Difference between revisions of "Cosmids/YACs"

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(New page: ==== <span class="mw-headline"</span> ==== <u>'''C. elegans Cosmids and YAC information.'''</u><br> *<span class="mw-headline"></span>There are seven different types of "Clone" objects i...)
 
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==== <u>'''C. elegans Cosmids and YAC information.'''</u><br> ====
  
<u>'''C. elegans Cosmids and YAC information.'''</u><br>
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*<span class="mw-headline"></span>There are seven different types of "Clone" objects in WormBase:<br>
 
*<span class="mw-headline"></span>There are seven different types of "Clone" objects in WormBase:<br>
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<br> Most cosmids, fosmids, YACs can be requested from Sanger, cDNA (yk*) from Dr. Yuji Kohara. The EGAP* plasmids can be obtained from MRC Geneservice. The V* plasmids are no longer available.  
 
<br> Most cosmids, fosmids, YACs can be requested from Sanger, cDNA (yk*) from Dr. Yuji Kohara. The EGAP* plasmids can be obtained from MRC Geneservice. The V* plasmids are no longer available.  
  
 
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*<u>'''Info on clone ends'''</u>
 
*<u>'''Info on clone ends'''</u>
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The caveat with this is that the 'true' end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished).  
 
The caveat with this is that the 'true' end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished).  
  
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*<span style="font-weight: bold;"><u>'''Order Info'''</u></span><span class="mw-headline">  </span>
  
*<span style="font-weight: bold;"><u>'''Order Info'''</u></span><span class="mw-headline">  </span>
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Please send all requests for C.elegans genomic clones to Audrey Fraser (aef@sanger.ac.uk) Please remember to include a FedEx/DHL Account number to which we can charge the shipping cost. Note that the suffixes to YAC names (e.g. Y73B3A, Y73B3B) indicate sequenced segments of the YAC (Y73B3 in this case). They do not exist as subclones. You will be sent the whole YAC.
  
Please send all requests for C.elegans genomic clones to Audrey Fraser (aef@sanger.ac.uk) Please remember to include a FedEx/DHL Account number to which we can charge the shipping cost. Note that the suffixes to YAC names (e.g. Y73B3A, Y73B3B) indicate sequenced segments of the YAC (Y73B3 in this case). They do not exist as subclones. You will be sent the whole YAC.
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Revision as of 16:52, 1 December 2008

C. elegans Cosmids and YAC information.

  • There are seven different types of "Clone" objects in WormBase:
Type Nomenclature
Cosmid A*, B*, C*, D*, F*,J*,K*,M*,R*,T*,W*,Z*
Fosmid H* WRM*
YAC Y*
cDNA yk*, EC*, EB*, OST*, CK*, EF*, CEE*, CEM*, CES*, CB*, CN*, cm*
Plasmid: PCR clones V*,EGAP*
Other telo clones, 1 BAC, plasmid


Most cosmids, fosmids, YACs can be requested from Sanger, cDNA (yk*) from Dr. Yuji Kohara. The EGAP* plasmids can be obtained from MRC Geneservice. The V* plasmids are no longer available.


  • Info on clone ends

The set of clone ends is dumped as part of the gff files:

http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/GFF_files.shtml

This is the source for the extents displayed in WormBase.

The caveat with this is that the 'true' end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished).


  • Order Info

Please send all requests for C.elegans genomic clones to Audrey Fraser (aef@sanger.ac.uk) Please remember to include a FedEx/DHL Account number to which we can charge the shipping cost. Note that the suffixes to YAC names (e.g. Y73B3A, Y73B3B) indicate sequenced segments of the YAC (Y73B3 in this case). They do not exist as subclones. You will be sent the whole YAC.