PMID:26412237
Figure in Paper | Evidence Sentence(s) | Gene or Protein | WPO Annotation | EQ Annotation | GO Annotation | Comments |
---|---|---|---|---|---|---|
Figure S1 | ULP-2, a member of the Ulp2-like branch of SUMO proteases (Mukhopadhyay and Dasso, 2007) shares 17% amino acid similarity with the mammalian SUMO proteases SENP6 and SENP7 (Figure S1). | ULP-2 | n/a | n/a | MF - GO:0016929 SUMO-specific protease activity
BP - GO:0016926 protein desumoylation EC = ISS with human SENP6 |
Note that WB and UniProtKB both only represent the larger isoform right now. |
Figure 1A-C, 1D and 1E | RT-PCR of embryonic mRNA identified two ulp-2 isoforms; ulp-2a, which is expressed in both the nucleus and cytosol, and the shorter isoform ulp-2b, which is expressed solely in the cytosol (Figures 1D and 1E). | UPL-2 | n/a | n/a | CC - GO:0005634 nucleus
CC - GO:0005829 cytosol EC = IDA |
Note that WB and UniProtKB both only represent the larger isoform right now. |
Figure 2, Figure S2 | Embryos in which ulp-2 expression was silenced by RNAi or gene mutation—ulp-2(tv380), which we generated by the CRISPR/Cas9 system (Friedland et al., 2013)—were arrested (29% and 63.4% embryonic arrest, respectively) during a developmental window from late gastrulation through epidermal morphogenesis. | ulp-2 | BP - GO:0009792 embryo development ending in birth or egg hatching
EC = IMP |
Note that BP - GO:0009790 embryo development is included in the 'do not manually annotate' subset of GO terms |
Existing GO Terms
GO:0007369 gastrulation
GO:0003381 epithelial cell morphogenesis involved in gastrulation
GO:0070587 regulation of cell-cell adhesion involved in gastrulation
GO:0016331 morphogenesis of embryonic epithelium
GO:0010172 embryonic body morphogenesis
GO:1904696 protein localization to cell-cell adherens junctions
GO:1904704 positive regulation of protein localization to cell-cell adherens junctions
GO:0016926 protein desumoylation
GO:0034334 adherens junction maintenance
GO:0071260 cellular response to mechanical stimulus
Possible New GO Terms
GO:new? ventral cleft closure?
GO:new? ventral epidermal enclosure?
GO:new? dorsal intercalation?
GO:new? embryonic elongation?
GO:new? regulation of cell-cell adhesion involved in morphogenesis of embryonic epithelium?
GO:new? positive regulation of protein localization to cell-cell adherens junctions via protein desumoylation?
Chris' allele-phenotype EQ-PATO annotations
anatomical structure morphogenesis (Figure 4A)
apical protein localization (Figure 6A-C)
developmental process (Figure 4B)
embryo development (Figure S2A,D, 3E, 4A)
embryo development, gastrulation (Figures 3A-3C, 3G, and 3H; Table S1; Movie S5)
gastrulation PATO: failure/non-functional (Figure S2A,D, 4A)
regulation of protein sumoylation (Figure 5F)
Chris' RNAi EQ-PATO annotations:
actin filament bundle assembly (Figure 7C-E)
apical protein localization (Figure 6)
body morphogenesis (Figure 4B, Movie S6)
cell morphogenesis (Figure 2E)
embryo development (Figure 2C,D,F, 4A, S2A,D)
digestive tract development (Figure S2F)
gastrulation (Figure 2C,S2D,F, 4A,B, Movie S6)
neuroblast migration (Figure S2E)
protein localization (Figure 6D)
regulation of protein sumoylation (Figure 5F)
catenin complex PATO: mislocalized (Figure 7A,B)
cell-cell contact zone (Figure 6D, S3D,E)