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| | | publicReleaseDate | | | publicReleaseDate |
| | | embargoDate | | | embargoDate |
| | + | | |
| | |- | | |- |
| | | 3' UTR 454 sequencing pilot - alignments | | | 3' UTR 454 sequencing pilot - alignments |
| Line 27: |
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| | | Thu Nov 12 00:00:00 GMT 2009 | | | Thu Nov 12 00:00:00 GMT 2009 |
| | | Tue Aug 03 01:00:00 BST 2010 | | | Tue Aug 03 01:00:00 BST 2010 |
| − | |
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| − | |-
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| − | | 3' UTR Staged 454 Sequencing - L1 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
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| − | | 2327
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| − | | transcript_identification_design
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| − | | Thu Nov 12 00:00:00 GMT 2009
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| − | | Thu Nov 12 00:00:00 GMT 2009
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| − | | Fri Aug 06 01:00:00 BST 2010
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| − | |
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| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L1 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2455
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| − | | transcript_identification_design
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| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Mon Jul 19 01:00:00 BST 2010
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| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L2 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2328
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| − | | transcript_identification_design
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| − | | Thu Nov 12 00:00:00 GMT 2009
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| − | | Thu Nov 12 00:00:00 GMT 2009
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| − | | Fri Aug 06 01:00:00 BST 2010
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| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L2 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2456
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L3 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2329
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| − | | transcript_identification_design
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| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Tue Aug 03 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L3 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2457
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 03 00:00:00 GMT 2009
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| − | | Tue Nov 03 00:00:00 GMT 2009
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| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L4 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2330
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 12 00:00:00 GMT 2009
| |
| − | | Thu Nov 12 00:00:00 GMT 2009
| |
| − | | Fri Aug 06 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - L4 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2458
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-11 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2334
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Tue Aug 03 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-11 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2464
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-2 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2335
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| − | | transcript_identification_design
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| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Tue Aug 03 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-2 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2461
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
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| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-7 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2336
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Fri Nov 13 00:00:00 GMT 2009
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| − | | Tue Aug 03 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-7 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2462
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-9 - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2337
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Aug 06 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - daf-9 - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2463
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - embryo - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2331
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 12 00:00:00 GMT 2009
| |
| − | | Thu Nov 12 00:00:00 GMT 2009
| |
| − | | Fri Aug 06 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - embryo - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2465
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - hermaphrodite adult - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2332
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Tue Aug 03 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - hermaphrodite adult - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2460
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - male adult - alignments
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2333
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Aug 06 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTR Staged 454 Sequencing - male adult - sequences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2459
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Wed Nov 04 00:00:00 GMT 2009
| |
| − | | Mon Jul 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTRome 454 sequencing & alignment
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2482
| |
| − | | transcript_identification_design
| |
| − | | Mon Nov 16 00:00:00 GMT 2009
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| − | | Mon Nov 16 00:00:00 GMT 2009
| |
| − | | Mon Aug 09 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTRome Solexa sequencing & alignment
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2484
| |
| − | | transcript_identification_design
| |
| − | | Mon Nov 16 00:00:00 GMT 2009
| |
| − | | Mon Nov 16 00:00:00 GMT 2009
| |
| − | | Mon Aug 09 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3' UTRome sample pools
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2501
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Mon Aug 09 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3'UTR 454 sequencing pilot - Level 1
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 896
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 03 00:00:00 GMT 2009
| |
| − | | Tue Nov 03 00:00:00 GMT 2009
| |
| − | | Fri Jun 04 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 3'UTRome CEUP1 sequences, alignments, annotation
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 515
| |
| − | | transcript_identification_design
| |
| − | | Mon Nov 16 00:00:00 GMT 2009
| |
| − | | Mon Nov 16 00:00:00 GMT 2009
| |
| − | | Wed Aug 04 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 600_mM_Embryonic_Salt_Extracted_Chromatin
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2532
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 600_mM_Embryonic_Salt_Extracted_Chromatin_Pellet
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2533
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | 80_mM_Embryonic_Salt_Extracted_Chromatin
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2531
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Adult spe-9 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2850
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Adult_Mononucleosomes
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2538
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Ahringer_L3_Worm_Samples_1
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2539
| |
| − | | development_or_differentiation_design
| |
| − | | Fri Jul 10 01:00:00 BST 2009
| |
| − | | Mon Aug 10 01:00:00 BST 2009
| |
| − | | Sun Aug 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Ahringer_L3_Worm_Samples_2
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2540
| |
| − | | development_or_differentiation_design
| |
| − | | Fri Jul 10 01:00:00 BST 2009
| |
| − | | Mon Aug 10 01:00:00 BST 2009
| |
| − | | Mon Aug 30 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | C. elegans Intron Identification set.20090106.2_3_2_2_4_3_3_2_2
| |
| − | | This experiment identifies intron boundary coordinates in C. elegans
| |
| − | |-
| |
| − | | genomic sequence. Initially, we run genefinder to predict protein-coding
| |
| − | |-
| |
| − | | transcripts from the C. elegans chromosome sequences. We align
| |
| − | |-
| |
| − | | existing cDNA and EST sequences to the predicted transcript sequences
| |
| − | |-
| |
| − | | to confirm the transcript structure. Predicted splice junctions
| |
| − | |-
| |
| − | | unconfirmed by these alignments are tested for confirmation using
| |
| − | |-
| |
| − | | RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
| |
| − | |-
| |
| − | | use gene-specific/gene-specific, 5' RACE/gene-specific, and
| |
| − | |-
| |
| − | | gene-specific/3' RACE primer pairs.
| |
| − | | 448
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Sun Oct 25 01:00:00 BST 2009
| |
| − | |
| |
| − | |-
| |
| − | | C. elegans Intron Identification set.20090106.2_3_2_2_4_4_3_2_2
| |
| − | | This experiment identifies intron boundary coordinates in C. elegans
| |
| − | |-
| |
| − | | genomic sequence. Initially, we run genefinder to predict protein-coding
| |
| − | |-
| |
| − | | transcripts from the C. elegans chromosome sequences. We align
| |
| − | |-
| |
| − | | existing cDNA and EST sequences to the predicted transcript sequences
| |
| − | |-
| |
| − | | to confirm the transcript structure. Predicted splice junctions
| |
| − | |-
| |
| − | | unconfirmed by these alignments are tested for confirmation using
| |
| − | |-
| |
| − | | RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
| |
| − | |-
| |
| − | | use gene-specific/gene-specific, 5' RACE/gene-specific, and
| |
| − | |-
| |
| − | | gene-specific/3' RACE primer pairs.
| |
| − | | 446
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Sun Oct 25 01:00:00 BST 2009
| |
| − | |
| |
| − | |-
| |
| − | | C. elegans Intron Identification set.20090106.2_3_2_2_5_3_3_2_2
| |
| − | | This experiment identifies intron boundary coordinates in C. elegans
| |
| − | |-
| |
| − | | genomic sequence. Initially, we run genefinder to predict protein-coding
| |
| − | |-
| |
| − | | transcripts from the C. elegans chromosome sequences. We align
| |
| − | |-
| |
| − | | existing cDNA and EST sequences to the predicted transcript sequences
| |
| − | |-
| |
| − | | to confirm the transcript structure. Predicted splice junctions
| |
| − | |-
| |
| − | | unconfirmed by these alignments are tested for confirmation using
| |
| − | |-
| |
| − | | RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
| |
| − | |-
| |
| − | | use gene-specific/gene-specific, 5' RACE/gene-specific, and
| |
| − | |-
| |
| − | | gene-specific/3' RACE primer pairs.
| |
| − | | 447
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Sat Oct 31 00:00:00 GMT 2009
| |
| − | |
| |
| − | |-
| |
| − | | C. elegans Intron Identification.set.20090106.2_3_2_2_3_2_2_2_2
| |
| − | | This experiment identifies intron boundary coordinates in C. elegans
| |
| − | |-
| |
| − | | genomic sequence. Initially, we run genefinder to predict protein-coding
| |
| − | |-
| |
| − | | transcripts from the C. elegans chromosome sequences. We align
| |
| − | |-
| |
| − | | existing cDNA and EST sequences to the predicted transcript sequences
| |
| − | |-
| |
| − | | to confirm the transcript structure. Predicted splice junctions
| |
| − | |-
| |
| − | | unconfirmed by these alignments are tested for confirmation using
| |
| − | |-
| |
| − | | RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
| |
| − | |-
| |
| − | | use gene-specific/gene-specific, 5' RACE/gene-specific, and
| |
| − | |-
| |
| − | | gene-specific/3' RACE primer pairs.
| |
| − | | 445
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Sun Oct 25 01:00:00 BST 2009
| |
| − | |
| |
| − | |-
| |
| − | | CBP-1_N2_MXEMB_(SDQ3582_CBP1_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2767
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY-26_N2_Mixed_Embryos_(JL00003_DPY26_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 334
| |
| − | | binding_site_identification_design
| |
| − | | Mon Aug 25 01:00:00 BST 2008
| |
| − | | Mon Nov 30 00:00:00 GMT 2009
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY-27_N2_L4(JL00001_DPY27_N2_L4)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 630
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY-27_N2_MXEMB_1A_(JL00001_DPY27_N2_MXEMB_1_A)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 578
| |
| − | | binding_site_identification_design
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY-27_N2_Mixed_Embryos (JL00001_DPY27_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 90
| |
| − | | binding_site_identification_design
| |
| − | | Fri Dec 01 00:00:00 GMT 2006
| |
| − | | Sun Feb 11 00:00:00 GMT 2007
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY-28_N2_MXEMB_(JL00012_DPY28_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 644
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY27_11DH(I;X)_MXEMB(JL00001_DPY27_11DH_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 693
| |
| − | | binding_site_identification_design
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY27_YPT41(II;X)_MXEMB(JL00001_DPY27_YPT41_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 695
| |
| − | | binding_site_identification_design
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | DPY27_YPT47(V;X)_MXEMB(JL00001_DPY27_YPT47_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 696
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Day0_spe-9 Solexa Raw Data SOAP-aligned
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2730
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Day12_spe-9 Solexa Raw Data SOAP-aligned
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2734
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Day12_spe-9_23dC_miRNA_expression_change
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2788
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Nov 13 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Day5_spe-9 Solexa Raw Data SOAP-aligned
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2732
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Day8_spe-9 Solexa Raw Data SOAP-aligned
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2733
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Embryo_miRNA_expression_change
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2776
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Nov 13 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Embryonic_Mononucleosomes
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2537
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | H3.3_350_mM_Salt_Extracted_Chromatin
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2535
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | H3.3_600_mM_Salt_Extracted_Chromatin
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2536
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | H3.3_80_mM_Salt_Extracted_Chromatin
| |
| − | | We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical ??active?? chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and generate low-salt soluble nucleosomes. Nearly quantitative recovery of chromatin is obtained with 600 mM NaCl; however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin are rich in sequences that correspond to epigenetic regulatory elements genome-wide. The presence of active chromatin at both extremes of salt solubility suggests that these salt fractions capture bound and
| |
| − | |-
| |
| − | | unbound intermediates in active processes, thus providing a simple, powerful strategy for mapping epigenome dynamics.
| |
| − | | 2534
| |
| − | | binding_site_identification_design
| |
| − | | Mon Dec 15 00:00:00 GMT 2008
| |
| − | | Sun Nov 29 00:00:00 GMT 2009
| |
| − | | Fri Aug 27 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | HCP-3_CENP-A_Mixed_Embryos_(OD00001_HCP3_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 194
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jul 02 01:00:00 BST 2008
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | HCP-3_CENP-A_N2_EEMB_(OD00079_HCP3_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2541
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Fri Dec 04 00:00:00 GMT 2009
| |
| − | | Thu Sep 09 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | HCP-3_CENP-A_N2_MXEMB_(OD00079_HCP3_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2446
| |
| − | | binding_site_identification_design
| |
| − | | Fri Oct 09 01:00:00 BST 2009
| |
| − | | Thu Oct 15 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | HTZ-1_N2_Mixed_Embryos (BK00001_HTZ1_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 43
| |
| − | | binding_site_identification_design
| |
| − | | Thu Jan 17 00:00:00 GMT 2008
| |
| − | | Mon Jul 28 01:00:00 BST 2008
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K20me1_N2_EEMB_(AB9051_H4K20ME1104513_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2765
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K27ac_N2_EEMB_(AB4729_H3K27AC361571_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2740
| |
| − | | binding_site_identification_design
| |
| − | | Fri Jan 29 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K27ac_N2_EEMB_(WA30634849_H3K27AC_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2727
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K27ac_N2_L3_(AB4729_H3K27AC361571_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2771
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K27ac_N2_L3_(WA30634849_H3K27AC_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2773
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K27me3_N2_L3_(HK00013_H3K27ME31E7_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2400
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Thu Oct 08 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me1_N2_EEMB_(AB9048_H3K36ME1206009_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2604
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Thu Oct 21 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me2_N2_EEMB_(AB9049_H3K36ME2608457_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2338
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 28 01:00:00 BST 2009
| |
| − | | Wed Sep 30 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me2_N2_EEMB_(HK00012_H3K36ME22C3_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 909
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 28 01:00:00 BST 2009
| |
| − | | Tue Sep 22 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me2_N2_L3_(HK00012_H3K36ME22C3_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2399
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me3_N2_EEMB_(HK00001_H3K36ME313C9_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 973
| |
| − | | binding_site_identification_design
| |
| − | | Thu Aug 27 01:00:00 BST 2009
| |
| − | | Tue Sep 22 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me3_N2_L3_(HK00001_H3K36ME313C9_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2401
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Thu Oct 08 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K36me3_from_N2_L3_larvae_(AB9050_H3K36ME3_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 175
| |
| − | | binding_site_identification_design
| |
| − | | Wed Apr 16 01:00:00 BST 2008
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me1_N2_EEMB_(AB8895_H3K4ME1733246_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2726
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me1_N2_L3_(AB8895_H3K4ME1733246_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2775
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me2_N2_EEMB_(MP07030_H3K4ME2DAM1570816_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2645
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me2_N2_EEMB_(WA30834809_H3K4ME2_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2445
| |
| − | | binding_site_identification_design
| |
| − | | Thu Aug 27 01:00:00 BST 2009
| |
| − | | Wed Oct 14 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me2_N2_L3_(WA30834809_H3K4ME2_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2402
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Thu Oct 08 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me3_N2_EEMB_(WA30534819_H3K4ME3_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2311
| |
| − | | binding_site_identification_design
| |
| − | | Thu Aug 27 01:00:00 BST 2009
| |
| − | | Tue Sep 22 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K4me3_from_N2_L3_larvae_(AR0169_H3K4me3_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 176
| |
| − | | binding_site_identification_design
| |
| − | | Wed Apr 16 01:00:00 BST 2008
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K79me1_N2_EEMB_(AB2886_H3K79ME1361912_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2410
| |
| − | | binding_site_identification_design
| |
| − | | Sun Oct 11 01:00:00 BST 2009
| |
| − | | Mon Oct 12 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K79me2_N2_EEMB_(AB3594_H3K79ME2346021_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2442
| |
| − | | binding_site_identification_design
| |
| − | | Sun Oct 11 01:00:00 BST 2009
| |
| − | | Tue Oct 13 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K79me3_N2_EEMB_(AB2621_H3K79ME3361576_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2443
| |
| − | | binding_site_identification_design
| |
| − | | Sun Oct 11 01:00:00 BST 2009
| |
| − | | Tue Oct 13 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9ac_N2_EEMB_(AB4441_H3K9AC_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2728
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me1_N2_EEMB_(AB8896_H3K9ME1104560_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2739
| |
| − | | binding_site_identification_design
| |
| − | | Fri Jan 29 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me1_N2_EEMB_(AB9045_H3K9ME1291918_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2646
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me1_N2_L3_(AB9045_H3K9ME1291918_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2770
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me2_N2_EEMB_(HK00008_H3K9ME26D11_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2444
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 28 01:00:00 BST 2009
| |
| − | | Wed Oct 14 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me2_N2_L3_(HK00008_H3K9ME26D11_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2406
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Sun Oct 11 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me3_N2_EEMB_1_(UP07442_H3K9ME3_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 691
| |
| − | | binding_site_identification_design
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me3_N2_EEMB_2_(AB8898_H3K9ME3339901_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2339
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 28 01:00:00 BST 2009
| |
| − | | Wed Sep 30 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me3_N2_EEMB_2_(HK00009_H3K9ME32F3_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 982
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 28 01:00:00 BST 2009
| |
| − | | Tue Sep 22 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me3_N2_L3_(HK00009_H3K9ME32F3_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2398
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3K9me3_from_N2_L3_larvae_(UP07442_H3K9ME3_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 188
| |
| − | | binding_site_identification_design
| |
| − | | Wed Apr 09 01:00:00 BST 2008
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3_N2_EEMB_1_(AR0144_H3144_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2312
| |
| − | | binding_site_identification_design
| |
| − | | Thu May 14 01:00:00 BST 2009
| |
| − | | Wed Sep 23 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3_N2_EEMB_2_(AB1791_H3609253_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2313
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 21 01:00:00 BST 2009
| |
| − | | Wed Sep 23 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3_N2_L3_(AB1791_H3_N2_L3_new)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2409
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Mon Oct 12 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3_N2_L3_1_(AR0144_H3144_N2_L3_LM)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2408
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Sun Oct 11 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H3_N2_L3_2_(AR0144_H3144_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2407
| |
| − | | binding_site_identification_design
| |
| − | | Wed Oct 07 01:00:00 BST 2009
| |
| − | | Sun Oct 11 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H4K20me1_N2_L3_(AB9051_H4K20ME1104513_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2784
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H4K8ac_N2_L3_(AB15823_H4K8AC487128_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2774
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H4_N2_L3_(MP05858_H4DAM1636076_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2768
| |
| − | | binding_site_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Histone_H4_Tetra_ac_N2_EEMB_(LPAR109_H4TETRAAC109_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2766
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jan 13 00:00:00 GMT 2010
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Intron Confirmation Chromosome I 2010-01-28
| |
| − | | This experiment identifies intron boundary coordinates in C. elegans
| |
| − | |-
| |
| − | | genomic sequence. Initially, we run genefinder to predict protein-coding
| |
| − | |-
| |
| − | | transcripts from the C. elegans chromosome sequences. We align
| |
| − | |-
| |
| − | | existing cDNA and EST sequences to the predicted transcript sequences
| |
| − | |-
| |
| − | | to confirm the transcript structure. Predicted splice junctions
| |
| − | |-
| |
| − | | unconfirmed by these alignments are tested for confirmation using
| |
| − | |-
| |
| − | | RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
| |
| − | |-
| |
| − | | use gene-specific/gene-specific, 5' RACE/gene-specific, and
| |
| − | |-
| |
| − | | gene-specific/3' RACE primer pairs.
| |
| − | | 2718
| |
| − | | transcript_identification_design
| |
| − | | Mon Feb 08 00:00:00 GMT 2010
| |
| − | | Mon Feb 08 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Intron Confirmation Chromosome II 2009-09-21
| |
| − | | This experiment identifies intron boundary coordinates in C. elegans
| |
| − | |-
| |
| − | | genomic sequence. Initially, we run genefinder to predict protein-coding
| |
| − | |-
| |
| − | | transcripts from the C. elegans chromosome sequences. We align
| |
| − | |-
| |
| − | | existing cDNA and EST sequences to the predicted transcript sequences
| |
| − | |-
| |
| − | | to confirm the transcript structure. Predicted splice junctions
| |
| − | |-
| |
| − | | unconfirmed by these alignments are tested for confirmation using
| |
| − | |-
| |
| − | | RT-PCR, DNA sequencing, and sequence alignment. These PCR experiments
| |
| − | |-
| |
| − | | use gene-specific/gene-specific, 5' RACE/gene-specific, and
| |
| − | |-
| |
| − | | gene-specific/3' RACE primer pairs.
| |
| − | | 2304
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L1 20dC 0hrs post-L1 N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 484
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L1 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2860
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Wed Nov 17 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | L1 lin-35(n745) integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, and wormbase splice junctions
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2858
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 25dC 14hrs post-L1 N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 472
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 A-class neuron tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 469
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 GABA neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 466
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2861
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Wed Nov 17 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 body wall muscle tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 465
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 coelomocytes tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 657
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 excretory cell tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 464
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 glutamate receptor expressing neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 658
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 intestine tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 463
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 panneural tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 462
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 polyA enriched 20dC 14hrs post-L1 N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 477
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L2 reference (mockIP) tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 461
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L3 25dC 25hrs post-L1 N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 474
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L3 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2862
| |
| − | | transcript_identification_design
| |
| − | | Wed Feb 17 00:00:00 GMT 2010
| |
| − | | Wed Feb 17 00:00:00 GMT 2010
| |
| − | | Wed Nov 17 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | L3-L4 PVD & OLL neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 460
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L3-L4 dopaminergic neuron tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 655
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L3-L4 hypodermal cells tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 2454
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L3-L4 reference (mockIP) tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 659
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L4 25dC 36hrs post-L1 N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 473
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | L4 N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2863
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Wed Nov 17 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | L4 male integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2859
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Wed Nov 17 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | LEM-2_N2_MXEMB_(SDQ3891_LEM2_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2729
| |
| − | | binding_site_identification_design
| |
| − | | Thu Jan 28 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | MES-4_FLAG_Early_Embryos_(SGF3165_FLAG_MES4FLAG_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 195
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jul 09 01:00:00 BST 2008
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | MES-4_N2_EEMB_(SDQ0791_MES4_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 911
| |
| − | | binding_site_identification_design
| |
| − | | Thu Mar 05 00:00:00 GMT 2009
| |
| − | | Tue Sep 22 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | MIX-1_N2_Mixed_Embryos_(JL00004_MIX1_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 336
| |
| − | | binding_site_identification_design
| |
| − | | Mon Aug 25 01:00:00 BST 2008
| |
| − | | Mon Nov 30 00:00:00 GMT 2009
| |
| − | | Mon Aug 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | MRG-1_N2_EEMB_(SDQ0790_MRG1_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 897
| |
| − | | binding_site_identification_design
| |
| − | | Thu Mar 05 00:00:00 GMT 2009
| |
| − | | Tue Sep 22 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Mononucleosomes_JK1107_AD
| |
| − | | We digested chromatin with Micrococcal Nuclease and isolated mononucleosome length fragment. These fragments were sequenced by Illumina Genome Analyzer II. Data was processed to obtain nucleosome density over entire genome.
| |
| − | | 2764
| |
| − | | binding_site_identification_design
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Mononucleosomes_N2_MXEMB
| |
| − | | We digested chromatin with Micrococcal Nuclease and isolated mononucleosome length fragment. These fragments were sequenced by Illumina Genome Analyzer II. Data was processed to obtain nucleosome density over entire genome.
| |
| − | | 2763
| |
| − | | binding_site_identification_design
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | NA_MES4FLAG_EEMB
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 202
| |
| − | | binding_site_identification_design
| |
| − | | Wed Jun 04 01:00:00 BST 2008
| |
| − | | Thu Jan 15 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | NPP-13_N2_MXEMB_(SDQ3897_NPP13_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2738
| |
| − | | binding_site_identification_design
| |
| − | | Thu Jan 28 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Fri Oct 29 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Negative_control_Antibody_N2_EEMB_(SS00050_IGG_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2382
| |
| − | | binding_site_identification_design
| |
| − | | Fri May 09 01:00:00 BST 2014
| |
| − | | Tue Feb 10 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Negative_control_No_Antibody_N2_EEMB_(NA_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 2364
| |
| − | | binding_site_identification_design
| |
| − | | Fri Aug 28 01:00:00 BST 2009
| |
| − | | Wed Sep 30 01:00:00 BST 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Negative_control_antibody_from_N2_L3_larvae_(JA00002_IGG_N2_L3)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 179
| |
| − | | binding_site_identification_design
| |
| − | | Fri May 09 01:00:00 BST 2008
| |
| − | | Mon Feb 15 00:00:00 GMT 2010
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | POL-2_CTD_Mixed_Embryos (ABAB5408_4H8_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 174
| |
| − | | binding_site_identification_design
| |
| − | | Sun Mar 16 00:00:00 GMT 2008
| |
| − | | Mon Jun 02 01:00:00 BST 2008
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | POL-2_N2_L4_(CVMMS126R_8WG16_N2_L4)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 599
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Feb 23 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | POL-2_N2_Mixed_Embryos (CVMMS126R_8WG16_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 44
| |
| − | | binding_site_identification_design
| |
| − | | Thu Jan 17 00:00:00 GMT 2008
| |
| − | | Mon Jul 28 01:00:00 BST 2008
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Pol_II_CTD_N2_EEMB_(ABAB817_8WG16_N2_EEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 663
| |
| − | | binding_site_identification_design
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Wed Feb 03 00:00:00 GMT 2010
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-Seq - pathogen Harposporium 25dC 24hr exposure post-adulthood N2 genelets
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2587
| |
| − | | transcript_identification_design
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Mon Feb 01 00:00:00 GMT 2010
| |
| − | | Thu Oct 21 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-Seq - pathogen Harposporium 25dC 24hr exposure post-adulthood N2 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2580
| |
| − | | transcript_identification_design
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Sun Jan 31 00:00:00 GMT 2010
| |
| − | | Tue Oct 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-Seq - pathogen Harposporium control OP50 25dC 24hr exposure post-adulthood N2 genelets
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2588
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-Seq - pathogen Harposporium control OP50 25dC 24hr exposure post-adulthood N2 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2581
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Oct 19 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-Seq - pathogen Smacescens control OP50 25dC 24hr exposure post-adulthood N2 genelets
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2591
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-Seq - pathogen Smacescens control OP50 25dC 24hr exposure post-adulthood N2 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2584
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Wed Oct 20 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-seq - pathogen Smacescens 25dC 24hr exposure post-adulthood N2 genelets
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2589
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-seq - pathogen Smacescens 25dC 24hr exposure post-adulthood N2 sequences & alignment
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2582
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Wed Oct 20 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-seq - soma-only mid-L4 25dC 36hrs post-L1 JK1107 genelets
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2590
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Tue Feb 02 00:00:00 GMT 2010
| |
| − | | Mon Nov 01 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNA-seq - soma-only mid-L4 25dC 36hrs post-L1 JK1107 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2583
| |
| − | | transcript_identification_design
| |
| − | | Wed Jan 20 00:00:00 GMT 2010
| |
| − | | Wed Jan 20 00:00:00 GMT 2010
| |
| − | | Wed Oct 20 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - 0911 aggregate fourteen stages - non-redundant exons
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2647
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Nov 18 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - Adult spe-9 23dC 8days post-L4 molt genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2835
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Wed Nov 03 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - Adult spe-9 23dC 8days post-L4 molt sequences & alignment
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2345
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Thu Aug 12 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - Young Adult 25dC 46hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2836
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Thu Feb 04 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - Young Adult 25dC 46hrs post-L1 sequences & alignment
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2354
| |
| − | | transcript_identification_design
| |
| − | | Sun Nov 15 00:00:00 GMT 2009
| |
| − | | Sun Nov 15 00:00:00 GMT 2009
| |
| − | | Sat Aug 14 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - dauer daf-2 25dC 91hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2837
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - dauer daf-2 25dC 91hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2347
| |
| − | | transcript_identification_design
| |
| − | | Sat Nov 14 00:00:00 GMT 2009
| |
| − | | Sat Nov 14 00:00:00 GMT 2009
| |
| − | | Fri Aug 13 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - dauer entry daf-2 25dC 48hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2838
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - dauer entry daf-2 25dC 48hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2387
| |
| − | | transcript_identification_design
| |
| − | | Sun Nov 15 00:00:00 GMT 2009
| |
| − | | Sun Nov 15 00:00:00 GMT 2009
| |
| − | | Sat Aug 14 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - dauer exit daf-2 25dC 91hrs 15dC 12hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2839
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - dauer exit daf-2 25dC 91hrs 15dC 12hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2349
| |
| − | | transcript_identification_design
| |
| − | | Sat Nov 14 00:00:00 GMT 2009
| |
| − | | Sat Nov 14 00:00:00 GMT 2009
| |
| − | | Fri Aug 13 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - early embryo genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2840
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - early embryo sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2321
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 12 00:00:00 GMT 2009
| |
| − | | Thu Nov 12 00:00:00 GMT 2009
| |
| − | | Wed Aug 11 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - embryo him-8 20dC post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2841
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - embryo him-8 20dC post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2346
| |
| − | | transcript_identification_design
| |
| − | | Sat Nov 14 00:00:00 GMT 2009
| |
| − | | Sat Nov 14 00:00:00 GMT 2009
| |
| − | | Fri Aug 13 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - late embryo 20dC 4.5hrs post-early embryo genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2842
| |
| − | | transcript_identification_design
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Nov 09 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - late embryo 20dC 4.5hrs post-early embryo sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2324
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Thu Aug 12 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - lin-35(n745) mid-L1 25dC 4.0hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2843
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - lin-35(n745) mid-L1 25dC 4.0hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2343
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Thu Aug 12 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - male mid-L4 dpy28(y1);him-8(e1489) 25dC 30hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2844
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - male mid-L4 dpy28(y1);him-8(e1489) 25dC 30hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2325
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Thu Aug 12 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L1 20dC 4hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2845
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 11 00:00:00 GMT 2010
| |
| − | | Thu Feb 11 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L1 20dC 4hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2326
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Fri Nov 13 00:00:00 GMT 2009
| |
| − | | Thu Aug 12 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L2 25dC 14 hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2351
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Fri Aug 13 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L2 25dC 14hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2846
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 11 00:00:00 GMT 2010
| |
| − | | Thu Feb 11 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L3 25dC 25hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2847
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Fri Feb 05 00:00:00 GMT 2010
| |
| − | | Thu Nov 04 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L3 25dC 25hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2352
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 20 00:00:00 GMT 2009
| |
| − | | Fri Nov 20 00:00:00 GMT 2009
| |
| − | | Fri Aug 13 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L4 25dC 36hrs post-L1 genelets revised
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2848
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 09 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | RNAseq - mid-L4 25dC 36hrs post-L1 sequences & alignments
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2353
| |
| − | | transcript_identification_design
| |
| − | | Fri Nov 20 00:00:00 GMT 2009
| |
| − | | Fri Nov 20 00:00:00 GMT 2009
| |
| − | | Sat Aug 14 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | SDC-2_N2_MXEMB_(SDQ3146_SDC2_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 645
| |
| − | | binding_site_identification_design
| |
| − | | Mon Nov 30 00:00:00 GMT 2009
| |
| − | | Mon Nov 30 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | SDC-2_N2_Mixed_Embryos (JL00005_SDC2_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 338
| |
| − | | binding_site_identification_design
| |
| − | | Mon Nov 30 00:00:00 GMT 2009
| |
| − | | Mon Feb 23 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | SDC-3_N2_MXEMB_1A_(JL00002_SDC3_N2_MXEMB_1_A)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 553
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | SDC-3_N2_MXEMB_1B_(JL00002_SDC3_N2_MXEMB_1_B)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 575
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | SDC-3_N2_Mixed_Embryos_(JL00002_SDC3_N2_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 127
| |
| − | | binding_site_identification_design
| |
| − | | Fri Dec 01 00:00:00 GMT 2006
| |
| − | | Sun Feb 11 00:00:00 GMT 2007
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | SDC3_YPT47(V;X)_MXEMB(JL00002_SDC3_YPT47_MXEMB)
| |
| − | | The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
| |
| − | |-
| |
| − | | We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
| |
| − | | 701
| |
| − | | binding_site_identification_design
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_ALR-1_GFP_L2
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2434
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_AMA1_GFP_L4YA
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 589
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_AMA1_POLII_L4YA
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 590
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_BLMP-1_GFP_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2612
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_CEH-14_GFP_L2
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 734
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_CEH-30_GFP_lemb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2620
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Sat Oct 30 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_DAF16_GFP_L4YA
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 591
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_DAF16_POLII_L4YA
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 592
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_DPY27_GFP_emb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2416
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 20 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_EGL-27_GFP_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2621
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Sat Oct 30 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_EGL5_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2453
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Sat Nov 28 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_EGL5_POLII_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2616
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Thu Feb 11 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_ELT-3_GFP_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2614
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_EOR-1_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2417
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_EOR-1_POLII_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2596
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 17 00:00:00 GMT 2010
| |
| − | | Fri Oct 22 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_GEI11_GFP_L4
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2451
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Sat Nov 28 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_HLH-1_GFP_emb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2431
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_HLH-8_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2418
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_HLH-8_POLII_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2597
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Thu Feb 11 00:00:00 GMT 2010
| |
| − | | Fri Oct 22 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_LIN-11_GFP_L2
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2429
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_LIN-13_GFP_emb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2613
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_LIN-15B_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2610
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Sat Oct 30 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_LIN-39_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2432
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_MAB5_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 593
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_MAB5_POLII_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 594
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_MDL-1_GFP_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2601
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Fri Oct 22 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_MEP-1_GFP_emb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2600
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2437
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_L2
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2438
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2439
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_L4
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2440
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_YA
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2441
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Sat Nov 28 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_eemb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2435
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Sat Nov 28 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_N2_POLII_lemb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2436
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_NHR-105_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2617
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Sat Oct 30 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_NHR-6_GFP_L2
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2433
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PES1_GFP_L4
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2450
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Sat Nov 28 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_GFP_FedL1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 585
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_GFP_L2
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2452
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Thu Nov 26 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_GFP_YA
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2599
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Fri Oct 22 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_GFP_emb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 582
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_GFP_lemb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2598
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_POLII_FedL1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 587
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_POLII_emb
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 586
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_StarvedL1_GFP
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 584
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PHA4_StarvedL1_POLII
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 588
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_PQM-1_GFP_L3
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2623
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Sat Oct 30 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_SKN-1_GFP_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2622
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Tue Oct 26 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Snyder_UNC-130_GFP_L1
| |
| − | | We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.
| |
| − | | 2430
| |
| − | | binding_site_identification_design
| |
| − | | Mon Mar 02 00:00:00 GMT 2009
| |
| − | | Fri Nov 27 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | UTRome_V2_3UTRs_multiple_evidences
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2745
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 16 00:00:00 GMT 2010
| |
| − | | Tue Feb 16 00:00:00 GMT 2010
| |
| − | | Mon Nov 15 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Young Adult Cephalic sheath (CEPsh) tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 660
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | Young Adult N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2851
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | Young Adult reference (mockIP) tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 656
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | dauer daf-2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2852
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | dauer entry daf-2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2853
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | dauer exit daf-2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2854
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | early embryo 20dC 0-4hrs post-fertilization N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 476
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | early embryo N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2855
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo A-class motor neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 654
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo AVA neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 459
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo GABA motor neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 468
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo all cells reference tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 456
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo body wall muscle tiling array (v2)
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 470
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo coelomocytes tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 458
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo dopaminergic neurons tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 467
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo germline precursor cells tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 661
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Fri Jun 25 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo him-8 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2856
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo hypodermal cells tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 662
| |
| − | | transcript_identification_design
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Thu Nov 19 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo intestine tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 457
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo panneural tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 455
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo_BAG_neurons_tiling_array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 2499
| |
| − | | transcript_identification_design
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo_PVC_neurons_tiling_array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 2500
| |
| − | | transcript_identification_design
| |
| − | | Tue Feb 16 00:00:00 GMT 2010
| |
| − | | Tue Feb 16 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | embryo_pharyngeal_muscle_tiling_array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 2548
| |
| − | | transcript_identification_design
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Wed Feb 10 00:00:00 GMT 2010
| |
| − | | Mon Nov 08 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | gonad from young adult 20dC 42hrs post-L1 N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 481
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | late embryo 20dC 6-12hrs post-fertilization N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 479
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | late embryo N2 integrated transcripts from RNA-seq, mRNA/EST, RTPCR, mass-spec, polyA sites, and SLs
| |
| − | | Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.
| |
| − | | 2857
| |
| − | | transcript_identification_design
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Thu Feb 18 00:00:00 GMT 2010
| |
| − | | Tue Nov 16 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | male L4 25dC 36hrs post-L1 CB4689 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
| |
| − | | 478
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | mid-L1_miRNA_expression_change
| |
| − | | We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
| |
| − | | 2777
| |
| − | | transcript_identification_design
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Feb 13 00:00:00 GMT 2010
| |
| − | | Sat Nov 13 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | pathogen Efaecalis 25dC 24hr exposure post-adulthood N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls.
| |
| − | | 487
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | pathogen Pluminscens 25dC 24hr exposure post-adulthood N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls.
| |
| − | | 486
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | pathogen Smarcescens 25dC 24hr exposure post-adulthood N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls.
| |
| − | | 489
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | pathogen Smarcescens 25dC 48hr exposure post-adulthood N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls.
| |
| − | | 488
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | pathogen control OP50 25dC 24hr exposure post-adulthood N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls.
| |
| − | | 491
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | pathogen control OP50 25dC 48hr exposure post-adulthood N2 tiling array
| |
| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls.
| |
| − | | 490
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Wed Jun 23 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA 454 sequencing of N2 young adults
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2831
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA 454 sequencing of eri-1(mg366)
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2827
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA 454 sequencing of fer-1 unfertilized oocytes
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2832
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA 454 sequencing of glp-4(bn2)
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2828
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA 454 sequencing of him-8 sperm
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2829
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA Illumina sequencing of N2 embryos
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2826
| |
| − | | transcript_identification_design
| |
| − | | Sun Feb 14 00:00:00 GMT 2010
| |
| − | | Sun Feb 14 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA Illumina sequencing of N2 whole worms
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2825
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA Illumina sequencing of eri-1(mg366)
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2821
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Thu Nov 11 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA Illumina sequencing of fer-1 unfertilized oocytes
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2830
| |
| − | | transcript_identification_design
| |
| − | | Sun Feb 14 00:00:00 GMT 2010
| |
| − | | Sun Feb 14 00:00:00 GMT 2010
| |
| − | | Tue Nov 02 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA Illumina sequencing of glp-4(bn2)
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2822
| |
| − | | transcript_identification_design
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Fri Feb 12 00:00:00 GMT 2010
| |
| − | | Thu Nov 11 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA Illumina sequencing of him-8 sperm
| |
| − | | The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
| |
| − | | 2823
| |
| − | | transcript_identification_design
| |
| − | | Sun Feb 14 00:00:00 GMT 2010
| |
| − | | Sun Feb 14 00:00:00 GMT 2010
| |
| − | | Thu Nov 11 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA N2 embryos RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 634
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA N2 mid-L1 RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 635
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA N2 mid-L2 RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 636
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA N2 mid-L3 RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 637
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA N2 mid-L4 RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 638
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA N2 young adult RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 639
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNA him-8 males RNA-seq
| |
| − | | We aim to examine the changes in expression of non-coding small RNAs, including miRNAs and piRNAs/21U-RNAs, during development and aging, and in the different sexes of C. elegans using high-throughput sequencing technology Solexa. Additionally, we will identify novel small RNAs, especially novel miRNA candidates.
| |
| − | | 640
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Mon Mar 15 00:00:00 GMT 2010
| |
| − | |
| |
| − | |-
| |
| − | | small RNAs from living one cell C. elegans embryos (GSM427345)
| |
| − | | We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.
| |
| − | | 2489
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Tue Aug 17 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | smallRNA from mixed-stage embryos (GSM427346)
| |
| − | | We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.
| |
| − | | 2483
| |
| − | | transcript_identification_design
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Tue Nov 17 00:00:00 GMT 2009
| |
| − | | Mon Aug 16 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | smallRNAs from older, fixed C. elegans embryos (GSM427344)
| |
| − | | We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.
| |
| − | | 2488
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Tue Aug 17 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | smallRNAs from one cell stage C. elegans embryos (GSM427301)
| |
| − | | We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.
| |
| − | | 2486
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Tue Aug 17 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | smallRNAs from post-gastrulation C. elegans embryos (GSM427332)
| |
| − | | We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.
| |
| − | | 2487
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
| |
| − | | Tue Aug 17 01:00:00 BST 2010
| |
| − | |
| |
| − | |-
| |
| − | | smallRNAs from two-to-four cell C. elegans embryos (GSM427297)
| |
| − | | We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.
| |
| − | | 2485
| |
| − | | transcript_identification_design
| |
| − | | Wed Nov 18 00:00:00 GMT 2009
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| − | | Wed Nov 18 00:00:00 GMT 2009
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| − | | Tue Aug 17 01:00:00 BST 2010
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| − | |
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| − | |-
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| − | | soma-only mid-L4 25dC 36hrs post-L1 JK1107 tiling array
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| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
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| − | | 485
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| − | | transcript_identification_design
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| − | | Tue Nov 17 00:00:00 GMT 2009
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| − | | Tue Nov 17 00:00:00 GMT 2009
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| − | | Wed Jun 23 01:00:00 BST 2010
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| − | |
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| − | |-
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| − | | young adult 25dC 42hrs post-L1 N2 tiling array
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| − | | Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
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| − | | 475
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| − | | transcript_identification_design
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| − | | Tue Nov 17 00:00:00 GMT 2009
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| − | | Tue Nov 17 00:00:00 GMT 2009
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| − | | Wed Jun 23 01:00:00 BST 2010
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| | |} | | |} |
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