ModENCODE

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This is a summary of what we need to get out of modENCODE

5 main areas

The Transcriptome
Chromatin Function
Histone Variants
Regulatory Elements
The 3' UTRome

Datasets available from modmine:

title description DCCid design experimentDate publicReleaseDate embargoDate
3' UTR 454 sequencing pilot - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 992 transcript_identification_design Thu Nov 12 00:00:00 GMT 2009 Thu Nov 12 00:00:00 GMT 2009 Tue Aug 03 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L1 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2327 transcript_identification_design Thu Nov 12 00:00:00 GMT 2009 Thu Nov 12 00:00:00 GMT 2009 Fri Aug 06 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L1 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2455 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L2 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2328 transcript_identification_design Thu Nov 12 00:00:00 GMT 2009 Thu Nov 12 00:00:00 GMT 2009 Fri Aug 06 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L2 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2456 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L3 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2329 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Tue Aug 03 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L3 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2457 transcript_identification_design Tue Nov 03 00:00:00 GMT 2009 Tue Nov 03 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L4 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2330 transcript_identification_design Thu Nov 12 00:00:00 GMT 2009 Thu Nov 12 00:00:00 GMT 2009 Fri Aug 06 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - L4 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2458 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-11 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2334 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Tue Aug 03 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-11 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2464 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-2 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2335 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Tue Aug 03 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-2 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2461 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-7 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2336 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Tue Aug 03 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-7 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2462 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-9 - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2337 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Fri Aug 06 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - daf-9 - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2463 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - embryo - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2331 transcript_identification_design Thu Nov 12 00:00:00 GMT 2009 Thu Nov 12 00:00:00 GMT 2009 Fri Aug 06 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - embryo - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2465 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - hermaphrodite adult - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2332 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Tue Aug 03 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - hermaphrodite adult - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2460 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - male adult - alignments The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2333 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Fri Aug 06 01:00:00 BST 2010
3' UTR Staged 454 Sequencing - male adult - sequences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2459 transcript_identification_design Wed Nov 04 00:00:00 GMT 2009 Wed Nov 04 00:00:00 GMT 2009 Mon Jul 19 01:00:00 BST 2010
3' UTRome 454 sequencing & alignment The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2482 transcript_identification_design Mon Nov 16 00:00:00 GMT 2009 Mon Nov 16 00:00:00 GMT 2009 Mon Aug 09 01:00:00 BST 2010
3' UTRome Solexa sequencing & alignment The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2484 transcript_identification_design Mon Nov 16 00:00:00 GMT 2009 Mon Nov 16 00:00:00 GMT 2009 Mon Aug 09 01:00:00 BST 2010
3' UTRome sample pools The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2501 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Mon Aug 09 01:00:00 BST 2010
3'UTR 454 sequencing pilot - Level 1 The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 896 transcript_identification_design Tue Nov 03 00:00:00 GMT 2009 Tue Nov 03 00:00:00 GMT 2009 Fri Jun 04 01:00:00 BST 2010
3'UTRome CEUP1 sequences, alignments, annotation The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 515 transcript_identification_design Mon Nov 16 00:00:00 GMT 2009 Mon Nov 16 00:00:00 GMT 2009 Wed Aug 04 01:00:00 BST 2010
600_mM_Embryonic_Salt_Extracted_Chromatin We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2532 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
600_mM_Embryonic_Salt_Extracted_Chromatin_Pellet We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2533 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
80_mM_Embryonic_Salt_Extracted_Chromatin We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2531 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
Adult spe-9 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2850 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
Adult_Mononucleosomes We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2538 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
Ahringer_L3_Worm_Samples_1 The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2539 development_or_differentiation_design Fri Jul 10 01:00:00 BST 2009 Mon Aug 10 01:00:00 BST 2009 Sun Aug 29 01:00:00 BST 2010
Ahringer_L3_Worm_Samples_2 The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2540 development_or_differentiation_design Fri Jul 10 01:00:00 BST 2009 Mon Aug 10 01:00:00 BST 2009 Mon Aug 30 01:00:00 BST 2010
C. elegans Intron Identification set.20090106.2_3_2_2_4_3_3_2_2 This experiment identifies intron boundary coordinates in C. elegans
genomic sequence. Initially, we run genefinder to predict protein-coding
transcripts from the C. elegans chromosome sequences. We align
existing cDNA and EST sequences to the predicted transcript sequences
to confirm the transcript structure. Predicted splice junctions
unconfirmed by these alignments are tested for confirmation using
RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
use gene-specific/gene-specific, 5' RACE/gene-specific, and
gene-specific/3' RACE primer pairs. 448 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Sun Oct 25 01:00:00 BST 2009
C. elegans Intron Identification set.20090106.2_3_2_2_4_4_3_2_2 This experiment identifies intron boundary coordinates in C. elegans
genomic sequence. Initially, we run genefinder to predict protein-coding
transcripts from the C. elegans chromosome sequences. We align
existing cDNA and EST sequences to the predicted transcript sequences
to confirm the transcript structure. Predicted splice junctions
unconfirmed by these alignments are tested for confirmation using
RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
use gene-specific/gene-specific, 5' RACE/gene-specific, and
gene-specific/3' RACE primer pairs. 446 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Sun Oct 25 01:00:00 BST 2009
C. elegans Intron Identification set.20090106.2_3_2_2_5_3_3_2_2 This experiment identifies intron boundary coordinates in C. elegans
genomic sequence. Initially, we run genefinder to predict protein-coding
transcripts from the C. elegans chromosome sequences. We align
existing cDNA and EST sequences to the predicted transcript sequences
to confirm the transcript structure. Predicted splice junctions
unconfirmed by these alignments are tested for confirmation using
RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
use gene-specific/gene-specific, 5' RACE/gene-specific, and
gene-specific/3' RACE primer pairs. 447 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Sat Oct 31 00:00:00 GMT 2009
C. elegans Intron Identification.set.20090106.2_3_2_2_3_2_2_2_2 This experiment identifies intron boundary coordinates in C. elegans
genomic sequence. Initially, we run genefinder to predict protein-coding
transcripts from the C. elegans chromosome sequences. We align
existing cDNA and EST sequences to the predicted transcript sequences
to confirm the transcript structure. Predicted splice junctions
unconfirmed by these alignments are tested for confirmation using
RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
use gene-specific/gene-specific, 5' RACE/gene-specific, and
gene-specific/3' RACE primer pairs. 445 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Sun Oct 25 01:00:00 BST 2009
CBP-1_N2_MXEMB_(SDQ3582_CBP1_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2767 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Mon Feb 01 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
DPY-26_N2_Mixed_Embryos_(JL00003_DPY26_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 334 binding_site_identification_design Mon Aug 25 01:00:00 BST 2008 Mon Nov 30 00:00:00 GMT 2009 Mon Aug 23 01:00:00 BST 2010
DPY-27_N2_L4(JL00001_DPY27_N2_L4) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 630 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
DPY-27_N2_MXEMB_1A_(JL00001_DPY27_N2_MXEMB_1_A) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 578 binding_site_identification_design Fri Nov 27 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
DPY-27_N2_Mixed_Embryos (JL00001_DPY27_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 90 binding_site_identification_design Fri Dec 01 00:00:00 GMT 2006 Sun Feb 11 00:00:00 GMT 2007 Mon Aug 16 01:00:00 BST 2010
DPY-28_N2_MXEMB_(JL00012_DPY28_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 644 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
DPY27_11DH(I;X)_MXEMB(JL00001_DPY27_11DH_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 693 binding_site_identification_design Wed Feb 03 00:00:00 GMT 2010 Wed Feb 03 00:00:00 GMT 2010 Mon Aug 16 01:00:00 BST 2010
DPY27_YPT41(II;X)_MXEMB(JL00001_DPY27_YPT41_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 695 binding_site_identification_design Wed Feb 03 00:00:00 GMT 2010 Wed Feb 03 00:00:00 GMT 2010 Mon Aug 16 01:00:00 BST 2010
DPY27_YPT47(V;X)_MXEMB(JL00001_DPY27_YPT47_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 696 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Day0_spe-9 Solexa Raw Data SOAP-aligned We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2730 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
Day12_spe-9 Solexa Raw Data SOAP-aligned We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2734 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
Day12_spe-9_23dC_miRNA_expression_change We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2788 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Sat Nov 13 00:00:00 GMT 2010
Day5_spe-9 Solexa Raw Data SOAP-aligned We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2732 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
Day8_spe-9 Solexa Raw Data SOAP-aligned We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2733 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
Embryo_miRNA_expression_change We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2776 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Sat Nov 13 00:00:00 GMT 2010
Embryonic_Mononucleosomes We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2537 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
H3.3_350_mM_Salt_Extracted_Chromatin We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2535 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
H3.3_600_mM_Salt_Extracted_Chromatin We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2536 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
H3.3_80_mM_Salt_Extracted_Chromatin We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics. 2534 binding_site_identification_design Mon Dec 15 00:00:00 GMT 2008 Sun Nov 29 00:00:00 GMT 2009 Fri Aug 27 01:00:00 BST 2010
HCP-3_CENP-A_Mixed_Embryos_(OD00001_HCP3_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 194 binding_site_identification_design Wed Jul 02 01:00:00 BST 2008 Wed Feb 03 00:00:00 GMT 2010 Mon Aug 23 01:00:00 BST 2010
HCP-3_CENP-A_N2_EEMB_(OD00079_HCP3_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2541 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Fri Dec 04 00:00:00 GMT 2009 Thu Sep 09 01:00:00 BST 2010
HCP-3_CENP-A_N2_MXEMB_(OD00079_HCP3_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2446 binding_site_identification_design Fri Oct 09 01:00:00 BST 2009 Thu Oct 15 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
HTZ-1_N2_Mixed_Embryos (BK00001_HTZ1_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 43 binding_site_identification_design Thu Jan 17 00:00:00 GMT 2008 Mon Jul 28 01:00:00 BST 2008 Mon Aug 16 01:00:00 BST 2010
Histone_H3K20me1_N2_EEMB_(AB9051_H4K20ME1104513_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2765 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Mon Feb 01 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H3K27ac_N2_EEMB_(AB4729_H3K27AC361571_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2740 binding_site_identification_design Fri Jan 29 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
Histone_H3K27ac_N2_EEMB_(WA30634849_H3K27AC_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2727 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
Histone_H3K27ac_N2_L3_(AB4729_H3K27AC361571_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2771 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H3K27ac_N2_L3_(WA30634849_H3K27AC_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2773 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H3K27me3_N2_L3_(HK00013_H3K27ME31E7_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2400 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Thu Oct 08 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K36me1_N2_EEMB_(AB9048_H3K36ME1206009_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2604 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Thu Oct 21 01:00:00 BST 2010
Histone_H3K36me2_N2_EEMB_(AB9049_H3K36ME2608457_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2338 binding_site_identification_design Fri Aug 28 01:00:00 BST 2009 Wed Sep 30 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K36me2_N2_EEMB_(HK00012_H3K36ME22C3_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 909 binding_site_identification_design Fri Aug 28 01:00:00 BST 2009 Tue Sep 22 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K36me2_N2_L3_(HK00012_H3K36ME22C3_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2399 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Wed Oct 07 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K36me3_N2_EEMB_(HK00001_H3K36ME313C9_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 973 binding_site_identification_design Thu Aug 27 01:00:00 BST 2009 Tue Sep 22 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K36me3_N2_L3_(HK00001_H3K36ME313C9_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2401 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Thu Oct 08 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K36me3_from_N2_L3_larvae_(AB9050_H3K36ME3_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 175 binding_site_identification_design Wed Apr 16 01:00:00 BST 2008 Mon Feb 15 00:00:00 GMT 2010 Mon Aug 23 01:00:00 BST 2010
Histone_H3K4me1_N2_EEMB_(AB8895_H3K4ME1733246_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2726 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
Histone_H3K4me1_N2_L3_(AB8895_H3K4ME1733246_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2775 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H3K4me2_N2_EEMB_(MP07030_H3K4ME2DAM1570816_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2645 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Histone_H3K4me2_N2_EEMB_(WA30834809_H3K4ME2_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2445 binding_site_identification_design Thu Aug 27 01:00:00 BST 2009 Wed Oct 14 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K4me2_N2_L3_(WA30834809_H3K4ME2_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2402 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Thu Oct 08 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K4me3_N2_EEMB_(WA30534819_H3K4ME3_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2311 binding_site_identification_design Thu Aug 27 01:00:00 BST 2009 Tue Sep 22 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K4me3_from_N2_L3_larvae_(AR0169_H3K4me3_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 176 binding_site_identification_design Wed Apr 16 01:00:00 BST 2008 Mon Feb 15 00:00:00 GMT 2010 Mon Aug 23 01:00:00 BST 2010
Histone_H3K79me1_N2_EEMB_(AB2886_H3K79ME1361912_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2410 binding_site_identification_design Sun Oct 11 01:00:00 BST 2009 Mon Oct 12 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K79me2_N2_EEMB_(AB3594_H3K79ME2346021_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2442 binding_site_identification_design Sun Oct 11 01:00:00 BST 2009 Tue Oct 13 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K79me3_N2_EEMB_(AB2621_H3K79ME3361576_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2443 binding_site_identification_design Sun Oct 11 01:00:00 BST 2009 Tue Oct 13 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9ac_N2_EEMB_(AB4441_H3K9AC_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2728 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
Histone_H3K9me1_N2_EEMB_(AB8896_H3K9ME1104560_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2739 binding_site_identification_design Fri Jan 29 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
Histone_H3K9me1_N2_EEMB_(AB9045_H3K9ME1291918_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2646 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Histone_H3K9me1_N2_L3_(AB9045_H3K9ME1291918_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2770 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H3K9me2_N2_EEMB_(HK00008_H3K9ME26D11_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2444 binding_site_identification_design Fri Aug 28 01:00:00 BST 2009 Wed Oct 14 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9me2_N2_L3_(HK00008_H3K9ME26D11_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2406 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Sun Oct 11 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9me3_N2_EEMB_1_(UP07442_H3K9ME3_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 691 binding_site_identification_design Wed Feb 03 00:00:00 GMT 2010 Wed Feb 03 00:00:00 GMT 2010 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9me3_N2_EEMB_2_(AB8898_H3K9ME3339901_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2339 binding_site_identification_design Fri Aug 28 01:00:00 BST 2009 Wed Sep 30 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9me3_N2_EEMB_2_(HK00009_H3K9ME32F3_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 982 binding_site_identification_design Fri Aug 28 01:00:00 BST 2009 Tue Sep 22 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9me3_N2_L3_(HK00009_H3K9ME32F3_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2398 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Wed Oct 07 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3K9me3_from_N2_L3_larvae_(UP07442_H3K9ME3_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 188 binding_site_identification_design Wed Apr 09 01:00:00 BST 2008 Mon Feb 15 00:00:00 GMT 2010 Mon Aug 23 01:00:00 BST 2010
Histone_H3_N2_EEMB_1_(AR0144_H3144_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2312 binding_site_identification_design Thu May 14 01:00:00 BST 2009 Wed Sep 23 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3_N2_EEMB_2_(AB1791_H3609253_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2313 binding_site_identification_design Fri Aug 21 01:00:00 BST 2009 Wed Sep 23 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3_N2_L3_(AB1791_H3_N2_L3_new) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2409 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Mon Oct 12 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3_N2_L3_1_(AR0144_H3144_N2_L3_LM) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2408 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Sun Oct 11 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H3_N2_L3_2_(AR0144_H3144_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2407 binding_site_identification_design Wed Oct 07 01:00:00 BST 2009 Sun Oct 11 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Histone_H4K20me1_N2_L3_(AB9051_H4K20ME1104513_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2784 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H4K8ac_N2_L3_(AB15823_H4K8AC487128_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2774 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H4_N2_L3_(MP05858_H4DAM1636076_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2768 binding_site_identification_design Thu Feb 04 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Histone_H4_Tetra_ac_N2_EEMB_(LPAR109_H4TETRAAC109_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2766 binding_site_identification_design Wed Jan 13 00:00:00 GMT 2010 Mon Feb 01 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Intron Confirmation Chromosome I 2010-01-28 This experiment identifies intron boundary coordinates in C. elegans
genomic sequence. Initially, we run genefinder to predict protein-coding
transcripts from the C. elegans chromosome sequences. We align
existing cDNA and EST sequences to the predicted transcript sequences
to confirm the transcript structure. Predicted splice junctions
unconfirmed by these alignments are tested for confirmation using
RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
use gene-specific/gene-specific, 5' RACE/gene-specific, and
gene-specific/3' RACE primer pairs. 2718 transcript_identification_design Mon Feb 08 00:00:00 GMT 2010 Mon Feb 08 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
Intron Confirmation Chromosome II 2009-09-21 This experiment identifies intron boundary coordinates in C. elegans
genomic sequence. Initially, we run genefinder to predict protein-coding
transcripts from the C. elegans chromosome sequences. We align
existing cDNA and EST sequences to the predicted transcript sequences
to confirm the transcript structure. Predicted splice junctions
unconfirmed by these alignments are tested for confirmation using
RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
use gene-specific/gene-specific, 5' RACE/gene-specific, and
gene-specific/3' RACE primer pairs. 2304 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
L1 20dC 0hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 484 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L1 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2860 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Wed Nov 17 00:00:00 GMT 2010
L1 lin-35(n745) integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, and wormbase splice junctions Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2858 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
L2 25dC 14hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 472 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 A-class neuron tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 469 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 GABA neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 466 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2861 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Wed Nov 17 00:00:00 GMT 2010
L2 body wall muscle tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 465 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 coelomocytes tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 657 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
L2 excretory cell tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 464 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 glutamate receptor expressing neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 658 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
L2 intestine tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 463 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 panneural tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 462 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 polyA enriched 20dC 14hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 477 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L2 reference (mockIP) tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 461 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L3 25dC 25hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 474 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L3 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2862 transcript_identification_design Wed Feb 17 00:00:00 GMT 2010 Wed Feb 17 00:00:00 GMT 2010 Wed Nov 17 00:00:00 GMT 2010
L3-L4 PVD & OLL neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 460 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L3-L4 dopaminergic neuron tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 655 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
L3-L4 hypodermal cells tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 2454 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
L3-L4 reference (mockIP) tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 659 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
L4 25dC 36hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 473 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
L4 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2863 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Wed Nov 17 00:00:00 GMT 2010
L4 male integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2859 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Wed Nov 17 00:00:00 GMT 2010
LEM-2_N2_MXEMB_(SDQ3891_LEM2_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2729 binding_site_identification_design Thu Jan 28 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
MES-4_FLAG_Early_Embryos_(SGF3165_FLAG_MES4FLAG_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 195 binding_site_identification_design Wed Jul 09 01:00:00 BST 2008 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 23 01:00:00 BST 2010
MES-4_N2_EEMB_(SDQ0791_MES4_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 911 binding_site_identification_design Thu Mar 05 00:00:00 GMT 2009 Tue Sep 22 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
MIX-1_N2_Mixed_Embryos_(JL00004_MIX1_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 336 binding_site_identification_design Mon Aug 25 01:00:00 BST 2008 Mon Nov 30 00:00:00 GMT 2009 Mon Aug 23 01:00:00 BST 2010
MRG-1_N2_EEMB_(SDQ0790_MRG1_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 897 binding_site_identification_design Thu Mar 05 00:00:00 GMT 2009 Tue Sep 22 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Mononucleosomes_JK1107_AD We digested chromatin with Micrococcal Nuclease and isolated mononucleosome length fragment. These fragments were sequenced by Illumina Genome Analyzer II. Data was processed to obtain nucleosome density over entire genome. 2764 binding_site_identification_design Mon Feb 01 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
Mononucleosomes_N2_MXEMB We digested chromatin with Micrococcal Nuclease and isolated mononucleosome length fragment. These fragments were sequenced by Illumina Genome Analyzer II. Data was processed to obtain nucleosome density over entire genome. 2763 binding_site_identification_design Mon Feb 01 00:00:00 GMT 2010 Mon Feb 15 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
NA_MES4FLAG_EEMB The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 202 binding_site_identification_design Wed Jun 04 01:00:00 BST 2008 Thu Jan 15 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
NPP-13_N2_MXEMB_(SDQ3897_NPP13_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2738 binding_site_identification_design Thu Jan 28 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Fri Oct 29 01:00:00 BST 2010
Negative_control_Antibody_N2_EEMB_(SS00050_IGG_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2382 binding_site_identification_design Fri May 09 01:00:00 BST 2014 Tue Feb 10 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Negative_control_No_Antibody_N2_EEMB_(NA_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 2364 binding_site_identification_design Fri Aug 28 01:00:00 BST 2009 Wed Sep 30 01:00:00 BST 2009 Mon Aug 16 01:00:00 BST 2010
Negative_control_antibody_from_N2_L3_larvae_(JA00002_IGG_N2_L3) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 179 binding_site_identification_design Fri May 09 01:00:00 BST 2008 Mon Feb 15 00:00:00 GMT 2010 Mon Aug 16 01:00:00 BST 2010
POL-2_CTD_Mixed_Embryos (ABAB5408_4H8_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 174 binding_site_identification_design Sun Mar 16 00:00:00 GMT 2008 Mon Jun 02 01:00:00 BST 2008 Mon Aug 16 01:00:00 BST 2010
POL-2_N2_L4_(CVMMS126R_8WG16_N2_L4) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 599 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Mon Feb 23 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
POL-2_N2_Mixed_Embryos (CVMMS126R_8WG16_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 44 binding_site_identification_design Thu Jan 17 00:00:00 GMT 2008 Mon Jul 28 01:00:00 BST 2008 Mon Aug 16 01:00:00 BST 2010
Pol_II_CTD_N2_EEMB_(ABAB817_8WG16_N2_EEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 663 binding_site_identification_design Wed Feb 03 00:00:00 GMT 2010 Wed Feb 03 00:00:00 GMT 2010 Mon Aug 16 01:00:00 BST 2010
RNA-Seq - pathogen Harposporium 25dC 24hr exposure post-adulthood N2 genelets Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2587 transcript_identification_design Mon Feb 01 00:00:00 GMT 2010 Mon Feb 01 00:00:00 GMT 2010 Thu Oct 21 01:00:00 BST 2010
RNA-Seq - pathogen Harposporium 25dC 24hr exposure post-adulthood N2 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2580 transcript_identification_design Sun Jan 31 00:00:00 GMT 2010 Sun Jan 31 00:00:00 GMT 2010 Tue Oct 19 01:00:00 BST 2010
RNA-Seq - pathogen Harposporium control OP50 25dC 24hr exposure post-adulthood N2 genelets Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2588 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
RNA-Seq - pathogen Harposporium control OP50 25dC 24hr exposure post-adulthood N2 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2581 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Tue Oct 19 01:00:00 BST 2010
RNA-Seq - pathogen Smacescens control OP50 25dC 24hr exposure post-adulthood N2 genelets Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2591 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
RNA-Seq - pathogen Smacescens control OP50 25dC 24hr exposure post-adulthood N2 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2584 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Wed Oct 20 01:00:00 BST 2010
RNA-seq - pathogen Smacescens 25dC 24hr exposure post-adulthood N2 genelets Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2589 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
RNA-seq - pathogen Smacescens 25dC 24hr exposure post-adulthood N2 sequences & alignment Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2582 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Wed Oct 20 01:00:00 BST 2010
RNA-seq - soma-only mid-L4 25dC 36hrs post-L1 JK1107 genelets Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2590 transcript_identification_design Tue Feb 02 00:00:00 GMT 2010 Tue Feb 02 00:00:00 GMT 2010 Mon Nov 01 00:00:00 GMT 2010
RNA-seq - soma-only mid-L4 25dC 36hrs post-L1 JK1107 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2583 transcript_identification_design Wed Jan 20 00:00:00 GMT 2010 Wed Jan 20 00:00:00 GMT 2010 Wed Oct 20 01:00:00 BST 2010
RNAseq - 0911 aggregate fourteen stages - non-redundant exons Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2647 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Thu Nov 18 00:00:00 GMT 2010
RNAseq - Adult spe-9 23dC 8days post-L4 molt genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2835 transcript_identification_design Thu Feb 04 00:00:00 GMT 2010 Thu Feb 04 00:00:00 GMT 2010 Wed Nov 03 00:00:00 GMT 2010
RNAseq - Adult spe-9 23dC 8days post-L4 molt sequences & alignment Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2345 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Thu Aug 12 01:00:00 BST 2010
RNAseq - Young Adult 25dC 46hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2836 transcript_identification_design Thu Feb 04 00:00:00 GMT 2010 Thu Feb 04 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - Young Adult 25dC 46hrs post-L1 sequences & alignment Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2354 transcript_identification_design Sun Nov 15 00:00:00 GMT 2009 Sun Nov 15 00:00:00 GMT 2009 Sat Aug 14 01:00:00 BST 2010
RNAseq - dauer daf-2 25dC 91hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2837 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - dauer daf-2 25dC 91hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2347 transcript_identification_design Sat Nov 14 00:00:00 GMT 2009 Sat Nov 14 00:00:00 GMT 2009 Fri Aug 13 01:00:00 BST 2010
RNAseq - dauer entry daf-2 25dC 48hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2838 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - dauer entry daf-2 25dC 48hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2387 transcript_identification_design Sun Nov 15 00:00:00 GMT 2009 Sun Nov 15 00:00:00 GMT 2009 Sat Aug 14 01:00:00 BST 2010
RNAseq - dauer exit daf-2 25dC 91hrs 15dC 12hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2839 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - dauer exit daf-2 25dC 91hrs 15dC 12hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2349 transcript_identification_design Sat Nov 14 00:00:00 GMT 2009 Sat Nov 14 00:00:00 GMT 2009 Fri Aug 13 01:00:00 BST 2010
RNAseq - early embryo genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2840 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - early embryo sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2321 transcript_identification_design Thu Nov 12 00:00:00 GMT 2009 Thu Nov 12 00:00:00 GMT 2009 Wed Aug 11 01:00:00 BST 2010
RNAseq - embryo him-8 20dC post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2841 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - embryo him-8 20dC post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2346 transcript_identification_design Sat Nov 14 00:00:00 GMT 2009 Sat Nov 14 00:00:00 GMT 2009 Fri Aug 13 01:00:00 BST 2010
RNAseq - late embryo 20dC 4.5hrs post-early embryo genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2842 transcript_identification_design Wed Feb 10 00:00:00 GMT 2010 Wed Feb 10 00:00:00 GMT 2010 Tue Nov 09 00:00:00 GMT 2010
RNAseq - late embryo 20dC 4.5hrs post-early embryo sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2324 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Thu Aug 12 01:00:00 BST 2010
RNAseq - lin-35(n745) mid-L1 25dC 4.0hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2843 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - lin-35(n745) mid-L1 25dC 4.0hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2343 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Thu Aug 12 01:00:00 BST 2010
RNAseq - male mid-L4 dpy28(y1);him-8(e1489) 25dC 30hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2844 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - male mid-L4 dpy28(y1);him-8(e1489) 25dC 30hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2325 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Thu Aug 12 01:00:00 BST 2010
RNAseq - mid-L1 20dC 4hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2845 transcript_identification_design Thu Feb 11 00:00:00 GMT 2010 Thu Feb 11 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - mid-L1 20dC 4hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2326 transcript_identification_design Fri Nov 13 00:00:00 GMT 2009 Fri Nov 13 00:00:00 GMT 2009 Thu Aug 12 01:00:00 BST 2010
RNAseq - mid-L2 25dC 14 hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2351 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Fri Aug 13 01:00:00 BST 2010
RNAseq - mid-L2 25dC 14hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2846 transcript_identification_design Thu Feb 11 00:00:00 GMT 2010 Thu Feb 11 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - mid-L3 25dC 25hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2847 transcript_identification_design Fri Feb 05 00:00:00 GMT 2010 Fri Feb 05 00:00:00 GMT 2010 Thu Nov 04 00:00:00 GMT 2010
RNAseq - mid-L3 25dC 25hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2352 transcript_identification_design Fri Nov 20 00:00:00 GMT 2009 Fri Nov 20 00:00:00 GMT 2009 Fri Aug 13 01:00:00 BST 2010
RNAseq - mid-L4 25dC 36hrs post-L1 genelets revised Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2848 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 09 00:00:00 GMT 2010
RNAseq - mid-L4 25dC 36hrs post-L1 sequences & alignments Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2353 transcript_identification_design Fri Nov 20 00:00:00 GMT 2009 Fri Nov 20 00:00:00 GMT 2009 Sat Aug 14 01:00:00 BST 2010
SDC-2_N2_MXEMB_(SDQ3146_SDC2_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 645 binding_site_identification_design Mon Nov 30 00:00:00 GMT 2009 Mon Nov 30 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
SDC-2_N2_Mixed_Embryos (JL00005_SDC2_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 338 binding_site_identification_design Mon Nov 30 00:00:00 GMT 2009 Mon Feb 23 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
SDC-3_N2_MXEMB_1A_(JL00002_SDC3_N2_MXEMB_1_A) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 553 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
SDC-3_N2_MXEMB_1B_(JL00002_SDC3_N2_MXEMB_1_B) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 575 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
SDC-3_N2_Mixed_Embryos_(JL00002_SDC3_N2_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 127 binding_site_identification_design Fri Dec 01 00:00:00 GMT 2006 Sun Feb 11 00:00:00 GMT 2007 Mon Aug 16 01:00:00 BST 2010
SDC3_YPT47(V;X)_MXEMB(JL00002_SDC3_YPT47_MXEMB) The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. 701 binding_site_identification_design Thu Nov 26 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_ALR-1_GFP_L2 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2434 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_AMA1_GFP_L4YA We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 589 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_AMA1_POLII_L4YA We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 590 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_BLMP-1_GFP_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2612 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_CEH-14_GFP_L2 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 734 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_CEH-30_GFP_lemb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2620 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Sat Oct 30 01:00:00 BST 2010
Snyder_DAF16_GFP_L4YA We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 591 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_DAF16_POLII_L4YA We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 592 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_DPY27_GFP_emb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2416 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 20 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_EGL-27_GFP_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2621 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Sat Oct 30 01:00:00 BST 2010
Snyder_EGL5_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2453 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Sat Nov 28 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_EGL5_POLII_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2616 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Thu Feb 11 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_ELT-3_GFP_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2614 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_EOR-1_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2417 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_EOR-1_POLII_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2596 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 17 00:00:00 GMT 2010 Fri Oct 22 01:00:00 BST 2010
Snyder_GEI11_GFP_L4 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2451 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Sat Nov 28 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_HLH-1_GFP_emb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2431 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_HLH-8_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2418 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_HLH-8_POLII_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2597 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Thu Feb 11 00:00:00 GMT 2010 Fri Oct 22 01:00:00 BST 2010
Snyder_LIN-11_GFP_L2 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2429 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_LIN-13_GFP_emb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2613 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_LIN-15B_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2610 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Sat Oct 30 01:00:00 BST 2010
Snyder_LIN-39_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2432 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_MAB5_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 593 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_MAB5_POLII_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 594 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_MDL-1_GFP_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2601 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Fri Oct 22 01:00:00 BST 2010
Snyder_MEP-1_GFP_emb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2600 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_N2_POLII_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2437 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_N2_POLII_L2 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2438 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_N2_POLII_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2439 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_N2_POLII_L4 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2440 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_N2_POLII_YA We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2441 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Sat Nov 28 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_N2_POLII_eemb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2435 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Sat Nov 28 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_N2_POLII_lemb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2436 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_NHR-105_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2617 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Sat Oct 30 01:00:00 BST 2010
Snyder_NHR-6_GFP_L2 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2433 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PES1_GFP_L4 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2450 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Sat Nov 28 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_GFP_FedL1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 585 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_GFP_L2 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2452 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Thu Nov 26 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_GFP_YA We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2599 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Fri Oct 22 01:00:00 BST 2010
Snyder_PHA4_GFP_emb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 582 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_GFP_lemb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2598 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_PHA4_POLII_FedL1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 587 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_POLII_emb We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 586 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_StarvedL1_GFP We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 584 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PHA4_StarvedL1_POLII We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 588 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
Snyder_PQM-1_GFP_L3 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2623 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Sat Oct 30 01:00:00 BST 2010
Snyder_SKN-1_GFP_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2622 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Wed Feb 10 00:00:00 GMT 2010 Tue Oct 26 01:00:00 BST 2010
Snyder_UNC-130_GFP_L1 We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. 2430 binding_site_identification_design Mon Mar 02 00:00:00 GMT 2009 Fri Nov 27 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
UTRome_V2_3UTRs_multiple_evidences The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2745 transcript_identification_design Tue Feb 16 00:00:00 GMT 2010 Tue Feb 16 00:00:00 GMT 2010 Mon Nov 15 00:00:00 GMT 2010
Young Adult Cephalic sheath (CEPsh) tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 660 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
Young Adult N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2851 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
Young Adult reference (mockIP) tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 656 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
dauer daf-2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2852 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
dauer entry daf-2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2853 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
dauer exit daf-2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2854 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
early embryo 20dC 0-4hrs post-fertilization N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 476 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
early embryo N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2855 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
embryo A-class motor neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 654 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
embryo AVA neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 459 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
embryo GABA motor neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 468 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo all cells reference tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 456 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo body wall muscle tiling array (v2) Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 470 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo coelomocytes tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 458 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo dopaminergic neurons tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 467 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo germline precursor cells tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 661 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Fri Jun 25 01:00:00 BST 2010
embryo him-8 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2856 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
embryo hypodermal cells tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 662 transcript_identification_design Thu Nov 19 00:00:00 GMT 2009 Thu Nov 19 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
embryo intestine tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 457 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo panneural tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 455 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
embryo_BAG_neurons_tiling_array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 2499 transcript_identification_design Wed Feb 10 00:00:00 GMT 2010 Wed Feb 10 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
embryo_PVC_neurons_tiling_array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 2500 transcript_identification_design Tue Feb 16 00:00:00 GMT 2010 Tue Feb 16 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
embryo_pharyngeal_muscle_tiling_array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 2548 transcript_identification_design Wed Feb 10 00:00:00 GMT 2010 Wed Feb 10 00:00:00 GMT 2010 Mon Nov 08 00:00:00 GMT 2010
gonad from young adult 20dC 42hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 481 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
late embryo 20dC 6-12hrs post-fertilization N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 479 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
late embryo N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition. 2857 transcript_identification_design Thu Feb 18 00:00:00 GMT 2010 Thu Feb 18 00:00:00 GMT 2010 Tue Nov 16 00:00:00 GMT 2010
male L4 25dC 36hrs post-L1 CB4689 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 478 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
mid-L1_miRNA_expression_change We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. 2777 transcript_identification_design Sat Feb 13 00:00:00 GMT 2010 Sat Feb 13 00:00:00 GMT 2010 Sat Nov 13 00:00:00 GMT 2010
pathogen Efaecalis 25dC 24hr exposure post-adulthood N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. 487 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
pathogen Pluminscens 25dC 24hr exposure post-adulthood N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. 486 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
pathogen Smarcescens 25dC 24hr exposure post-adulthood N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. 489 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
pathogen Smarcescens 25dC 48hr exposure post-adulthood N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. 488 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
pathogen control OP50 25dC 24hr exposure post-adulthood N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. 491 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
pathogen control OP50 25dC 48hr exposure post-adulthood N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. 490 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
small RNA 454 sequencing of N2 young adults The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2831 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA 454 sequencing of eri-1(mg366) The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2827 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA 454 sequencing of fer-1 unfertilized oocytes The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2832 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA 454 sequencing of glp-4(bn2) The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2828 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA 454 sequencing of him-8 sperm The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2829 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA Illumina sequencing of N2 embryos The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2826 transcript_identification_design Sun Feb 14 00:00:00 GMT 2010 Sun Feb 14 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA Illumina sequencing of N2 whole worms The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2825 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA Illumina sequencing of eri-1(mg366) The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2821 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Thu Nov 11 00:00:00 GMT 2010
small RNA Illumina sequencing of fer-1 unfertilized oocytes The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2830 transcript_identification_design Sun Feb 14 00:00:00 GMT 2010 Sun Feb 14 00:00:00 GMT 2010 Tue Nov 02 00:00:00 GMT 2010
small RNA Illumina sequencing of glp-4(bn2) The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2822 transcript_identification_design Fri Feb 12 00:00:00 GMT 2010 Fri Feb 12 00:00:00 GMT 2010 Thu Nov 11 00:00:00 GMT 2010
small RNA Illumina sequencing of him-8 sperm The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues. 2823 transcript_identification_design Sun Feb 14 00:00:00 GMT 2010 Sun Feb 14 00:00:00 GMT 2010 Thu Nov 11 00:00:00 GMT 2010
small RNA N2 embryos RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 634 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
small RNA N2 mid-L1 RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 635 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
small RNA N2 mid-L2 RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 636 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
small RNA N2 mid-L3 RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 637 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
small RNA N2 mid-L4 RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 638 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
small RNA N2 young adult RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 639 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
small RNA him-8 males RNA-seq We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates. 640 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Mon Mar 15 00:00:00 GMT 2010
small RNAs from living one cell C. elegans embryos (GSM427345) We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis. 2489 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Tue Aug 17 01:00:00 BST 2010
smallRNA from mixed-stage embryos (GSM427346) We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis. 2483 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Mon Aug 16 01:00:00 BST 2010
smallRNAs from older, fixed C. elegans embryos (GSM427344) We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis. 2488 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Tue Aug 17 01:00:00 BST 2010
smallRNAs from one cell stage C. elegans embryos (GSM427301) We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis. 2486 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Tue Aug 17 01:00:00 BST 2010
smallRNAs from post-gastrulation C. elegans embryos (GSM427332) We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis. 2487 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Tue Aug 17 01:00:00 BST 2010
smallRNAs from two-to-four cell C. elegans embryos (GSM427297) We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis. 2485 transcript_identification_design Wed Nov 18 00:00:00 GMT 2009 Wed Nov 18 00:00:00 GMT 2009 Tue Aug 17 01:00:00 BST 2010
soma-only mid-L4 25dC 36hrs post-L1 JK1107 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 485 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010
young adult 25dC 42hrs post-L1 N2 tiling array Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. 475 transcript_identification_design Tue Nov 17 00:00:00 GMT 2009 Tue Nov 17 00:00:00 GMT 2009 Wed Jun 23 01:00:00 BST 2010


We Need to generate an ?Analysis object for each experiment:

See ModENCODE Analysis & metadata discussion