) or a cDNA ‚ (partial, full length which isoform?. | When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating ‚"we did RNAi against unc-22" is not enough information for proper curation. We need to unabigiously assign the sequence used in an experiment. In the above case we are left to guess if you used a genomic sequence for interference (partial or full gene) or a cDNA ‚ (partial, full length which isoform?. | ||
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+ | [[Category:User Guide]] |
Latest revision as of 18:32, 16 August 2010
When an RNAi experiment is curated by WormBase, the experimental details are attached to the sequence of the interfering RNA, not directly to the gene name; therefore stating ‚"we did RNAi against unc-22" is not enough information for proper curation. We need to unabigiously assign the sequence used in an experiment. In the above case we are left to guess if you used a genomic sequence for interference (partial or full gene) or a cDNA ‚ (partial, full length which isoform?.