WS122
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Release Letter
New release of WormBase WS122, Wormpep122 and Wormrna122 March 26th 2004 WS122 was built by Paul ====================================================================== This directory includes: i) database.WS122.*.tar.gz - compressed data for new release ii) models.wrm.WS122 - the latest database schema (also in above database files) iii) CHROMOSOMES/subdir - contains 3 files (DNA, GFF & AGP per chromosome) iv) WS122-WS121.dbcomp - log file reporting difference from last release v) wormpep122.tar.gz - full Wormpep distribution corresponding to WS122 vi) wormrna122.tar.gz - latest WormRNA release containing non-coding RNA's in the genome vii) confirmed_genes.WS122.gz - DNA sequences of all genes confirmed by EST &/or cDNA viii) yk2orf.WS122.gz - Latest set of ORF connections to each Yuji Kohara EST clone ix) gene_interpolated_map_positions.WS122.gz - Interpolated map positions for each coding/RNA gene x) clone_interpolated_map_positions.WS122.gz - Interpolated map positions for each clone xi) best_blastp_hits.WS122.gz - for each C. elegans WormPep protein, lists Best blastp match to human, fly, yeast, C. briggsae, and SwissProt & TrEMBL proteins. xii) best_blastp_hits_brigprot.WS122.gz - for each C. briggsae protein, lists Best blastp match to human, fly, yeast, C. elegans, and SwissProt & TrEMBL proteins. Release notes on the web: ------------------------- http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE Primary databases used in build WS122 ------------------------------------ brigdb : 2004-03-12 - updated camace : 2004-03-15 - updated citace : 2004-03-14 - updated cshace : 2004-03-03 - updated genace : 2004-03-19 - updated stlace : 2004-03-12 - updated Genome sequence composition: ---------------------------- WS122 WS121 change ---------------------------------------------- a 32368547 32368547 +0 c 17781234 17781234 +0 g 17758249 17758249 +0 t 32369937 32369937 +0 n 8 8 +0 - 0 0 +0 Total 100277975 100277975 +0 Wormpep data set: ---------------------------- There are 19895 CDS in autoace, 22264 when counting 2369 alternate splice forms. The 22264 sequences contain 9,767,313 base pairs in total. Modified entries 45 Deleted entries 9 New entries 12 Reappeared entries 7 Net change +10 Status of entries: Confidence level of prediction (based on the amount of transcript evidence) ------------------------------------------------- Confirmed 4870 (21.9%) Every base of every exon has transcription evidence (mRNA, EST etc.) Partially_confirmed 12049 (54.1%) Some, but not all exon bases are covered by transcript evidence Predicted 5345 (24.0%) No transcriptional evidence at all Status of entries: Protein Accessions ------------------------------------- Swissprot accessions 2425 (10.9%) TrEMBL accessions 18443 (82.8%) TrEMBLnew accessions 1236 ( 5.6%) Status of entries: Protein_ID's in EMBL --------------------------------------- Protein_id 22212 (99.8%) Locus <-> Sequence connections (cgc-approved) --------------------------------------------- Entries with locus connection 4973 GeneModel correction progress WS121 -> WS122 ----------------------------------------- Confirmed introns not in a CDS gene model; +---------+--------+ | Introns | Change | +---------+--------+ Cambridge | 419 | -10 | St Louis | 278 | 19 | +---------+--------+ Members of known repeat families that overlap predicted exons; +---------+--------+ | Repeats | Change | +---------+--------+ Cambridge | 662 | 662 | St Louis | 1028 | 1028 | +---------+--------+ Synchronisation with GenBank / EMBL: ------------------------------------ CHROMOSOME_II sequence Z46676 There are no gaps remaining in the genome sequence For more info mail help@wormbase.org -===================================================================================- New Data: --------- The SAGE analysis from Jones et al has been incorporated into this release. There are approximately 35,000 SAGE tags in the dataset. Marker loci on the genetic map have been partioned into those which have been experimentally defined (displayed in yellow) and those which are computationally inferred (displayed in orange). New Fixes: ---------- Known Problems: -------------- Other Changes: -------------- The script that check overlaps between coding exons and repeat sequences now uses the RepeatMasker data. Hence, the numbers have increased significantly. There are two caveats to these numbers; Firstly, at the moment these include the low-complexity mark up of RepeatMasker which we will in future ignore, this will reduce the numbers by approximately a third. Secondly, the latest RepeatMasker library still contains some motifs from multiple-gene families and hence overestimates the number of genes which need attention. The curators will work through this list to remove these motifs from the repeat library. Proposed Changes / Forthcoming Data: ------------------------------------ -===================================================================================- Quick installation guide for UNIX/Linux systems ----------------------------------------------- 1. Create a new directory to contain your copy of WormBase, e.g. /users/yourname/wormbase 2. Unpack and untar all of the database.*.tar.gz files into this directory. You will need approximately 2-3 Gb of disk space. 3. Obtain and install a suitable acedb binary for your system (available from www.acedb.org). 4. Use the acedb 'xace' program to open your database, e.g. type 'xace /users/yourname/wormbase' at the command prompt. 5. See the acedb website for more information about acedb and using xace. ____________ END _____________