Transgene curation pipeline

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Curating transgenes

Invoke the phenote transgene configuration interface and access postres

go to directory with phenote
 $./phenote -c worm-transgene.cfg

If you want to see all the current 'new' transgenes picked up by Textpresso, go to Tab 3 and press the "Search New Transgene" retrieve button. This action with retrieve all transgene objects that have data in the Summary or Remark fields. Usually there will be paper object info already since it was entered from the Textpresso search.

Curators should look for information of new transgenes in the paper document provided by Textpresso (main paper or supplementary file).

Sometimes papers do not provide any information on the transgenes, only the name is provided. Then "No transgene info in original publication." should be entered into the Remark field so that it will not be identified as a new transgene again.

Here is the controlled vocabulary for the transgene remark field:

• Remark "Conflicting mapping info: ..."
• Remark "Conflicting genotype: ..."
• Remark "No transgene info in original publication."
• Remark "Other integration method: ..."
• Remark "Clone = "
• Remark "Mapping info: "

Phenote transgene.cfg

T= free text; M= multiple values, separate values with a pipe(|); S= selection list

Tab 1

• Pgdbid- postgres database ID, entered automatically when curator enters a new transgene

• Name(T)-approved name following Lab-prefix (or WBPaperID), Is or Ex, number

• Summary(T)- genotype, including co-injection marker and relevant information about making the construct, if papers rport conflicting genotypes use Remark field and controlled vocabulary "Conflicting genotype: ...", if no information enter "No transgene info in original publication." in Remark field.

• Driven by Gene(T,M)- enter WBGeneID used for promoters in every promoter driven construct of the transgene

• Reporter Product(S,M)- list has common reporter genes, GFP, RFP, LacZ, etc.

• Other Reporter(T,M)- enter other products encoded as reporters that do not appear in the drop down list

• Gene(T,M)- enter WBGeneID for protein output of construct, which isn't considered a reporter product

• Integrated by(S)- choose integration method if known, use 'not integrated' for Ex transgenes, if integration method is not listed, use Remark field and controlled vocabulary: "Other integration method: ..."

• Strain(T,M)- not used consistently, enter approved strain names for those strains that contain the transgene

• Map(S,M)- choose LG(s) of integrated array if known, if papers report differing map positions use Remark field and controlled vocabulary "Conflicting mapping info: ..."

Tab 2

• Map Paper(T,M?)- WBPaperID for paper that reports mapping info
• Map Person(T,M)- WBPersonID? or Name, person evidence
• Marker for(T,M)- not used, Wen's expression data
• Marker Paper(T,M)- WBPaperID, not used, Wen's expression data
• Reference(T,M)- WBPaperID, generally autofilled by Textpresso cron job script and bulk upload of Ex search script, add new paper if necessary
• Remark(T,M)- catch all used for clarifying info from other fields, and for entering construct specifics, in some cases use controlled vocabulary
  • "Conflicting mapping info: ..."
  • "Conflicting genotype: ..."
  • "No transgene info in original publication."
  • "Other integration method: ..."
  • "Clone = "
  • "Mapping info: "
• Species(T,M?)- not used?
• Synonym(T,M)- other names for the transgene or construct
• Driven by Construct(T,M)- not sure what this is
• Location(T,M)- Lab designations for people who have the transgene, not sure about this.

Tab 3

• Movie(T)- used?
• Picture(T)- used?
• Search New Transgene(T)- use to retrieve all transgenes that do not have any summary or remark data
• SQL-use?

Transfer from phenote to OA

T= free text; M= multiple values, separate values with a pipe(|); S= selection list

Fields to keep

These are fields that I use, when switching to OA, some can use some modifications.

• Pgdbid->keep - postgres database ID, entered automatically when curator enters a new transgene

• Name(T)->keep -approved name following Lab-prefix (or WBPaperID), Is or Ex, number.

• Summary(T)->keep - genotype, including co-injection marker and relevant information about making the construct, if papers report conflicting genotypes use Remark field and controlled vocabulary "Conflicting genotype: ...", if no information enter "No transgene info in original publication." in Remark field.

• Driven by Gene(T,M)->keep, make selection list, allow multiple entry by public_name convert to WBGeneID based on latest genename server version, make sure all entries are unique - enter WBGeneID used for promoters in every promoter driven construct of the transgene

• Reporter Product(S,M)->keep - list has common reporter genes (heterologous in C. elegans), GFP, RFP, LacZ, etc.

• Other Reporter(T,M)->keep - enter other products encoded as reporters that do not appear in the drop down list

• Gene(T,M)->keep, make selection list, allow multiple entry by public_name convert to WBGeneID based on latest genename server version, make sure all entries are unique - enter WBGeneID for protein output of construct, which isn't considered a reporter product

• Integrated by(S)->keep - choose integration method if known, use 'not integrated' for Ex transgenes, if integration method is not listed, use Remark field and controlled vocabulary: "Other integration method: ..."

• Strain(T,M)->keep - not used consistently, should be strain names for those strains that contain the transgene, but labs have complained about receiving too many requests for the strains(?)

• Map(S,M)->keep, and keep together with other Map fields - choose LG(s) of integrated array if known, if papers report differing map positions use Remark field and controlled vocabulary "Conflicting mapping info: ..."

• Map Paper(T,M?)->keep?, make selection list - WBPaperID for paper that reports mapping info or that performed the mapping?

• Map Person(T,M)->keep- WBPersonID? or Name, person evidence

• Reference(T,M)->keep, move to first tab, make selection field, allow multiples - WBPaperID, generally autofilled by Textpresso cron job script and bulk upload of Ex search script, add new paper if necessary

• Remark(T,M)->keep, move to first tab- catch all used for clarifying info from other fields, and for entering construct specifics, in some cases use controlled vocabulary
  • "Conflicting mapping info: ..."
  • "Conflicting genotype: ..."
  • "No transgene info in original publication."
  • "Other integration method: ..."
  • "Mapping info: "

• Synonym(T,M)->keep - other names for the transgene or construct

• Search New Transgene(T)-> keep- use to retrieve all transgenes that do not have any summary or remark data

Unknown/unused fields

• Marker for(T,M)->not sure if this is still needed, ask Wen" -> Wen's expression data

• Marker Paper(T,M)->same as above- WBPaperID, not used ->Wen's expression data

• Species(T,M?)->used? if this is for species the construct is expressed in, can we make this default C. elegans unless otherwise stated, and can we make this a selection list?

• Driven by Construct(T,M)->ask Wen looks like cell/tissue specific promoter expression information, is this part of Wen's expression curation? Can we make this a cell/tissue ontology based field? is there a need for a life-stage expression driver field as well??

• Location(T,M)->keep?- Lab designations for people who have the transgene--are we still using this? Where is this information extracted from, last author of paper? Can we automatically assign the lab based on the transgene prefix for many of the cases?

• Movie(T)-> used?

• Picture(T)-> used?

• SQL-> used?

Proposed New Fields

• Associated Clone/Plasmid->New field, selection multiple list field, with ability to modify list"

• Co-injection marker-> New field

• Genomic Expression->New field - for developing a standardizing transgene expression nomenclature, eventually we will want to fill this by script composition based on other fields (promoters, reporters, clones)