WormBase Genome Integration Standards
This is a stub so feel free to add content.
At the last ABM we were asked by the board to come up with a list of criteria that a genome assembly/project should attain before WormBase would agree to integrate the Organism into the database.
This topic has re-surfaced as SangerWB have been in contact with the Sanger Helminth group regarding future integration of their data.
They run a pipeline whereby there are 4 phases
1) Production (X months) - No Interest for WB | 2) Finishing (3 Months) - No Interest for WB | 3) Analysis (3 months) - Depending on our criteria we could be interested here | 2) Repeat - Finishing | 3) Repeat - Analysis | x n | 4) Publish when the genome/gene set meets theIR standards
The Helminth Co-ordinator is going to send some information regarding the standards they will be working to but these will be based on the paper "Genome Project Standards in a New Era of Sequencing" so this might be a good starting point for WormBase to base our list of criteria.....(It's not very detailed :( )
expected number of genes
To get an estimated of how many genes could be expected in an assembly, you could take the average gene length and see how many can be at maximum being predicted based on the available contigs (if you can come up with the intergenic percentage you can include that). Take this in correlation to a wild guess on the gene number (like 20k) and you got a % completeness.
average predicted gene length is 2126bp
27210 contigs total 24074 contigs smaller than one gene 1424 contigs with an expected maximum of one gene 333 contigs with an expected maximum of two genes 98 contigs with an expected maximum of three genes 168 contigs with an expected maximum of four genes 195 contigs with an expected maximum of four genes ....
basically assuming no intergenic sequence, you could expect a maximum of 32924 genes in that assembly ... and we predicted 18348.
WormBase Standards Document
Improved High-Quality Draft: