https://wiki.wormbase.org/index.php?title=BASIC_PROTOCOL_12:_EXAMINING_THE_GENOMIC_CONTENT_OF_A_CLASSICAL_GENETIC_INTERVAL&feed=atom&action=historyBASIC PROTOCOL 12: EXAMINING THE GENOMIC CONTENT OF A CLASSICAL GENETIC INTERVAL - Revision history2024-03-29T01:08:03ZRevision history for this page on the wikiMediaWiki 1.33.0https://wiki.wormbase.org/index.php?title=BASIC_PROTOCOL_12:_EXAMINING_THE_GENOMIC_CONTENT_OF_A_CLASSICAL_GENETIC_INTERVAL&diff=5869&oldid=prevKyook at 19:56, 11 August 20102010-08-11T19:56:31Z<p></p>
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</table>Kyookhttps://wiki.wormbase.org/index.php?title=BASIC_PROTOCOL_12:_EXAMINING_THE_GENOMIC_CONTENT_OF_A_CLASSICAL_GENETIC_INTERVAL&diff=5741&oldid=prevCgrove at 20:40, 10 August 20102010-08-10T20:40:41Z<p></p>
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</table>Cgrovehttps://wiki.wormbase.org/index.php?title=BASIC_PROTOCOL_12:_EXAMINING_THE_GENOMIC_CONTENT_OF_A_CLASSICAL_GENETIC_INTERVAL&diff=41&oldid=prevTharris: New page: Within a defined physical or genetic interval, what gene markers and SNPs are available for mapping a newly identified mutation? WormBase provides a page specifically for this problem. Ne...2007-09-23T20:45:04Z<p>New page: Within a defined physical or genetic interval, what gene markers and SNPs are available for mapping a newly identified mutation? WormBase provides a page specifically for this problem. Ne...</p>
<p><b>New page</b></p><div>Within a defined physical or genetic interval, what gene markers and SNPs are available for mapping a newly identified mutation? WormBase provides a page specifically for this problem.<br />
<br />
Necessary Resources<br />
<br />
Hardware<br />
<br />
A standard computer with a reasonably fast connection to the Internet (cable modem, DSL, or Ethernet recommended)<br />
<br />
Software<br />
<br />
Web browser such as Internet Explorer (http://www.microsoft.com) or Mozilla (http://www.mozilla.org/firefox)<br />
<br />
1. Click the Markers link at the right center top of the WormBase front page (Fig. 1.8.1) to begin a search for genetic markers and their strains (http://wormbase.org/db/searches/strains).<br />
<br />
2. Choose the appropriate markers within an interval of 2 cM or less of a chosen target. Full instructions are given on the page; there are options to exclude subtle, male-sterile, or lethal phenotypes, to select SNPs (Swan et al., 2002) or snip-SNPs (Wicks et al., 2001), or to only show publicly available strains as sources of markers.<br />
<br />
3. Click the Search link and examine the results. In Figure 1.8.19, an example is shown of gene markers in the vicinity of hid-3, an uncloned gene identified by screening for mutants defective in the control of dauer larval development during severe heat (Ailion and Thomas, 2003). A table (Fig. 1.8.20) as well as a graphical map is given.<br />
<br />
Note that instructions for ordering strains are available from the CGC Strains link in the Worm Reagent section of the front page's Links directory (Fig. 1.8.1); presently, this link leads to the e-mail address of Theresa Stiernagle (stier@biosci.cbs.umn.edu) at the University of Minnesota.</div>Tharris